O51317 (GATA_BORBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit A
Short name=Glu-ADT subunit A
EC=6.3.5.-

Gene names

Name:	gatA
Ordered Locus Names:	BB_0342

Organism

Borrelia burgdorferi (Lyme disease spirochete)

Taxonomic identifier

139 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) By similarity. HAMAP MF_00120
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP MF_00120
Subunit structure	Heterotrimer of A, B and C subunits By similarity. HAMAP MF_00120
Sequence similarities	Belongs to the amidase family. GatA subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbon-nitrogen ligase activity, with glutamine as amido-N-donor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 496	496	Glutamyl-tRNA(Gln) amidotransferase subunit A HAMAP MF_00120		PRO_0000105141
Sites
	Active site	93	1	Charge relay system By similarity
	Active site	168	1	Charge relay system By similarity
	Active site	192	1	Acyl-ester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O51317 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: D375DEE20DD42710	FASTA	496	55,545
	10 20 30 40 50 60 MDIFHLLKYW SKDKVLDLSN LTLTKIQELV LTRKCKIYDI LLAYKNNYEL NKDINGYIEF 70 80 90 100 110 120 FDDSLEIAKR YDDCLKNCEL EDLPLIGMLI AVKDNISIQD KSLTCASEIL KGYISPYDAT 130 140 150 160 170 180 VIKRLKNKGA ILIGRTNMDE FAMGSTCEFS YYGATLNPLN REYVIGGSSG GSAAVVAAFQ 190 200 210 220 230 240 APFSLGSDTG GSVRLPASFS GILGFKPSYG GLSRYGLASY ASSFDQIGFF SHSIEDIALI 250 260 270 280 290 300 LKHTCGSDKM DSTSVDIFDD FYPLKIESLQ GKNLAVIKEL SEDLMDKNVA NSFAKFKLDL 310 320 330 340 350 360 LSKGINIKEV SIEEINFILS IYYIISPVEA SSNLARYTGL CYGKRISEGL SLNDFYFKHR 370 380 390 400 410 420 SNFLSEEVKR RIVLGNYLLS ERYDSKYYAK ACEILQNLII PKFNKLFESC DFIITPTSFV 430 440 450 460 470 480 KPFRLGLDFD DPVKMYYSDI CTVIANLIGA PAISLPYSKD EEGLSIGMQI IGRSKKDFEL 490 LSFSKNVIRE LGLNGI

References

[1]

"Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi." Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L., Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B., Smith H.O., Venter J.C. Nature 390:580-586(1997) [PubMed: 9403685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000783 Genomic DNA. Translation: AAC66715.1.
PIR	E70142.
RefSeq	NP_212476.1. NC_001318.1.
3D structure databases
ProteinModelPortal	O51317.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBORT00000009009; EBBORP00000008339; EBBORG00000009008.
GeneID	1195179.
GenomeReviews	Gene locus BB_0342 in contig AE000783_GR.
KEGG	bbu:BB0342.
NMPDR	fig|224326.1.peg.726.
PATRIC	20557296. VBIBorBur75917_0738.
TIGR	BB_0342.
Phylogenomic databases
GeneTree	EBGT00050000007061.
HOGENOM	HBG481888.
OMA	ICTVIAN.
PhylomeDB	O51317.
ProtClustDB	PRK00012.
Enzyme and pathway databases
BioCyc	BBUR224326:BB_0342-MONOMER.
Family and domain databases
HAMAP	MF_00120. GatA. [Tree]
InterPro	IPR000120. Amidase. IPR020556. Amidase_CS. IPR023631. Amidase_dom. IPR004412. GatA. [Graphical view]
Gene3D	G3DSA:3.90.1300.10. Amidase_sig_enz. 1 hit.
KO	K02433.
PANTHER	PTHR11895. Amidase. 1 hit.
Pfam	PF01425. Amidase. 1 hit. [Graphical view]
SUPFAM	SSF75304. Amidase_sig_enz. 1 hit.
TIGRFAMs	TIGR00132. GatA. 1 hit.
PROSITE	PS00571. AMIDASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATA_BORBU

Accession

Primary (citable) accession number: O51317

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	June 1, 1998
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program