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Protein

Pyruvate kinase

Gene

pyk

Organism
Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA)
  4. Enolase (eno)
  5. Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34SubstrateBy similarity1
Metal bindingi36PotassiumBy similarity1
Metal bindingi64PotassiumBy similarity1
Metal bindingi65Potassium; via carbonyl oxygenBy similarity1
Sitei214Transition state stabilizerBy similarity1
Metal bindingi216MagnesiumBy similarity1
Binding sitei239Substrate; via amide nitrogenBy similarity1
Metal bindingi240MagnesiumBy similarity1
Binding sitei240Substrate; via amide nitrogenBy similarity1
Binding sitei272SubstrateBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pyk
Ordered Locus Names:BB_0348
OrganismiBorrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Taxonomic identifieri224326 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesBorreliaceaeBorreliella
Proteomesi
  • UP000001807 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001120571 – 477Pyruvate kinaseAdd BLAST477

Proteomic databases

PRIDEiO51323.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi224326.BB_0348.

Structurei

3D structure databases

ProteinModelPortaliO51323.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiENOG4105CA9. Bacteria.
COG0469. LUCA.
KOiK00873.
OMAiVLCGMMT.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O51323-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISKLTKIVA TISDLRCEPE HIKDLHDAGV NVIRLNTAHQ SHEDTIKVID
60 70 80 90 100
NVRKISNKIA LMIDTKGPEV RTANIENPII VKTGDKVIIS TSPINEPNNF
110 120 130 140 150
QTNYDGFVKE VPQGSKVLID DGELEMTVVA KLPDRLICEI KNDGQIKNKK
160 170 180 190 200
SINTPGISLK LQSVTEKDKG FIELAAKYNV DFIAHSFVRH SKDVQDVQEI
210 220 230 240 250
LTASGNPDVK IISKIENQEG IDNIEEIAKA SYGIMVARGD MGVEIPAEDV
260 270 280 290 300
PIAQLKITQT CIKYGIPVIT ATQMLHTMIE NPRPTRAEVS DIANAILNGT
310 320 330 340 350
DAIMLSGETA YGKYPIEAVK MMTSIAKKVE KHRKMTLYKD ELFYDKSITR
360 370 380 390 400
NYIIKCAIDA TKLMDIKAII VDSLKGKTAR IMATYRASVP LFITTNSERL
410 420 430 440 450
ARELALSYGV YSNLVDNNFK RTTEFVVTSL KMLKEQGVVN DKDTVIIISG
460 470
NPNRNIEKGT EFMEINTVED AIKGRNI
Length:477
Mass (Da):53,032
Last modified:June 1, 1998 - v1
Checksum:i40D3CCAC9ACB7D98
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000783 Genomic DNA. Translation: AAC66733.1.
PIRiC70143.
RefSeqiNP_212482.1. NC_001318.1.
WP_002661716.1. NC_001318.1.

Genome annotation databases

EnsemblBacteriaiAAC66733; AAC66733; BB_0348.
GeneIDi1195185.
KEGGibbu:BB_0348.
PATRICi20557308. VBIBorBur75917_0744.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000783 Genomic DNA. Translation: AAC66733.1.
PIRiC70143.
RefSeqiNP_212482.1. NC_001318.1.
WP_002661716.1. NC_001318.1.

3D structure databases

ProteinModelPortaliO51323.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224326.BB_0348.

Proteomic databases

PRIDEiO51323.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC66733; AAC66733; BB_0348.
GeneIDi1195185.
KEGGibbu:BB_0348.
PATRICi20557308. VBIBorBur75917_0744.

Phylogenomic databases

eggNOGiENOG4105CA9. Bacteria.
COG0469. LUCA.
KOiK00873.
OMAiVLCGMMT.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKPYK_BORBU
AccessioniPrimary (citable) accession number: O51323
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: November 2, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.