Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O51345

- SYE_BORBU

UniProt

O51345 - SYE_BORBU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei254 – 2541ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBBUR224326:G9SO-369-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:BB_0372
OrganismiBorrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Taxonomic identifieri224326 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorreliaBorrelia burgdorferi group
ProteomesiUP000001807: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 490490Glutamate--tRNA ligasePRO_0000119517Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi224326.BB0372.

Structurei

Secondary structure

1
490
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 74
Beta strandi11 – 133
Helixi17 – 3216
Beta strandi36 – 394
Helixi51 – 6414
Beta strandi69 – 713
Turni72 – 743
Helixi83 – 853
Helixi87 – 9913
Beta strandi102 – 1065
Helixi110 – 12213
Turni132 – 1354
Helixi138 – 1469
Beta strandi152 – 1554
Beta strandi162 – 1676
Turni168 – 1703
Beta strandi171 – 1766
Helixi177 – 1793
Beta strandi185 – 1873
Helixi195 – 20511
Beta strandi209 – 2146
Helixi215 – 2206
Helixi221 – 23111
Beta strandi237 – 2415
Beta strandi249 – 2513
Turni254 – 2563
Helixi261 – 2677
Helixi271 – 28010
Beta strandi281 – 2833
Beta strandi285 – 2884
Helixi294 – 3007
Helixi303 – 3053
Helixi315 – 32814
Helixi331 – 34414
Helixi354 – 36815
Helixi376 – 3805
Helixi382 – 3854
Helixi393 – 3953
Helixi405 – 41713
Helixi420 – 4223
Helixi425 – 43814
Helixi444 – 45411
Beta strandi455 – 4595
Helixi463 – 4708
Helixi472 – 48615

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GRIX-ray2.60A/B2-490[»]
ProteinModelPortaliO51345.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi9 – 1911"HIGH" regionAdd
BLAST
Motifi251 – 2555"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
KOiK01885.
OMAiVTGQTHG.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

O51345-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTRVRYAPS PTGLQHIGGI RTALFNYFFA KSCGGKFLLR IEDTDQSRYS
60 70 80 90 100
PEAENDLYSS LKWLGISFDE GPVVGGDYAP YVQSQRSAIY KQYAKYLIES
110 120 130 140 150
GHAYYCYCSP ERLERIKKIQ NINKMPPGYD RHCRNLSNEE VENALIKKIK
160 170 180 190 200
PVVRFKIPLE GDTSFDDILL GRITWANKDI SPDPVILKSD GLPTYHLANV
210 220 230 240 250
VDDYLMKITH VLRAQEWVSS GPLHVLLYKA FKWKPPIYCH LPMVMGNDGQ
260 270 280 290 300
KLSKRHGSTA LRQFIEDGYL PEAIINYVTL LGWSYDDKRE FFSKNDLEQF
310 320 330 340 350
FSIEKINKSP AIFDYHKLDF FNSYYIREKK DEDLFNLLLP FFQKKGYVSK
360 370 380 390 400
PSTLEENQKL KLLIPLIKSR IKKLSDALNM TKFFYEDIKS WNLDEFLSRK
410 420 430 440 450
KTAKEVCSIL ELIKPILEGF EKRSSEENDK IFYDFAESNG FKLGEILLPI
460 470 480 490
RIAALGSKVS PPLFDSLKLI GKSKVFERIK LAQEFLRINE
Length:490
Mass (Da):56,752
Last modified:June 1, 1998 - v1
Checksum:iE7ED6AD2474934F4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000783 Genomic DNA. Translation: AAC66742.1.
PIRiC70146.
RefSeqiNP_212506.1. NC_001318.1.

Genome annotation databases

EnsemblBacteriaiAAC66742; AAC66742; BB_0372.
GeneIDi1195209.
KEGGibbu:BB_0372.
PATRICi20557354. VBIBorBur75917_0767.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000783 Genomic DNA. Translation: AAC66742.1 .
PIRi C70146.
RefSeqi NP_212506.1. NC_001318.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4GRI X-ray 2.60 A/B 2-490 [» ]
ProteinModelPortali O51345.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224326.BB0372.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC66742 ; AAC66742 ; BB_0372 .
GeneIDi 1195209.
KEGGi bbu:BB_0372.
PATRICi 20557354. VBIBorBur75917_0767.

Phylogenomic databases

eggNOGi COG0008.
KOi K01885.
OMAi VTGQTHG.
OrthoDBi EOG6DRPF7.

Enzyme and pathway databases

BioCyci BBUR224326:G9SO-369-MONOMER.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.

Entry informationi

Entry nameiSYE_BORBU
AccessioniPrimary (citable) accession number: O51345
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: October 29, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3