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O50399 (DNAE2_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Error-prone DNA polymerase

EC=2.7.7.7
Gene names
Name:dnaE2
Ordered Locus Names:Rv3370c, MT3480
OrganismMycobacterium tuberculosis
Taxonomic identifier1773 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

Protein attributes

Sequence length1098 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase. Does not appear to be essential for chromosomal replication. May be involved in generating antibiotic resistance. Ref.4

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902

Subcellular location

Cytoplasm By similarity HAMAP MF_01902.

Induction

Up-regulated in response to all forms of DNA damage. LexA-regulated. Ref.3

Sequence similarities

Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Sequence caution

The sequence CAA15755.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10981098Error-prone DNA polymerase HAMAP MF_01902
PRO_0000103385

Sequences

Sequence LengthMass (Da)Tools
O50399 [UniParc].

Last modified July 5, 2005. Version 4.
Checksum: F07709ACDBF35E88

FASTA1,098118,672
        10         20         30         40         50         60 
MFDILWNVGW SNGPPSWAEM ERVLNGKPRH AGVPAFDADG DVPRSRKRGA YQPPGRERVG 

        70         80         90        100        110        120 
SSVAYAELHA HSAYSFLDGA STPEELVEEA ARLGLCALAL TDHDGLYGAV RFAEAAAELD 

       130        140        150        160        170        180 
VRTVFGAELS LGATARTERP DPPGPHLLVL ARGPEGYRRL SRQLAAAHLA GGEKGKPRYD 

       190        200        210        220        230        240 
FDALTEAAGG HWHILTGCRK GHVRQALSQG GPAAAQRALA DLVDRFTPSR VSIELTHHGH 

       250        260        270        280        290        300 
PLDDERNAAL AGLAPRFGVG IVATTGAHFA DPSRGRLAMA MAAIRARRSL DSAAGWLAPL 

       310        320        330        340        350        360 
GGAHLRSGEE MARLFAWCPE AVTAAAELGE RCAFGLQLIA PRLPPFDVPD GHTEDSWLRS 

       370        380        390        400        410        420 
LVMAGARERY GPPKSAPRAY SQIEHELKVI AQLRFPGYFL VVHDITRFCR DNDILCQGRG 

       430        440        450        460        470        480 
SAANSAVCYA LGVTAVDPVA NELLFERFLS PARDGPPDID IDIESDQREK VIQYVYHKYG 

       490        500        510        520        530        540 
RDYAAQVANV ITYRGRSAVR DMARALGFSP GQQDAWSKQV SHWTGQADDV DGIPEQVIDL 

       550        560        570        580        590        600 
ATQIRNLPRH LGIHSGGMVI CDRPIADVCP VEWARMANRS VLQWDKDDCA AIGLVKFDLL 

       610        620        630        640        650        660 
GLGMLSALHY AKDLVAEHKG IEVDLARLDL SEPAVYEMLA RADSVGVFQV ESRAQMATLP 

       670        680        690        700        710        720 
RLKPRVFYDL VVEVALIRPG PIQGGSVHPY IRRRNGVDPV IYEHPSMAPA LRKTLGVPLF 

       730        740        750        760        770        780 
QEQLMQLAVD CAGFSAAEAD QLRRAMGSKR STERMRRLRG RFYDGMRALH GAPDEVIDRI 

       790        800        810        820        830        840 
YEKLEAFANF GFPESHALSF ASLVFYSAWF KLHHPAAFCA ALLRAQPMGF YSPQSLVADA 

       850        860        870        880        890        900 
RRHGVAVHGP CVNASLAHAT CENAGTEVRL GLGAVRYLGA ELAEKLVAER TANGPFTSLP 

       910        920        930        940        950        960 
DLTSRVQLSV PQVEALATAG ALGCFGMSRR EALWAAGAAA TGRPDRLPGV GSSSHIPALP 

       970        980        990       1000       1010       1020 
GMSELELAAA DVWATGVSPD SYPTQFLRAD LDAMGVLPAE RLGSVSDGDR VLIAGAVTHR 

      1030       1040       1050       1060       1070       1080 
QRPATAQGVT FINLEDETGM VNVLCTPGVW ARHRKLAHTA PALLIRGQVQ NASGAITVVA 

      1090 
ERMGRLTLAV GARSRDFR 

« Hide

References

« Hide 'large scale' references
[1]"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence."
Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. expand/collapse author list , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G.
Nature 393:537-544(1998) [PubMed: 9634230] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25618 / H37Rv.
[2]"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains."
Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. expand/collapse author list , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.
J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CDC 1551 / Oshkosh.
[3]"Definition of the mycobacterial SOS box and use to identify LexA-regulated genes in Mycobacterium tuberculosis."
Davis E.O., Dullaghan E.M., Rand L.
J. Bacteriol. 184:3287-3295(2002) [PubMed: 12029045] [Abstract]
Cited for: INDUCTION BY LEXA.
Strain: ATCC 25618 / H37Rv.
[4]"DnaE2 polymerase contributes to in vivo survival and the emergence of drug resistance in Mycobacterium tuberculosis."
Boshoff H.I.M., Reed M.B., Barry C.E. III, Mizrahi V.
Cell 113:183-193(2003) [PubMed: 12705867] [Abstract]
Cited for: FUNCTION.
Strain: ATCC 25618 / H37Rv.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX842582 Genomic DNA. Translation: CAA15755.1. Different initiation.
AE000516 Genomic DNA. Translation: AAK47817.1.
PIRA70972.
RefSeqNP_338003.2. NC_002755.2.

3D structure databases

ProteinModelPortalO50399.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000000506; EBMYCP00000000506; EBMYCG00000000506.
EBMYCT00000071896; EBMYCP00000069955; EBMYCG00000071891.
GeneID926513.
GenomeReviewsGene locus MT3480 in contig AE000516_GR.
Gene locus Rv3370c in contig AL123456_GR.
KEGGmtc:MT3480.
mtu:Rv3370c.
PATRIC18129394. VBIMycTub22151_3793.
TIGRMT3480.

Organism-specific databases

TubercuListRv3370c.

Phylogenomic databases

GeneTreeEBGT00050000016568.
HOGENOMHBG734490.
OMAEQPAIRL.
PhylomeDBO50399.
ProtClustDBPRK05672.

Family and domain databases

HAMAPMF_01902. DNApol_error_prone.
[Tree]
InterProIPR011708. DNA_pol3_alpha.
IPR023073. DNA_pol_error_prone.
IPR004365. NA-bd_OB_tRNA-helicase.
IPR004013. PHP_C.
IPR003141. Pol/His_phosphatase_N.
IPR016195. Pol/histidinol_Pase-like.
IPR004805. PolC_alpha.
[Graphical view]
KOK14162.
PfamPF07733. DNA_pol3_alpha. 1 hit.
PF02811. PHP. 1 hit.
PF01336. tRNA_anti. 1 hit.
[Graphical view]
SMARTSM00481. POLIIIAc. 1 hit.
[Graphical view]
SUPFAMSSF89550. PHP-like. 1 hit.
TIGRFAMsTIGR00594. Polc. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDNAE2_MYCTU
AccessionPrimary (citable) accession number: O50399
Secondary accession number(s): Q7D5L9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2005
Last modified: January 25, 2012
This is version 78 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families