O50660 (ALGL_AZOCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alginate lyase
EC=4.2.2.3

Alternative name(s):
Poly(beta-D-mannuronate) lyase
Poly(mana) alginate lyase

Gene names

Name:

algL

Organism

Azotobacter chroococcum mcd 1

Taxonomic identifier

355 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Azotobacter

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Depolymerizes alginate by cleaving the beta-1,4 glycosidic bond. Degrades deacetylated polymannuronate alginate more efficiently than non-deacetylated polyM. Is able to degrade its own alginate, but at a lower efficiency than that produced from M.pyriferia and P.aeruginosa. HAMAP MF_00557
Catalytic activity	Eliminative cleavage of polysaccharides containing beta-D-mannuronate residues to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl groups at their ends. HAMAP MF_00557
Enzyme regulation	Monovalent cations such as potassium and sodium enhance activity, as well as a combined action of these cations with magnesium. However, other cations like calcium, cobalt, manganese and zinc, or the presence of EDTA, do not affect the enzymatic activity. HAMAP MF_00557
Subcellular location	Periplasm HAMAP MF_00557.
Sequence similarities	Belongs to the polysaccharide lyase 5 family.
Biophysicochemical properties	pH dependence: Optimum pH is 7.5. HAMAP MF_00557 Temperature dependence: Optimum temperature is 30 degrees Celsius.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Molecular function	Lyase
Gene Ontology (GO)
Biological process	alginic acid catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	poly(beta-D-mannuronate) lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 372	350	Alginate lyase HAMAP MF_00557		PRO_0000024915

Sequences

	Sequence		Length	Mass (Da)
	O50660 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 23F6D24181038B18	FASTA	372	41,321
	10 20 30 40 50 60 MKTRLALPCL LGSLLLSSAV HAASALVPPK GYYAALEIRK GEAQACQAVP EPYTGELVFR 70 80 90 100 110 120 SKYEGSDSAR STLNKKAEKA FRAKTKPITE IERGVSRMVM RYMEKGRLRR AGMRPGLLDA 130 140 150 160 170 180 WAEDDALLST EYNHTGKSMR KWALGSLAGA YLRLKFSTSQ PLAAYPEQAK RIEAWFAKVG 190 200 210 220 230 240 DQVIKDWSDL PLKQINNHSY WAAWSVMAAG VATNRRPLFD WAVEQFHIAA KQVDPRGFLA 250 260 270 280 290 300 NELKRRQRAL AYHNYSLPPL MMIAAFAQAN GVDLRGDNDG ALGRLAGNVL AGVEDPEPFA 310 320 330 340 350 360 ERAGEDQDME DLETDAKFSW LEPYCALYAC SPALRERKAE MGPFKNFRLG GDVTRIFDPQ 370 EKPSKSTVGN AD

References

[1]

"Cloning and expression of the algL gene, encoding the Azotobacter chroococcum alginate lyase: purification and characterization of the enzyme." Pecina A., Pascual A., Paneque A. J. Bacteriol. 181:1409-1414(1999) [PubMed: 10049370] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 4412 / NCIB 8003 / X-50.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ223605 Genomic DNA. Translation: CAA11481.1.
3D structure databases
ProteinModelPortal	O50660.
ModBase	Search...
Protein family/group databases
CAZy	PL5. Polysaccharide Lyase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	4.2.2.3. 617.
Family and domain databases
HAMAP	MF_00557. Alginate_lyase. [Tree]
InterPro	IPR022859. Alginate_lyase. IPR008397. Alginate_lyase_dom. IPR008929. Chondroitin_lyas. [Graphical view]
Gene3D	G3DSA:1.50.10.110. Alginate_lyase. 1 hit.
Pfam	PF05426. Alginate_lyase. 1 hit. [Graphical view]
SUPFAM	SSF48230. Chondroitin_lyas. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ALGL_AZOCH

Accession

Primary (citable) accession number: O50660

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	September 21, 2011
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program