Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Type B diterpene cyclase

Gene

Rv3377c

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of tuberculosinyl diphosphate from geranylgeranyl diphosphate (GGPP). It could also react with (14R/S)-14,15-oxidoGGPP to generate 3alpha- and 3beta-hydroxytuberculosinyl diphosphate.2 Publications

Catalytic activityi

Geranylgeranyl diphosphate = tuberculosinyl diphosphate.2 Publications

Cofactori

Mg2+1 Publication

Enzyme regulationi

Strongly inhibited by 15-aza-dihydrogeranylgeraniol and 5-isopropyl-N,N,N,2-tetramethyl-4-(piperidine-1-carbonyloxy)benzenaminium chloride (Amo-1618). Inhibited by GGPP concentrations higher than 50 µM.1 Publication

Kineticsi

  1. KM=11.7 µM for GGPP (at 30 degrees Celsius and pH 7.5)1 Publication
  1. Vmax=186 nmol/min/mg enzyme (at 30 degrees Celsius and pH 7.5)1 Publication

pH dependencei

Optimum pH is 7.5. Enzyme activity is not detected at pH values above 9.0.1 Publication

Temperature dependencei

Optimum temperature is 30 degrees Celsius.1 Publication

GO - Molecular functioni

  • halimadienyl-diphosphate synthase activity Source: MTBBASE
  • lyase activity Source: UniProtKB-KW
  • magnesium ion binding Source: MTBBASE

GO - Biological processi

  • cellular response to magnesium starvation Source: MTBBASE
  • geranylgeranyl diphosphate metabolic process Source: MTBBASE
  • response to host immune response Source: MTBBASE
  • tuberculosinol biosynthetic process Source: MTBBASE
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13989.
MTBRV:RV3377C-MONOMER.

Chemistry

SwissLipidsiSLP:000001170.

Names & Taxonomyi

Protein namesi
Recommended name:
Type B diterpene cyclase (EC:5.5.1.16)
Gene namesi
Ordered Locus Names:Rv3377c
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv3377c.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi292 – 2921E → Q: No change. 1 Publication
Mutagenesisi293 – 2931D → E: Loss of cyclase activity. 1 Publication
Mutagenesisi293 – 2931D → N: Loss of cyclase activity. 1 Publication
Mutagenesisi295 – 2951D → N: Loss of cyclase activity. 1 Publication
Mutagenesisi295 – 2951D → Q: Loss of cyclase activity. 1 Publication
Mutagenesisi296 – 2961T → D: Loss of cyclase activity. 1 Publication
Mutagenesisi296 – 2961T → N: Loss of cyclase activity. 1 Publication
Mutagenesisi296 – 2961T → S: Loss of cyclase activity. 1 Publication
Mutagenesisi296 – 2961T → V: Loss of cyclase activity. 1 Publication
Mutagenesisi297 – 2971T → S: Loss of cyclase activity. 1 Publication
Mutagenesisi297 – 2971T → V: Loss of cyclase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 501501Type B diterpene cyclasePRO_0000416457Add
BLAST

Proteomic databases

PaxDbiO50406.
PRIDEiO50406.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi83332.Rv3377c.

Structurei

3D structure databases

ProteinModelPortaliO50406.
SMRiO50406. Positions 5-324, 401-470.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the terpene synthase family.Curated

Phylogenomic databases

eggNOGiENOG4107R2E. Bacteria.
ENOG410XP7U. LUCA.
HOGENOMiHOG000220626.
InParanoidiO50406.
KOiK17811.
OMAiELYCPTR.

Family and domain databases

Gene3Di1.50.10.20. 2 hits.
InterProiIPR001330. PFTB_repeat.
IPR032696. SQ_cyclase_C.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF00432. Prenyltrans. 1 hit.
PF13243. SQHop_cyclase_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.

Sequencei

Sequence statusi: Complete.

O50406-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METFRTLLAK AALGNGISST AYDTAWVAKL GQLDDELSDL ALNWLCERQL
60 70 80 90 100
PDGSWGAEFP FCYEDRLLST LAAMISLTSN KHRRRRAAQV EKGLLALKNL
110 120 130 140 150
TSGAFEGPQL DIKDATVGFE LIAPTLMAEA ARLGLAICHE ESILGELVGV
160 170 180 190 200
REQKLRKLGG SKINKHITAA FSVELAGQDG VGMLDVDNLQ ETNGSVKYSP
210 220 230 240 250
SASAYFALHV KPGDKRALAY ISSIIQAGDG GAPAFYQAEI FEIVWSLWNL
260 270 280 290 300
SRTDIDLSDP EIVRTYLPYL DHVEQHWVRG RGVGWTGNST LEDCDTTSVA
310 320 330 340 350
YDVLSKFGRS PDIGAVLQFE DADWFRTYFH EVGPSISTNV HVLGALKQAG
360 370 380 390 400
YDKCHPRVRK VLEFIRSSKE PGRFCWRDKW HRSAYYTTAH LICAASNYDD
410 420 430 440 450
ALCSDAIGWI LNTQRPDGSW GFFDGQATAE ETAYCIQALA HWQRHSGTSL
460 470 480 490 500
SAQISRAGGW LSQHCEPPYA PLWIAKTLYC SATVVKAAIL SALRLVDESN

Q
Length:501
Mass (Da):55,262
Last modified:June 1, 1998 - v1
Checksum:iB9B6076F13837AFE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP46198.1.
PIRiH70972.
RefSeqiNP_217894.1. NC_000962.3.
WP_003417905.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP46198; CCP46198; Rv3377c.
GeneIDi888073.
KEGGimtu:Rv3377c.
PATRICi18156124. VBIMycTub87468_3772.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP46198.1.
PIRiH70972.
RefSeqiNP_217894.1. NC_000962.3.
WP_003417905.1. NZ_KK339370.1.

3D structure databases

ProteinModelPortaliO50406.
SMRiO50406. Positions 5-324, 401-470.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv3377c.

Chemistry

SwissLipidsiSLP:000001170.

Proteomic databases

PaxDbiO50406.
PRIDEiO50406.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP46198; CCP46198; Rv3377c.
GeneIDi888073.
KEGGimtu:Rv3377c.
PATRICi18156124. VBIMycTub87468_3772.

Organism-specific databases

TubercuListiRv3377c.

Phylogenomic databases

eggNOGiENOG4107R2E. Bacteria.
ENOG410XP7U. LUCA.
HOGENOMiHOG000220626.
InParanoidiO50406.
KOiK17811.
OMAiELYCPTR.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13989.
MTBRV:RV3377C-MONOMER.

Family and domain databases

Gene3Di1.50.10.20. 2 hits.
InterProiIPR001330. PFTB_repeat.
IPR032696. SQ_cyclase_C.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF00432. Prenyltrans. 1 hit.
PF13243. SQHop_cyclase_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiDITCY_MYCTU
AccessioniPrimary (citable) accession number: O50406
Secondary accession number(s): F2GEC3, L0TCC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2012
Last sequence update: June 1, 1998
Last modified: September 7, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.