O50519 (KITH_STRCO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:	tdk
Ordered Locus Names:	SCO5845
ORF Names:	SC9B10.12

Organism

Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) [Reference proteome] [HAMAP]

Taxonomic identifier

100226 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces › Streptomyces coelicolor

Protein attributes

Sequence length	216 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate. HAMAP-Rule MF_00124
Subunit structure	Homotetramer By similarity. HAMAP-Rule MF_00124
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00124.
Sequence similarities	Belongs to the thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP thymidine kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 216	216	Thymidine kinase HAMAP-Rule MF_00124		PRO_0000175029
Regions
	Nucleotide binding	9 – 16	8	ATP By similarity
	Nucleotide binding	86 – 89	4	ATP By similarity
Sites
	Active site	87	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	O50519 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: A04BA1CE8C3B4ADA	FASTA	216	23,368
	10 20 30 40 50 60 MPELVFFSGT MDCGKSTLAL QIEHNRSARG LAGMIFTRDD RAGEGKLSSR LGLVTDAVEV 70 80 90 100 110 120 GDGQDLYAHV VDHLSQGGRV DYVIADEAQF LAPDQIDQLA RVVDDLDVDV YAFGITTDFR 130 140 150 160 170 180 SKLFPGSQRL VELADRVEVL QVEALCWCGA RATHNARTVG GVMVVEGAQV VVGDVAQSPD 190 200 210 EIGYEVLCRR HHRRRMTSAT ARAAALSPDV LPVTAT

References

[1]

"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL939125 Genomic DNA. Translation: CAA15802.1.
PIR	T35881.
RefSeq	NP_629968.1. NC_003888.3.
3D structure databases
ProteinModelPortal	O50519.
ModBase	Search...
Protein-protein interaction databases
STRING	100226.SCO5845.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAA15802; CAA15802; CAA15802.
GeneID	1101287.
KEGG	sco:SCO5845.
PATRIC	23741592. VBIStrCoe124346_5943.
Phylogenomic databases
eggNOG	COG1435.
HOGENOM	HOG000076391.
KO	K00857.
OMA	FRYGAMN.
ProtClustDB	PRK04296.
Enzyme and pathway databases
BRENDA	2.7.1.21. 5998.
Family and domain databases
HAMAP	MF_00124. Thymidine_kinase.
InterPro	IPR027417. P-loop_NTPase. IPR001267. Thymidine_kinase. IPR020634. Thymidine_kinase_subgr. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
SUPFAM	SSF52540. SSF52540. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_STRCO

Accession

Primary (citable) accession number: O50519

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	June 1, 1998
Last modified:	May 29, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program