Endo-pectate lyase precursor - Erwinia chrysanthemi

Preferred order of sections

Names and origin

Protein names

Submitted name:
Endo-pectate lyase EMBL CAA73784.1
EC=4.2.2.2 EMBL CAA73784.1

Gene names

Name:

pelI EMBL CAA73784.1

Organism

Erwinia chrysanthemi EMBL CAA73784.1

Taxonomic identifier

556 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Dickeya

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

Ontologies

Keywords
Domain	Signal EMBL CAA73784.1
Ligand	Calcium PDB 3B4N PDB 3B90 Metal-binding PDB 3B4N PDB 3B90 Zinc PDB 3B4N PDB 3B90
Molecular function	Lyase EMBL CAA73784.1
Technical term	3D-structure PDB 3B4N PDB 3B90 PDB 3B8Y
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: InterPro
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Potential EMBL CAA73784.1

Chain

20 – 344

325

Potential EMBL CAA73784.1

PRO_5000147160

Sites

Metal binding

176

1

Zinc; via pros nitrogen PDB 3B4N PDB 3B90

Metal binding

178

1

Zinc PDB 3B4N PDB 3B90

Metal binding

191

1

Calcium PDB 3B4N PDB 3B90

Metal binding

192

1

Calcium; via carbonyl oxygen PDB 3B4N PDB 3B90

Metal binding

213

1

Calcium PDB 3B4N PDB 3B90

Metal binding

218

1

Calcium; via carbonyl oxygen PDB 3B4N PDB 3B90

Sequences

Sequence

Length

Mass (Da)

Tools

O50325 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 2812B3652439FA76
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FASTA

344

36,851

        10         20         30         40         50         60 
MFKYVIPLCA LTLAAPSFAA QTTLMLSQKS DVNYLGWSTD ESKVARQEVY RGTTSNPDLR 

        70         80         90        100        110        120 
ERIAVLDAET RTFKDADTNS GLNYWYWVDV VSENQAQVVS NAVTTAPNAG PLRAAKASSE 

       130        140        150        160        170        180 
CKPGATFENR TVDCGGVTIG TSCPNDSDKQ KPLIILKNAT VKNLRISASG RADGIHCDSG 

       190        200        210        220        230        240 
NCTIENVIWE DICEDAATNN GKTMTIVGGI AHNAKDGYGG KPDKVLQHNS KNSTTVVKGN 

       250        260        270        280        290        300 
FTLTGEHGKL WRSCGDCSNN GGPRFLTVTS ATVNGTIDSI AGVNRNYGDV ATISGLKIKN 

       310        320        330        340 
YKEGKPPVCE EFKGVVKGQG STEKYGEKWD TTNCKVSRSG VSKL

« Hide

References

[1]	"Pectate lyase PelI of Erwinia chrysanthemi 3937 belongs to a new family." Shevchik V.E., Robert-Baudouy J., Hugouvieux-Cotte-Pattat N. J. Bacteriol. 179:7321-7330(1997) [PubMed: 9393696] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: 3937 EMBL CAA73784.1.
[2]	"The crystal structure of pectate lyase peli from soft rot pathogen Erwinia chrysanthemi in complex with its substrate." Creze C., Castang S., Derivery E., Haser R., Hugouvieux-Cotte-Pattat N., Shevchik V.E., Gouet P. J. Biol. Chem. 283:18260-18268(2008) [PubMed: 18430740] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) IN COMPLEX WITH CALCIUM AND ZINC.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Y13340 Genomic DNA. Translation: CAA73784.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3B4N	X-ray	1.45	A/B	1-344	[»]
3B8Y	X-ray	2.30	A/B	1-344	[»]
3B90	X-ray	2.11	A/B	119-344	[»]

ProteinModelPortal

O50325.

SMR

O50325. Positions 20-344.

ModBase

Search...

Protein family/group databases

CAZy

PL3. Polysaccharide Lyase Family 3.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR013783. Ig-like_fold.
IPR004898. Pectate_lyase_cat.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]

Gene3D

G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.

Pfam

PF03211. Pectate_lyase. 1 hit.
[Graphical view]

SUPFAM

SSF51126. Pectin_lyas_like. 1 hit.

ProtoNet

Search...

Entry information

Entry name

O50325_ERWCH

Accession

Primary (citable) accession number: O50325

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 1998
Last sequence update:	June 1, 1998
Last modified:	February 8, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)