O50533 (HEMH_STRCO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable ferrochelatase
EC=4.99.1.1

Alternative name(s):
Heme synthase
Protoheme ferro-lyase

Gene names

Name:	hemH
Ordered Locus Names:	SCO5859
ORF Names:	SC9B10.26

Organism

Streptomyces coelicolor

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity HAMAP MF_00323.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	Probable ferrochelatase HAMAP MF_00323		PRO_0000175210
Sites
	Metal binding	191	1	Iron By similarity
	Metal binding	286	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	O50533 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 650547197DF326FD	FASTA	375	40,622
	10 20 30 40 50 60 MPDVLDASPY DALLLLSFGG PEGPDDVVPF LENVTRGRGI PKERLKEVGQ HYFLFGGVSP 70 80 90 100 110 120 INDQNRALLD ALRKDFAEHG LDLPVYWGNR NWAPYLTDTL REMVGDGRRR ILVLATSAYA 130 140 150 160 170 180 SYSGCRQYRE NLADALAALE SEGLELPKID KLRHYFNHPG FVEPMVDGVV RSLAELPAEV 190 200 210 220 230 240 RDGAHIAFCT HSIPTSAADG SGPVEEHGDG GAYVRQHLDV ARLIADAVRE RTGVDHPWQL 250 260 270 280 290 300 VYQSRSGAPH IPWLEPDICD HLEERQAAGV PAVVMAPIGF VSDHMEVLYD LDTEATAKAE 310 320 330 340 350 360 ELGLPVRRSA TVGADPRFAA AVRDLVLERA GDERGQEVTP CALGTLGASH NLCPVGCCPA 370 RAPRPAAAGA DSPYA

References

[1]

"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL939125 Genomic DNA. Translation: CAA15816.1.
PIR	T35895.
RefSeq	NP_629982.1. NC_003888.3.
3D structure databases
ProteinModelPortal	O50533.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1101301.
GenomeReviews	Gene locus SCO5859 in contig AL645882_GR.
KEGG	sco:SCO5859.
NMPDR	fig|100226.1.peg.5808.
PATRIC	23741622. VBIStrCoe124346_5958.
Phylogenomic databases
HOGENOM	HBG697135.
OMA	PAVVMAP.
PhylomeDB	O50533.
ProtClustDB	PRK00035.
Family and domain databases
HAMAP	MF_00323. Ferrochelatase. [Tree]
InterPro	IPR001015. Ferrochelatase. [Graphical view]
KO	K01772.
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00109. HemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_STRCO

Accession

Primary (citable) accession number: O50533

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program