O51128 (SYN_BORBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Asparagine--tRNA ligase
EC=6.1.1.22

Alternative name(s):
Asparaginyl-tRNA synthetase
Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	BB_0101

Organism

Borrelia burgdorferi (Lyme disease spirochete)

Taxonomic identifier

139 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Asparagine--tRNA ligase HAMAP MF_00534		PRO_0000176395

Sequences

	Sequence		Length	Mass (Da)
	O51128 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 2B5900C0E6DBD1F6	FASTA	466	53,600
	10 20 30 40 50 60 MINKMFASIK DILKNPILNS NVTINGWIRT KRSNGKIGFI EINDGSTLKG IQAVINEEEN 70 80 90 100 110 120 QFSEKDLKKL TTGTSISLTG LLVESPAKGQ NYEIKTHSFN VIGETDPETY PLQKKRHSFE 130 140 150 160 170 180 FLREIPHLRI RTNTFGAIAR VRNKISYKIH EYFQKNGFFY INTPIITSND GEGAGEMFRV 190 200 210 220 230 240 STLKFNKLNN ALSNIDFKDD FFGKEAFLSV TGQLHGEAYA MALSKIYTFG PTFRAENSNT 250 260 270 280 290 300 TRHASEFWMI EPEMAFYKLN DNIALAEDLL KYLLSSILNE CSQDMDFLEN YIEKGLIKKL 310 320 330 340 350 360 ENVINSNFEV ITYTKAIEIL ENSKKNFEIK PYWGIDLQTD HERYLTEETF KKPVVVIDYP 370 380 390 400 410 420 KNFKAFYMKA NKDNKTVKGM DILVPKIGEI IGGSEREDDL QKLENRIKEL NLNIEHLNWY 430 440 450 460 LDLRRFGSAP HSGFGLGLER LVQYSTGISN IRDSIPFPRT PKNLYF

References

[1]

"Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi." Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L., Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B., Smith H.O., Venter J.C. Nature 390:580-586(1997) [PubMed: 9403685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000783 Genomic DNA. Translation: AAC66501.1.
PIR	E70112.
RefSeq	NP_212235.1. NC_001318.1.
3D structure databases
ProteinModelPortal	O51128.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBORT00000008535; EBBORP00000007865; EBBORG00000008534.
GeneID	1194937.
GenomeReviews	Gene locus BB_0101 in contig AE000783_GR.
KEGG	bbu:BB0101.
NMPDR	fig|224326.1.peg.485.
PATRIC	20556808. VBIBorBur75917_0499.
TIGR	BB_0101.
Phylogenomic databases
GeneTree	EBGT00050000007238.
HOGENOM	HBG745843.
OMA	AIHRFFH.
PhylomeDB	O51128.
ProtClustDB	PRK03932.
Enzyme and pathway databases
BioCyc	BBUR224326:BB_0101-MONOMER.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01893.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF6. PTHR22594:SF6. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00457. AsnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_BORBU

Accession

Primary (citable) accession number: O51128

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program