Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O50316

- IMDH_CHLP8

UniProt

O50316 - IMDH_CHLP8

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Inosine-5'-monophosphate dehydrogenase

Gene

guaB

Organism
Chlorobaculum parvum (strain NCIB 8327) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 263 / NCIB 8327))
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.UniRule annotation

Catalytic activityi

Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

Cofactori

K(+)UniRule annotation

Enzyme regulationi

Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei251 – 2511NADUniRule annotation
Metal bindingi303 – 3031Potassium; via carbonyl oxygenUniRule annotation
Metal bindingi305 – 3051Potassium; via carbonyl oxygenUniRule annotation
Binding sitei306 – 3061IMPUniRule annotation
Active sitei308 – 3081Thioimidate intermediateUniRule annotation
Metal bindingi308 – 3081Potassium; via carbonyl oxygenUniRule annotation
Binding sitei421 – 4211IMPUniRule annotation
Metal bindingi475 – 4751Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
Metal bindingi476 – 4761Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
Metal bindingi477 – 4771Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi301 – 3033NADUniRule annotation

GO - Molecular functioni

  1. adenyl nucleotide binding Source: InterPro
  2. IMP dehydrogenase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. GMP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

Metal-binding, NAD, Potassium

Enzyme and pathway databases

BioCyciCPAR517417:GH95-756-MONOMER.
UniPathwayiUPA00601; UER00295.

Names & Taxonomyi

Protein namesi
Recommended name:
Inosine-5'-monophosphate dehydrogenaseUniRule annotation (EC:1.1.1.205UniRule annotation)
Short name:
IMP dehydrogenaseUniRule annotation
Short name:
IMPDUniRule annotation
Short name:
IMPDHUniRule annotation
Gene namesi
Name:guaBUniRule annotation
Ordered Locus Names:Cpar_0730
OrganismiChlorobaculum parvum (strain NCIB 8327) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 263 / NCIB 8327))
Taxonomic identifieri517417 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
ProteomesiUP000008811: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494Inosine-5'-monophosphate dehydrogenasePRO_0000093694Add
BLAST

Proteomic databases

PRIDEiO50316.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi517417.Cpar_0730.

Structurei

3D structure databases

ProteinModelPortaliO50316.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini93 – 15462CBS 1UniRule annotationAdd
BLAST
Domaini158 – 21760CBS 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni341 – 3433IMP bindingUniRule annotation
Regioni364 – 3652IMP bindingUniRule annotation
Regioni388 – 3925IMP bindingUniRule annotation

Sequence similaritiesi

Belongs to the IMPDH/GMPR family.UniRule annotation
Contains 2 CBS domains.UniRule annotation

Keywords - Domaini

CBS domain, Repeat

Phylogenomic databases

eggNOGiCOG0517.
HOGENOMiHOG000165755.
KOiK00088.
OMAiYEALTFD.
OrthoDBiEOG6GTZPV.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01964. IMPDH.
InterProiIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PANTHERiPTHR11911:SF6. PTHR11911:SF6. 1 hit.
PfamiPF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFiPIRSF000130. IMPDH. 1 hit.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O50316-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDKILYDALT FDDVLLVPAY SNVLPKETVV KSRVTRNIEV NLPLVSAAMD
60 70 80 90 100
TVTEAELAIA LARAGGIGII HKNLSIDVQA RHVAKVKRFE SGIIRNPITL
110 120 130 140 150
FEDATIQEAI DLMLRHSISG IPVVERPTPE GCLLLKGIVT NRDLRMTTSS
160 170 180 190 200
NEKITTIMTT DLITAQEDID LLAAEEILMQ NKIEKLLVID EEGYLKGLIT
210 220 230 240 250
FKDIQKRKQC PDACKDMHGR LRVGAAVGIR SNTITRVDAL VEAGVDVVAV
260 270 280 290 300
DTAHGHSQAV LDMVATIKEK YPELEVIAGN VATPEAVRDL VKAGADAVKV
310 320 330 340 350
GIGPGSICTT RVVAGVGMPQ LTAIMNCAKE AAKTDTPIIA DGGIKYSGDI
360 370 380 390 400
SKALAAGADT VMMGSIFAGT DESPGETILY EGRRFKAYRG MGSLGAMSEP
410 420 430 440 450
EGSSDRYFQD ASAETKKYVP EGIEGRIPAK GPLDEVVYQL IGGLKSSMGY
460 470 480 490
CGVKNIEELK KNTRFVRITS AGLRESHPHD VMITREAPNY STSH
Length:494
Mass (Da):53,202
Last modified:October 14, 2008 - v3
Checksum:iE920D6B0B60C44AC
GO

Sequence cautioni

The sequence CAB06303.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti158 – 1581M → I in CAB06303. 1 PublicationCurated
Sequence conflicti429 – 4313AKG → QR in CAB06303. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z83933 Genomic DNA. Translation: CAB06303.1. Different initiation.
CP001099 Genomic DNA. Translation: ACF11149.1.
PIRiT17196.
RefSeqiYP_001998349.1. NC_011027.1.

Genome annotation databases

EnsemblBacteriaiACF11149; ACF11149; Cpar_0730.
GeneIDi6419655.
KEGGicpc:Cpar_0730.
PATRICi21364712. VBIChlPar72705_0729.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z83933 Genomic DNA. Translation: CAB06303.1 . Different initiation.
CP001099 Genomic DNA. Translation: ACF11149.1 .
PIRi T17196.
RefSeqi YP_001998349.1. NC_011027.1.

3D structure databases

ProteinModelPortali O50316.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 517417.Cpar_0730.

Proteomic databases

PRIDEi O50316.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACF11149 ; ACF11149 ; Cpar_0730 .
GeneIDi 6419655.
KEGGi cpc:Cpar_0730.
PATRICi 21364712. VBIChlPar72705_0729.

Phylogenomic databases

eggNOGi COG0517.
HOGENOMi HOG000165755.
KOi K00088.
OMAi YEALTFD.
OrthoDBi EOG6GTZPV.

Enzyme and pathway databases

UniPathwayi UPA00601 ; UER00295 .
BioCyci CPAR517417:GH95-756-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01964. IMPDH.
InterProi IPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view ]
PANTHERi PTHR11911:SF6. PTHR11911:SF6. 1 hit.
Pfami PF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view ]
PIRSFi PIRSF000130. IMPDH. 1 hit.
SMARTi SM00116. CBS. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR01302. IMP_dehydrog. 1 hit.
PROSITEi PS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Clustering of genes with function in the biosynthesis of bacteriochlorophyll and heme in the green sulfur bacterium Chlorobium vibrioforme."
    Petersen B.L., Moeller M.G., Stummann B.M., Henningsen K.W.
    Hereditas 125:93-96(1996)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCIB 8327.

Entry informationi

Entry nameiIMDH_CHLP8
AccessioniPrimary (citable) accession number: O50316
Secondary accession number(s): B3QMJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: October 14, 2008
Last modified: November 26, 2014
This is version 97 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3