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O51345

- SYE_BORBU

UniProt

O51345 - SYE_BORBU

Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei254 – 2541ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBBUR224326:G9SO-369-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetaseUniRule annotation
    Short name:
    GluRSUniRule annotation
    Gene namesi
    Name:gltXUniRule annotation
    Ordered Locus Names:BB_0372
    OrganismiBorrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
    Taxonomic identifieri224326 [NCBI]
    Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorreliaBorrelia burgdorferi group
    ProteomesiUP000001807: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 490490Glutamate--tRNA ligasePRO_0000119517Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi224326.BB0372.

    Structurei

    Secondary structure

    1
    490
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 74
    Beta strandi11 – 133
    Helixi17 – 3216
    Beta strandi36 – 394
    Helixi51 – 6414
    Beta strandi69 – 713
    Turni72 – 743
    Helixi83 – 853
    Helixi87 – 9913
    Beta strandi102 – 1065
    Helixi110 – 12213
    Turni132 – 1354
    Helixi138 – 1469
    Beta strandi152 – 1554
    Beta strandi162 – 1676
    Turni168 – 1703
    Beta strandi171 – 1766
    Helixi177 – 1793
    Beta strandi185 – 1873
    Helixi195 – 20511
    Beta strandi209 – 2146
    Helixi215 – 2206
    Helixi221 – 23111
    Beta strandi237 – 2415
    Beta strandi249 – 2513
    Turni254 – 2563
    Helixi261 – 2677
    Helixi271 – 28010
    Beta strandi281 – 2833
    Beta strandi285 – 2884
    Helixi294 – 3007
    Helixi303 – 3053
    Helixi315 – 32814
    Helixi331 – 34414
    Helixi354 – 36815
    Helixi376 – 3805
    Helixi382 – 3854
    Helixi393 – 3953
    Helixi405 – 41713
    Helixi420 – 4223
    Helixi425 – 43814
    Helixi444 – 45411
    Beta strandi455 – 4595
    Helixi463 – 4708
    Helixi472 – 48615

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4GRIX-ray2.60A/B2-490[»]
    ProteinModelPortaliO51345.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi9 – 1911"HIGH" regionAdd
    BLAST
    Motifi251 – 2555"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0008.
    KOiK01885.
    OMAiVTGQTHG.
    OrthoDBiEOG6DRPF7.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O51345-1 [UniParc]FASTAAdd to Basket

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    MSTRVRYAPS PTGLQHIGGI RTALFNYFFA KSCGGKFLLR IEDTDQSRYS    50
    PEAENDLYSS LKWLGISFDE GPVVGGDYAP YVQSQRSAIY KQYAKYLIES 100
    GHAYYCYCSP ERLERIKKIQ NINKMPPGYD RHCRNLSNEE VENALIKKIK 150
    PVVRFKIPLE GDTSFDDILL GRITWANKDI SPDPVILKSD GLPTYHLANV 200
    VDDYLMKITH VLRAQEWVSS GPLHVLLYKA FKWKPPIYCH LPMVMGNDGQ 250
    KLSKRHGSTA LRQFIEDGYL PEAIINYVTL LGWSYDDKRE FFSKNDLEQF 300
    FSIEKINKSP AIFDYHKLDF FNSYYIREKK DEDLFNLLLP FFQKKGYVSK 350
    PSTLEENQKL KLLIPLIKSR IKKLSDALNM TKFFYEDIKS WNLDEFLSRK 400
    KTAKEVCSIL ELIKPILEGF EKRSSEENDK IFYDFAESNG FKLGEILLPI 450
    RIAALGSKVS PPLFDSLKLI GKSKVFERIK LAQEFLRINE 490
    Length:490
    Mass (Da):56,752
    Last modified:June 1, 1998 - v1
    Checksum:iE7ED6AD2474934F4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000783 Genomic DNA. Translation: AAC66742.1.
    PIRiC70146.
    RefSeqiNP_212506.1. NC_001318.1.

    Genome annotation databases

    EnsemblBacteriaiAAC66742; AAC66742; BB_0372.
    GeneIDi1195209.
    KEGGibbu:BB_0372.
    PATRICi20557354. VBIBorBur75917_0767.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000783 Genomic DNA. Translation: AAC66742.1 .
    PIRi C70146.
    RefSeqi NP_212506.1. NC_001318.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4GRI X-ray 2.60 A/B 2-490 [» ]
    ProteinModelPortali O51345.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224326.BB0372.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC66742 ; AAC66742 ; BB_0372 .
    GeneIDi 1195209.
    KEGGi bbu:BB_0372.
    PATRICi 20557354. VBIBorBur75917_0767.

    Phylogenomic databases

    eggNOGi COG0008.
    KOi K01885.
    OMAi VTGQTHG.
    OrthoDBi EOG6DRPF7.

    Enzyme and pathway databases

    BioCyci BBUR224326:G9SO-369-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.

    Entry informationi

    Entry nameiSYE_BORBU
    AccessioniPrimary (citable) accession number: O51345
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3