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Protein

DNA gyrase subunit A

Gene

gyrA

Organism
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.UniRule annotation

Miscellaneous

Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.UniRule annotation

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei123O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA gyrase subunit AUniRule annotation (EC:5.99.1.3UniRule annotation)
Gene namesi
Name:gyrAUniRule annotation
Ordered Locus Names:BH0007
OrganismiBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Taxonomic identifieri272558 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001258 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001452201 – 833DNA gyrase subunit AAdd BLAST833

Interactioni

Subunit structurei

Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.UniRule annotation

Protein-protein interaction databases

STRINGi272558.BH0007.

Structurei

3D structure databases

ProteinModelPortaliO50628.
SMRiO50628.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi525 – 531GyrA-boxUniRule annotation7

Sequence similaritiesi

Belongs to the type II topoisomerase GyrA/ParC subunit family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C24. Bacteria.
COG0188. LUCA.
KOiK02469.
OMAiTHHWLLF.
OrthoDBiPOG091H025U.

Family and domain databases

Gene3Di1.10.268.10. 1 hit.
2.120.10.90. 1 hit.
3.30.1360.40. 1 hit.
3.90.199.10. 1 hit.
HAMAPiMF_01897. GyrA. 1 hit.
InterProiView protein in InterPro
IPR024946. Arg_repress-like_C.
IPR005743. GyrA.
IPR006691. GyrA/parC_rep.
IPR035516. Gyrase/topoIV_suA_C.
IPR013760. Topo_IIA-like_dom_sf.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013757. Topo_IIA_A_a_sf.
PfamiView protein in Pfam
PF03989. DNA_gyraseA_C. 6 hits.
PF00521. DNA_topoisoIV. 1 hit.
SMARTiView protein in SMART
SM00434. TOP4c. 1 hit.
SUPFAMiSSF101904. SSF101904. 1 hit.
SSF56719. SSF56719. 1 hit.

Sequencei

Sequence statusi: Complete.

O50628-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEQDQSRVK EINISQEMKT SFMDYAMSVI VSRALPDVRD GMKPVHRRIL
60 70 80 90 100
YAMNELGMTS DKAYKKSARI VGEVIGKYHP HGDSAVYETM VRMAQDFSYR
110 120 130 140 150
YMLVDGHGNF GSIDGDAAAA MRYTEARMSK ISMELVRDIN KDTIDYQDNY
160 170 180 190 200
DGSEKEPVVM PSRFPNLLVN GASGIAVGMA TNIPPHQLGE VIDGVLALSK
210 220 230 240 250
NPDISVPELM EHIPGPDFPT GAEILGRSGI RKAYQTGRGS ITLRAKTEIE
260 270 280 290 300
EHHGKQRIIV HEIPYQVNKA KLIEKIAELV RDKKIDGITD LRDESDRNGM
310 320 330 340 350
RIVIEVRKDA NANVLLNNLY KQTALQTSFG INLLALVEGQ PKVLNLKECL
360 370 380 390 400
EHYLAHQVIV IRRRTAFELR KAEARAHILE GLRIALDHLD EVISLIRSSQ
410 420 430 440 450
TTEIARNGLM ERFELSYEQA QAILDMRLQR LTGLERDKIE AEYKELIERI
460 470 480 490 500
AELKAILADH EKVLDIIREE LLELKEKYND ERKTAISASE DMFEDEDLIP
510 520 530 540 550
RQNVVITLTH HGYIKRLPIS TYRSQKRGGR GIQGMGTNED DFVQHLFTTN
560 570 580 590 600
SHHTILFFTN KGKVYRLKGY EIPELGRTAK GIPIINLLQI EQDEYISTII
610 620 630 640 650
PIEEFTEDHY LFFMTKDGIA KRTQLSSFAN IRRGGLFAIN LREGDELHGV
660 670 680 690 700
RLTNGDKEVI VGTRQGMAIR FHETDVRLMG RTATGVKGIS LTGDDHVVGM
710 720 730 740 750
DIVEDGQDVL IVTEKGFGKR TPIADYRIQT RGGKGIKTCN ITEKNGPLVS
760 770 780 790 800
LKVVSVDHDL MIITASGIII RLHVKDISVT GRITQGVTLI RVAEGEEVAT
810 820 830
VARVDIEDDE LDEDESIEEE RDDRSEVEQG ENE
Length:833
Mass (Da):93,873
Last modified:June 1, 1998 - v1
Checksum:i176EBB398C7F136B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010081 Genomic DNA. Translation: BAA24188.1.
AB013492 Genomic DNA. Translation: BAA82691.1.
BA000004 Genomic DNA. Translation: BAB03726.1.
PIRiG83650.
T46552.
RefSeqiWP_010896191.1. NC_002570.2.

Genome annotation databases

EnsemblBacteriaiBAB03726; BAB03726; BAB03726.
KEGGibha:BH0007.

Similar proteinsi

Entry informationi

Entry nameiGYRA_BACHD
AccessioniPrimary (citable) accession number: O50628
Secondary accession number(s): Q9JPZ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: November 22, 2017
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families