O51263 (NTPA_BORBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

BB_0247

Organism

Borrelia burgdorferi (Lyme disease spirochete)

Taxonomic identifier

139 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 201	201	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_0000178136
Regions
	Region	8 – 13	6	Substrate binding By similarity
	Region	68 – 69	2	Substrate binding By similarity
Sites
	Metal binding	68	1	Magnesium or manganese By similarity
	Binding site	158	1	Substrate By similarity
	Binding site	177	1	Substrate By similarity
	Binding site	183	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O51263 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 199E43E0704B4575	FASTA	201	23,138
	10 20 30 40 50 60 MKTLFFATTN ENKINEVKNI LDIPNLNLIV PQNFNIKETG KTFKENSLLK AKALFEILNN 70 80 90 100 110 120 NQNVFGEDSG LCIEALNLEP GIYSKRYDTY KLCKKLSTNE KNQLILDLMK NEKNRKAYFI 130 140 150 160 170 180 CNISYISKNG QILNFEGIIK GKIALSLNDK KNYGFGYDSI FLTKNNKKLS DLTLEEKNKI 190 200 SHRGIAFLKF KKFLLKSLFN S

References

[1]

"Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi." Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L., Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B., Smith H.O., Venter J.C. Nature 390:580-586(1997) [PubMed: 9403685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000783 Genomic DNA. Translation: AAB91499.1.
PIR	G70130.
RefSeq	NP_212381.1. NC_001318.1.
3D structure databases
ProteinModelPortal	O51263.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBORT00000008821; EBBORP00000008151; EBBORG00000008820.
GeneID	1195084.
GenomeReviews	Gene locus BB_0247 in contig AE000783_GR.
KEGG	bbu:BB0247.
NMPDR	fig|224326.1.peg.631.
PATRIC	20557110. VBIBorBur75917_0646.
TIGR	BB_0247.
Phylogenomic databases
GeneTree	EBGT00050000007496.
HOGENOM	HBG697237.
OMA	VEDSGFF.
PhylomeDB	O51263.
ProtClustDB	PRK14825.
Enzyme and pathway databases
BioCyc	BBUR224326:BB_0247-MONOMER.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_BORBU

Accession

Primary (citable) accession number: O51263

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	June 1, 1998
Last modified:	January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program