O50399 (DNAE2_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Error-prone DNA polymerase
EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	Rv3370c, MT3480

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	1098 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase. Does not appear to be essential for chromosomal replication. May be involved in generating antibiotic resistance. Ref.4
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm By similarity HAMAP MF_01902.
Induction	Up-regulated in response to all forms of DNA damage. LexA-regulated. Ref.3
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.
Sequence caution	The sequence CAA15755.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW response to antibiotic Inferred from expression pattern. Source: MTBBASE
Cellular component	cell wall Inferred from direct assay. Source: MTBBASE cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: MTBBASE
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1098	1098	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103385

Sequences

	Sequence		Length	Mass (Da)
	O50399 [UniParc]. Last modified July 5, 2005. Version 4. Checksum: F07709ACDBF35E88	FASTA	1,098	118,672
	10 20 30 40 50 60 MFDILWNVGW SNGPPSWAEM ERVLNGKPRH AGVPAFDADG DVPRSRKRGA YQPPGRERVG 70 80 90 100 110 120 SSVAYAELHA HSAYSFLDGA STPEELVEEA ARLGLCALAL TDHDGLYGAV RFAEAAAELD 130 140 150 160 170 180 VRTVFGAELS LGATARTERP DPPGPHLLVL ARGPEGYRRL SRQLAAAHLA GGEKGKPRYD 190 200 210 220 230 240 FDALTEAAGG HWHILTGCRK GHVRQALSQG GPAAAQRALA DLVDRFTPSR VSIELTHHGH 250 260 270 280 290 300 PLDDERNAAL AGLAPRFGVG IVATTGAHFA DPSRGRLAMA MAAIRARRSL DSAAGWLAPL 310 320 330 340 350 360 GGAHLRSGEE MARLFAWCPE AVTAAAELGE RCAFGLQLIA PRLPPFDVPD GHTEDSWLRS 370 380 390 400 410 420 LVMAGARERY GPPKSAPRAY SQIEHELKVI AQLRFPGYFL VVHDITRFCR DNDILCQGRG 430 440 450 460 470 480 SAANSAVCYA LGVTAVDPVA NELLFERFLS PARDGPPDID IDIESDQREK VIQYVYHKYG 490 500 510 520 530 540 RDYAAQVANV ITYRGRSAVR DMARALGFSP GQQDAWSKQV SHWTGQADDV DGIPEQVIDL 550 560 570 580 590 600 ATQIRNLPRH LGIHSGGMVI CDRPIADVCP VEWARMANRS VLQWDKDDCA AIGLVKFDLL 610 620 630 640 650 660 GLGMLSALHY AKDLVAEHKG IEVDLARLDL SEPAVYEMLA RADSVGVFQV ESRAQMATLP 670 680 690 700 710 720 RLKPRVFYDL VVEVALIRPG PIQGGSVHPY IRRRNGVDPV IYEHPSMAPA LRKTLGVPLF 730 740 750 760 770 780 QEQLMQLAVD CAGFSAAEAD QLRRAMGSKR STERMRRLRG RFYDGMRALH GAPDEVIDRI 790 800 810 820 830 840 YEKLEAFANF GFPESHALSF ASLVFYSAWF KLHHPAAFCA ALLRAQPMGF YSPQSLVADA 850 860 870 880 890 900 RRHGVAVHGP CVNASLAHAT CENAGTEVRL GLGAVRYLGA ELAEKLVAER TANGPFTSLP 910 920 930 940 950 960 DLTSRVQLSV PQVEALATAG ALGCFGMSRR EALWAAGAAA TGRPDRLPGV GSSSHIPALP 970 980 990 1000 1010 1020 GMSELELAAA DVWATGVSPD SYPTQFLRAD LDAMGVLPAE RLGSVSDGDR VLIAGAVTHR 1030 1040 1050 1060 1070 1080 QRPATAQGVT FINLEDETGM VNVLCTPGVW ARHRKLAHTA PALLIRGQVQ NASGAITVVA 1090 ERMGRLTLAV GARSRDFR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"Definition of the mycobacterial SOS box and use to identify LexA-regulated genes in Mycobacterium tuberculosis." Davis E.O., Dullaghan E.M., Rand L. J. Bacteriol. 184:3287-3295(2002) [PubMed: 12029045] [Abstract] Cited for: INDUCTION BY LEXA. Strain: ATCC 25618 / H37Rv.
[4]	"DnaE2 polymerase contributes to in vivo survival and the emergence of drug resistance in Mycobacterium tuberculosis." Boshoff H.I.M., Reed M.B., Barry C.E. III, Mizrahi V. Cell 113:183-193(2003) [PubMed: 12705867] [Abstract] Cited for: FUNCTION. Strain: ATCC 25618 / H37Rv.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842582 Genomic DNA. Translation: CAA15755.1. Different initiation. AE000516 Genomic DNA. Translation: AAK47817.1.
PIR	A70972.
RefSeq	NP_338003.2. NC_002755.2.
3D structure databases
ProteinModelPortal	O50399.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000000506; EBMYCP00000000506; EBMYCG00000000506. EBMYCT00000071896; EBMYCP00000069955; EBMYCG00000071891.
GeneID	926513.
GenomeReviews	Gene locus MT3480 in contig AE000516_GR. Gene locus Rv3370c in contig AL123456_GR.
KEGG	mtc:MT3480. mtu:Rv3370c.
PATRIC	18129394. VBIMycTub22151_3793.
TIGR	MT3480.
Organism-specific databases
TubercuList	Rv3370c.
Phylogenomic databases
GeneTree	EBGT00050000016568.
HOGENOM	HBG734490.
OMA	EQPAIRL.
PhylomeDB	O50399.
ProtClustDB	PRK05672.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR023073. DNA_pol_error_prone. IPR004365. NA-bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR016195. Pol/histidinol_Pase-like. IPR004805. PolC_alpha. [Graphical view]
KO	K14162.
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
SUPFAM	SSF89550. PHP-like. 1 hit.
TIGRFAMs	TIGR00594. Polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_MYCTU

Accession

Primary (citable) accession number: O50399
Secondary accession number(s): Q7D5L9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	July 5, 2005
Last modified:	January 25, 2012
This is version 78 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program