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Protein

Tyrosine--tRNA ligase

Gene

tyrS

Organism
Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).UniRule annotation

Catalytic activityi

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 351TyrosineUniRule annotation
Binding sitei166 – 1661TyrosineUniRule annotation
Binding sitei170 – 1701TyrosineUniRule annotation
Binding sitei229 – 2291ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciBBUR224326:G9SO-367-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine--tRNA ligaseUniRule annotation (EC:6.1.1.1UniRule annotation)
Alternative name(s):
Tyrosyl-tRNA synthetaseUniRule annotation
Short name:
TyrRSUniRule annotation
Gene namesi
Name:tyrSUniRule annotation
Ordered Locus Names:BB_0370
OrganismiBorrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Taxonomic identifieri224326 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesBorreliaceaeBorreliella
Proteomesi
  • UP000001807 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 405405Tyrosine--tRNA ligasePRO_0000055645Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi224326.BB_0370.

Structurei

3D structure databases

ProteinModelPortaliO51343.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini340 – 40566S4 RNA-bindingUniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 4910"HIGH" region
Motifi226 – 2305"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily.UniRule annotation
Contains 1 S4 RNA-binding domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DA0. Bacteria.
COG0162. LUCA.
KOiK01866.
OMAiGKHFPVN.

Family and domain databases

Gene3Di3.10.290.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_02006. Tyr_tRNA_synth_type1. 1 hit.
InterProiIPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002942. S4_RNA-bd.
IPR002307. Tyr-tRNA-ligase.
IPR024088. Tyr-tRNA-ligase_bac-type.
IPR024107. Tyr-tRNA-ligase_bac_1.
[Graphical view]
PANTHERiPTHR11766. PTHR11766. 1 hit.
PfamiPF01479. S4. 1 hit.
PF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01040. TRNASYNTHTYR.
SMARTiSM00363. S4. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00234. tyrS. 1 hit.
PROSITEiPS50889. S4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O51343-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLALSLLHK RGFLKQCTSL KVLSDLMDRE KIVFYAGVDA TSSSLHIGHL
60 70 80 90 100
IPFLAMMHLR QHGHMPIVLI GDSTAKIGDP SGKSEMRKIL SSEEIGNNAL
110 120 130 140 150
LIKNQLQRIT KFTSECFIHN SNWLDNLNYI EFLRDVGMHF SVNRMLSFET
160 170 180 190 200
YKRRMDFGLS FIEFNYQLLQ SYDYYMLNKI KNCRLQIGGD DQWGNIISGV
210 220 230 240 250
DLIRKKNGSE TFGLTFPLIT RSDGKKMGKS EKGAVYLDSN LFSIYDFYQY
260 270 280 290 300
FRNTSDSDVK TFLYLFTFLE EDEIELISNF KGNSLNKAKE ILAFEITKIV
310 320 330 340 350
HGEAEALKVQ EASFAAFRGS GDRSNIPFFK FSFSSLKEEI LLVDLMLDSK
360 370 380 390 400
IVPSKSEGRR LIDSGGVYIN GKRVESQSHL LTKKDFNNNE VELRVGKKKF

LRIVI
Length:405
Mass (Da):46,363
Last modified:June 1, 1998 - v1
Checksum:iF6DED7B6F1F2EBBF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000783 Genomic DNA. Translation: AAC66744.1.
PIRiA70146.
RefSeqiNP_212504.1. NC_001318.1.
WP_002656396.1. NC_001318.1.

Genome annotation databases

EnsemblBacteriaiAAC66744; AAC66744; BB_0370.
GeneIDi1195207.
KEGGibbu:BB_0370.
PATRICi20557350. VBIBorBur75917_0765.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000783 Genomic DNA. Translation: AAC66744.1.
PIRiA70146.
RefSeqiNP_212504.1. NC_001318.1.
WP_002656396.1. NC_001318.1.

3D structure databases

ProteinModelPortaliO51343.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224326.BB_0370.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC66744; AAC66744; BB_0370.
GeneIDi1195207.
KEGGibbu:BB_0370.
PATRICi20557350. VBIBorBur75917_0765.

Phylogenomic databases

eggNOGiENOG4105DA0. Bacteria.
COG0162. LUCA.
KOiK01866.
OMAiGKHFPVN.

Enzyme and pathway databases

BioCyciBBUR224326:G9SO-367-MONOMER.

Family and domain databases

Gene3Di3.10.290.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_02006. Tyr_tRNA_synth_type1. 1 hit.
InterProiIPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002942. S4_RNA-bd.
IPR002307. Tyr-tRNA-ligase.
IPR024088. Tyr-tRNA-ligase_bac-type.
IPR024107. Tyr-tRNA-ligase_bac_1.
[Graphical view]
PANTHERiPTHR11766. PTHR11766. 1 hit.
PfamiPF01479. S4. 1 hit.
PF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01040. TRNASYNTHTYR.
SMARTiSM00363. S4. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00234. tyrS. 1 hit.
PROSITEiPS50889. S4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYY_BORBU
AccessioniPrimary (citable) accession number: O51343
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: September 7, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.