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Protein

Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

Gene

spoT

Organism
Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into GDP. It may also be capable of catalyzing the synthesis of ppGpp (By similarity).By similarity

Catalytic activityi

Guanosine 3',5'-bis(diphosphate) + H2O = guanosine 5'-diphosphate + diphosphate.

Cofactori

Mn2+By similarity

Pathwayi

GO - Molecular functioni

  1. guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. guanosine tetraphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Manganese

Enzyme and pathway databases

BioCyciBBUR224326:G9SO-194-MONOMER.
UniPathwayiUPA00908; UER00886.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC:3.1.7.2)
Alternative name(s):
Penta-phosphate guanosine-3'-pyrophosphohydrolase
Short name:
(ppGpp)ase
Gene namesi
Name:spoT
Ordered Locus Names:BB_0198
OrganismiBorrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Taxonomic identifieri224326 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorreliaBorrelia burgdorferi group
ProteomesiUP000001807: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 667667Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolasePRO_0000166568Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224326.BB0198.

Structurei

3D structure databases

ProteinModelPortaliO51216.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini62 – 161100HDAdd
BLAST

Sequence similaritiesi

Belongs to the RelA/SpoT family.Curated
Contains 1 HD domain.Curated

Phylogenomic databases

eggNOGiCOG0317.
OMAiQIMQVEE.
OrthoDBiEOG6SV551.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PfamiPF01966. HD. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMiSSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00691. spoT_relA. 1 hit.

Sequencei

Sequence statusi: Complete.

O51216-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIQAYEIAHL IKINDLEKAR NIFKKTVENT YKDEFERKSI FKALEIAEQL
60 70 80 90 100
HYGQYRESGE PYIIHPIMVS LFLAKFQLDF KATIAGLLHD VLEDTNVEKE
110 120 130 140 150
EIVKEFDEEI LSLIDGVTKI HDLHNKTRSI KEANTISKMF FAMTHDIRII
160 170 180 190 200
IIKLADKLHN MTTLSYLPKN RQDRIAKDCL STYVPIAERL GISSLKTYLE
210 220 230 240 250
DLSFKHLYPK DYKEIKNFLS ETKIEREKKL YKGKLSIEKE LQKSGIEAEI
260 270 280 290 300
TVRSKHFYSI FRKMQTRTNK LTQIFDTLGI RIICKKQKEC YEILEIVHRV
310 320 330 340 350
WKPIPGRLKD YIASPKENKY QSLHTTVRIP EDNQLIEIQI RTEEMDRIAN
360 370 380 390 400
YGVAAHWIYK EQIELKADDL SFINRIKKWQ QESANKSQYS MNDIHKELLN
410 420 430 440 450
TFIYVYTPEG EVVELPFGSN SIDFAYIIHT DIGDQALYAK INGKISSITK
460 470 480 490 500
PLKNEQIVEI FTSKDSKPDV IWLNSVRTKK ARSKIRSWLN KNDNTIFVDN
510 520 530 540 550
NIIAYLVGAN KEQRKLFSLF KSYTKTKIKR IAIDPECSPT TGEDIIGIIH
560 570 580 590 600
KDEIIVHNEN CQKLKSYKKP QLIEVEWEAT PTRKVHHIIL LLKELKGIFS
610 620 630 640 650
YLENIFTLND VRLISEKIED CGNGHGITNI IVSSNAKNIT KIISALKENP
660
NILQIMQIEE DIKNYDN
Length:667
Mass (Da):77,763
Last modified:June 1, 1998 - v1
Checksum:i608AE939725E2120
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000783 Genomic DNA. Translation: AAC66590.1.
PIRiF70124.
RefSeqiNP_212332.1. NC_001318.1.

Genome annotation databases

EnsemblBacteriaiAAC66590; AAC66590; BB_0198.
GeneIDi1195033.
KEGGibbu:BB_0198.
PATRICi20556998. VBIBorBur75917_0594.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000783 Genomic DNA. Translation: AAC66590.1.
PIRiF70124.
RefSeqiNP_212332.1. NC_001318.1.

3D structure databases

ProteinModelPortaliO51216.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224326.BB0198.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC66590; AAC66590; BB_0198.
GeneIDi1195033.
KEGGibbu:BB_0198.
PATRICi20556998. VBIBorBur75917_0594.

Phylogenomic databases

eggNOGiCOG0317.
OMAiQIMQVEE.
OrthoDBiEOG6SV551.

Enzyme and pathway databases

UniPathwayiUPA00908; UER00886.
BioCyciBBUR224326:G9SO-194-MONOMER.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PfamiPF01966. HD. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMiSSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00691. spoT_relA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.

Entry informationi

Entry nameiSPOT_BORBU
AccessioniPrimary (citable) accession number: O51216
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: January 7, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.