O51349 (RPOC_BORBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta'
Short name=RNAP subunit beta'
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta'
Transcriptase subunit beta'

Gene names

Name:	rpoC
Ordered Locus Names:	BB_0388

Organism

Borrelia burgdorferi (Lyme disease spirochete)

Taxonomic identifier

139 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	1377 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01322
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01322
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta' chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1377	1377	DNA-directed RNA polymerase subunit beta' HAMAP MF_01322		PRO_0000067711
Experimental info
	Sequence conflict	17	1	P → S Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O51349 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 160DD492579ED4D0	FASTA	1,377	154,658
	10 20 30 40 50 60 MKEIKDFERI KIKIASPDQI RNWSYGEVKK SETINYRTLR PEKDGLFCER IFGTTKEWEC 70 80 90 100 110 120 YCGKFKSVRY KGIICDRCNV EVTHFKVRRE RMGHIELAAP VAHIWYYKYI PSRIGLLLDI 130 140 150 160 170 180 TASSLNSILY YEKYVVIEPG DTDLKKMQLL NEDEYIEARE RYGMSFNASM GAEAIKTLLE 190 200 210 220 230 240 NLDLDELSSK LRIQMIDKDD KTDKKLLRRL EIIENFKISG NKPEWMIMEV LPVIPPEIRP 250 260 270 280 290 300 MVQLDGGRFA TSDLNDLYRR VINRNNRLRK LLLLNAPEII VRNEKRMLQE SVDSLFDNSH 310 320 330 340 350 360 KRKVVKGSSS RPLKSLSDAL KGKQGRFRQN LLGKRVDYSG RSVIVVGPEL KLHQCGLPAK 370 380 390 400 410 420 MALELFKPFV IRRLIESEAV FNIKRAKNLI EQEVDEVWQI LDLVIKEHPI LLNRAPTLHR 430 440 450 460 470 480 LGIQAFEPVL VEGKAIKLHP LVCHAYNADF DGDQMAVHVP LTPAAQAESW ALMLSTNNLL 490 500 510 520 530 540 NPANGHPIVF PSQDIVLGLY YLTMEKKNVV GEGKKFLNFN NVILAINNRS LDYNASIYVK 550 560 570 580 590 600 IHGEYKKTTA GRVIFNEALP KGIEFVNKTL SDLELQILIS KVYVVHGSSI VIEMLDIIKE 610 620 630 640 650 660 LGFRYATKFG CTISMSDIIV PDEKRTYVER ANKEIAKIQN DYAKGVITGE ERYNNVVSVW 670 680 690 700 710 720 LKTNEELTNK MMEILKKDRD GFNVIYMMAD SGARGSRNQI RQLAGMRGLM AKTSGDIIEL 730 740 750 760 770 780 PIISNFKEGL SVIEFFISTN GARKGLADTA LKTADAGYLT RRLVDIAQDV VVRIEDCGTI 790 800 810 820 830 840 NGIKVETVKN GEEILESLKE KAVGSYSIER IKNPITGEIV LDANEEISEA KIELLEKIGI 850 860 870 880 890 900 EKLVIRSVLT CEAEHGVCQK CYGRDFSKNK PVNIGEAVGI IAAQSIGQPG TQLTMRTFHI 910 920 930 940 950 960 GGVAQAGSED DKISLKNAFI LNGIEGFNVR VDNGILFTRK GTLKIINVFY EEKIKNIKEI 970 980 990 1000 1010 1020 KVLDSQRVIK GIPLFIDKKG SEILSSYIGY VKLRDDNFFI VSEEQEVSLK AGTKLEIEVG 1030 1040 1050 1060 1070 1080 DYVESGKVIG TFDPFAEPII AEVKGKIKFK DIILGTTLKE EINTETGNVE KRITDNVFES 1090 1100 1110 1120 1130 1140 LDPRIFIIDS SGMEVASYVL PGDAYLQVED GQSINIGDII AKLSKGSEKT QDITGGLPRV 1150 1160 1170 1180 1190 1200 NDLFETRIPK NLTEMAKVSG IVQFKSIQKG KRLINILDEY GVEHKHYIPA GKHLLVRDGD 1210 1220 1230 1240 1250 1260 VVKAGDMLCD GRINPHDVLE ILGGISLQEF LLAEIQDVYR KQGVSINDKH IGVIIKQMMK 1270 1280 1290 1300 1310 1320 KVKIVAVGDT NFVYGQKVDK HTFYEQNRKV IEQGGEPAIA SPILIGVTKT SLNIDSFISA 1330 1340 1350 1360 1370 ASFQETTKVL TDASIAGKID DLRGLKENVV IGHLIPTGTG MGLYKKIKVS ENIDSEV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi." Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L., Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B., Smith H.O., Venter J.C. Nature 390:580-586(1997) [PubMed: 9403685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.
[2]	"Molecular cloning and characterization of Borrelia burgdorferi rpoB." Alekshun M., Kashlev M., Schwartz I. Gene 186:227-235(1997) [PubMed: 9074501] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-19. Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000783 Genomic DNA. Translation: AAB91502.1. L48488 Genomic DNA. Translation: AAC37119.1.
PIR	C70148.
RefSeq	NP_212522.1. NC_001318.1.
3D structure databases
ProteinModelPortal	O51349.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBORT00000008887; EBBORP00000008217; EBBORG00000008886.
GeneID	1195225.
GenomeReviews	Gene locus BB_0388 in contig AE000783_GR.
KEGG	bbu:BB0388.
NMPDR	fig|224326.1.peg.772.
PATRIC	20557386. VBIBorBur75917_0783.
TIGR	BB_0388.
Phylogenomic databases
GeneTree	EBGT00050000007232.
HOGENOM	HBG621785.
OMA	DINDKHI.
PhylomeDB	O51349.
ProtClustDB	PRK00566.
Enzyme and pathway databases
BioCyc	BBUR224326:BB_0388-MONOMER.
Family and domain databases
HAMAP	MF_01322. RNApol_bact_RpoC. [Tree]
InterPro	IPR012754. DNA-dir_RpoC_beta_prime. IPR000722. RNA_pol_asu. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. [Graphical view]
KO	K03046.
Pfam	PF04997. RNA_pol_Rpb1_1. 1 hit. PF00623. RNA_pol_Rpb1_2. 1 hit. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR02386. RpoC_TIGR. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RPOC_BORBU

Accession

Primary (citable) accession number: O51349
Secondary accession number(s): Q45035

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	January 25, 2012
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program