O51293 (RLME_BORBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase E
EC=2.1.1.166

Alternative name(s):
23S rRNA Um2552 methyltransferase
rRNA (uridine-2'-O-)-methyltransferase

Gene names

Name:	rlmE
Synonyms:	ftsJ, rrmJ
Ordered Locus Names:	BB_0313

Organism

Borrelia burgdorferi (Lyme disease spirochete)

Taxonomic identifier

139 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	192 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit By similarity. HAMAP MF_01547
Catalytic activity	S-adenosyl-L-methionine + uridine(2552) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methyluridine(2552) in 23S rRNA. HAMAP MF_01547
Subcellular location	Cytoplasm By similarity HAMAP MF_01547.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmE family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	RNA methylation Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 192	192	Ribosomal RNA large subunit methyltransferase E HAMAP MF_01547		PRO_0000155475
Sites
	Active site	147	1	Proton acceptor By similarity
	Binding site	48	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	50	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	67	1	S-adenosyl-L-methionine By similarity
	Binding site	85	1	S-adenosyl-L-methionine By similarity
	Binding site	107	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O51293 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 8CAE7FF26CAA8F57	FASTA	192	22,167
	10 20 30 40 50 60 MYRLDDEYSK KAKREGYLAR SVYKLIEINE KFSLFSSGNV LDIGASPGSF SQYAYKKLKR 70 80 90 100 110 120 GILVSVDIND IGLRYDDNFY FIKGDIFLDD TVFKINTFKP YSLVISDVAP KTTGNRLVDT 130 140 150 160 170 180 SNSFNLSMRI IDLSLEVLLK KGNLLVKVFQ GGDEMQIFKK FEKYFKFVKK IRPKAVRKNS 190 FEIYFLGKSF GK

References

[1]

"Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi." Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L., Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B., Smith H.O., Venter J.C. Nature 390:580-586(1997) [PubMed: 9403685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000783 Genomic DNA. Translation: AAC66697.1.
PIR	H70138.
RefSeq	NP_212447.1. NC_001318.1.
3D structure databases
ProteinModelPortal	O51293.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBORT00000009008; EBBORP00000008338; EBBORG00000009007.
GeneID	1195150.
GenomeReviews	Gene locus BB_0313 in contig AE000783_GR.
KEGG	bbu:BB0313.
NMPDR	fig|224326.1.peg.697.
PATRIC	20557242. VBIBorBur75917_0711.
TIGR	BB_0313.
Phylogenomic databases
GeneTree	EBGT00050000007024.
HOGENOM	HBG398270.
OMA	REIYLIC.
ProtClustDB	CLSK507509.
Enzyme and pathway databases
BioCyc	BBUR224326:BB_0313-MONOMER.
Family and domain databases
HAMAP	MF_01547. 23SrRNA_methyltr_E. [Tree]
InterPro	IPR015507. rRNA-MeTfrase_E. IPR002877. rRNA_MeTrfase_RrmJ/FtsJ. [Graphical view]
KO	K02427.
PANTHER	PTHR10920. rRNA-MeTfrase_J. 1 hit.
Pfam	PF01728. FtsJ. 1 hit. [Graphical view]
PIRSF	PIRSF005461. 23S_rRNA_mtase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLME_BORBU

Accession

Primary (citable) accession number: O51293

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	June 1, 1998
Last modified:	January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program