O50628 (GYRA_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	BH0007

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	833 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Subcellular location	Cytoplasm Potential HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 833	833	DNA gyrase subunit A HAMAP MF_01897		PRO_0000145220
Sites
	Active site	123	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O50628 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 176EBB398C7F136B	FASTA	833	93,873
	10 20 30 40 50 60 MAEQDQSRVK EINISQEMKT SFMDYAMSVI VSRALPDVRD GMKPVHRRIL YAMNELGMTS 70 80 90 100 110 120 DKAYKKSARI VGEVIGKYHP HGDSAVYETM VRMAQDFSYR YMLVDGHGNF GSIDGDAAAA 130 140 150 160 170 180 MRYTEARMSK ISMELVRDIN KDTIDYQDNY DGSEKEPVVM PSRFPNLLVN GASGIAVGMA 190 200 210 220 230 240 TNIPPHQLGE VIDGVLALSK NPDISVPELM EHIPGPDFPT GAEILGRSGI RKAYQTGRGS 250 260 270 280 290 300 ITLRAKTEIE EHHGKQRIIV HEIPYQVNKA KLIEKIAELV RDKKIDGITD LRDESDRNGM 310 320 330 340 350 360 RIVIEVRKDA NANVLLNNLY KQTALQTSFG INLLALVEGQ PKVLNLKECL EHYLAHQVIV 370 380 390 400 410 420 IRRRTAFELR KAEARAHILE GLRIALDHLD EVISLIRSSQ TTEIARNGLM ERFELSYEQA 430 440 450 460 470 480 QAILDMRLQR LTGLERDKIE AEYKELIERI AELKAILADH EKVLDIIREE LLELKEKYND 490 500 510 520 530 540 ERKTAISASE DMFEDEDLIP RQNVVITLTH HGYIKRLPIS TYRSQKRGGR GIQGMGTNED 550 560 570 580 590 600 DFVQHLFTTN SHHTILFFTN KGKVYRLKGY EIPELGRTAK GIPIINLLQI EQDEYISTII 610 620 630 640 650 660 PIEEFTEDHY LFFMTKDGIA KRTQLSSFAN IRRGGLFAIN LREGDELHGV RLTNGDKEVI 670 680 690 700 710 720 VGTRQGMAIR FHETDVRLMG RTATGVKGIS LTGDDHVVGM DIVEDGQDVL IVTEKGFGKR 730 740 750 760 770 780 TPIADYRIQT RGGKGIKTCN ITEKNGPLVS LKVVSVDHDL MIITASGIII RLHVKDISVT 790 800 810 820 830 GRITQGVTLI RVAEGEEVAT VARVDIEDDE LDEDESIEEE RDDRSEVEQG ENE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of the genes encoding DNA gyrase from alkaliphilic Bacillus sp. strain C-125." Masui N., Nakasone K., Horikoshi K. Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.
[2]	"Replication origin region of the chromosome of alkaliphilic Bacillus halodurans C-125." Takami H., Masui N., Nakasone K., Horikoshi K. Biosci. Biotechnol. Biochem. 63:1134-1137(1999) [PubMed: 10427704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.
[3]	"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB010081 Genomic DNA. Translation: BAA24188.1. AB013492 Genomic DNA. Translation: BAA82691.1. BA000004 Genomic DNA. Translation: BAB03726.1.
PIR	G83650. T46552.
RefSeq	NP_240873.1. NC_002570.2.
3D structure databases
ProteinModelPortal	O50628.
SMR	O50628. Positions 31-487.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000051460; EBBACP00000050099; EBBACG00000051451.
GeneID	890514.
GenomeReviews	Gene locus BH0007 in contig BA000004_GR.
KEGG	bha:BH0007.
NMPDR	fig|272558.1.peg.7.
PATRIC	18936820. VBIBacHal18977_0007.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000002933.
HOGENOM	HBG635183.
OMA	TGRGRIY.
PhylomeDB	O50628.
ProtClustDB	PRK05560.
Enzyme and pathway databases
BioCyc	BHAL272558:BH0007-MONOMER.
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR010235. NucleotidylTrfase-bd_HI0074. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
KO	K02469.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. PF08780. NTase_sub_bind. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_BACHD

Accession

Primary (citable) accession number: O50628
Secondary accession number(s): Q9JPZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program