Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein
Submitted name:

Endo-pectate lyase

Gene

pelI

Organism
Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi173Zinc 1Combined sources1
Metal bindingi176Zinc 2; via pros nitrogenCombined sources1
Metal bindingi178Zinc 2Combined sources1
Metal bindingi191CalciumCombined sources1
Metal bindingi192Calcium; via carbonyl oxygenCombined sources1
Metal bindingi195Zinc 1Combined sources1
Binding sitei195Galacturonate 1Combined sources1
Metal bindingi213CalciumCombined sources1
Metal bindingi218Calcium; via carbonyl oxygenCombined sources1
Binding sitei227Galacturonate 2Combined sources1
Binding sitei256Galacturonate 1Combined sources1
Binding sitei258Galacturonate 4Combined sources1
Binding sitei317Galacturonate 1Combined sources1
Binding sitei322Galacturonate 3Combined sources1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

LyaseImported

Keywords - Ligandi

CalciumCombined sources, Metal-bindingCombined sources, ZincCombined sources

Enzyme and pathway databases

BRENDAi4.2.2.2. 2141.

Protein family/group databases

CAZyiPL3. Polysaccharide Lyase Family 3.

Names & Taxonomyi

Protein namesi
Submitted name:
Endo-pectate lyaseImported (EC:4.2.2.2Imported)
Gene namesi
Name:pelIImported
OrganismiDickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi)Imported
Taxonomic identifieri556 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesPectobacteriaceaeDickeya

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_500415886020 – 344Sequence analysisAdd BLAST325

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi121 ↔ 134Combined sources
Disulfide bondi143 ↔ 193Combined sources
Disulfide bondi177 ↔ 182Combined sources
Disulfide bondi254 ↔ 257Combined sources
Disulfide bondi309 ↔ 334Combined sources

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3B4NX-ray1.45A/B1-344[»]
3B8YX-ray2.30A/B1-344[»]
3B90X-ray2.11A/B119-344[»]
ProteinModelPortaliO50325.
SMRiO50325.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO50325.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini114 – 318Pectate_lyaseInterPro annotationAdd BLAST205

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni227 – 229Galacturonate 1 bindingCombined sources3
Regioni249 – 252Galacturonate 3 bindingCombined sources4
Regioni255 – 256Galacturonate 2 bindingCombined sources2

Keywords - Domaini

SignalSequence analysis

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
2.60.40.10. 1 hit.
InterProiIPR013783. Ig-like_fold.
IPR004898. Pectate_lyase_cat.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF03211. Pectate_lyase. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O50325-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKYVIPLCA LTLAAPSFAA QTTLMLSQKS DVNYLGWSTD ESKVARQEVY
60 70 80 90 100
RGTTSNPDLR ERIAVLDAET RTFKDADTNS GLNYWYWVDV VSENQAQVVS
110 120 130 140 150
NAVTTAPNAG PLRAAKASSE CKPGATFENR TVDCGGVTIG TSCPNDSDKQ
160 170 180 190 200
KPLIILKNAT VKNLRISASG RADGIHCDSG NCTIENVIWE DICEDAATNN
210 220 230 240 250
GKTMTIVGGI AHNAKDGYGG KPDKVLQHNS KNSTTVVKGN FTLTGEHGKL
260 270 280 290 300
WRSCGDCSNN GGPRFLTVTS ATVNGTIDSI AGVNRNYGDV ATISGLKIKN
310 320 330 340
YKEGKPPVCE EFKGVVKGQG STEKYGEKWD TTNCKVSRSG VSKL
Length:344
Mass (Da):36,851
Last modified:June 1, 1998 - v1
Checksum:i2812B3652439FA76
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13340 Genomic DNA. Translation: CAA73784.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13340 Genomic DNA. Translation: CAA73784.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3B4NX-ray1.45A/B1-344[»]
3B8YX-ray2.30A/B1-344[»]
3B90X-ray2.11A/B119-344[»]
ProteinModelPortaliO50325.
SMRiO50325.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiPL3. Polysaccharide Lyase Family 3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi4.2.2.2. 2141.

Miscellaneous databases

EvolutionaryTraceiO50325.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
2.60.40.10. 1 hit.
InterProiIPR013783. Ig-like_fold.
IPR004898. Pectate_lyase_cat.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF03211. Pectate_lyase. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiO50325_DICCH
AccessioniPrimary (citable) accession number: O50325
Entry historyi
Integrated into UniProtKB/TrEMBL: June 1, 1998
Last sequence update: June 1, 1998
Last modified: November 2, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.