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O50316

- IMDH_CHLP8

UniProt

O50316 - IMDH_CHLP8

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Protein
Inosine-5'-monophosphate dehydrogenase
Gene
guaB, Cpar_0730
Organism
Chlorobaculum parvum (strain NCIB 8327) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 263 / NCIB 8327))
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth By similarity.UniRule annotation

Catalytic activityi

Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

Cofactori

Potassium By similarity.UniRule annotation

Enzyme regulationi

Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei251 – 2511NAD By similarity
Metal bindingi303 – 3031Potassium; via carbonyl oxygen By similarity
Metal bindingi305 – 3051Potassium; via carbonyl oxygen By similarity
Binding sitei306 – 3061IMP By similarity
Active sitei308 – 3081Thioimidate intermediate By similarity
Metal bindingi308 – 3081Potassium; via carbonyl oxygen By similarity
Binding sitei421 – 4211IMP By similarity
Metal bindingi475 – 4751Potassium; via carbonyl oxygen; shared with tetrameric partner By similarity
Metal bindingi476 – 4761Potassium; via carbonyl oxygen; shared with tetrameric partner By similarity
Metal bindingi477 – 4771Potassium; via carbonyl oxygen; shared with tetrameric partner By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi301 – 3033NAD By similarity

GO - Molecular functioni

  1. IMP dehydrogenase activity Source: UniProtKB-HAMAP
  2. adenyl nucleotide binding Source: InterPro
  3. metal ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. GMP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

Metal-binding, NAD, Potassium

Enzyme and pathway databases

BioCyciCPAR517417:GH95-756-MONOMER.
UniPathwayiUPA00601; UER00295.

Names & Taxonomyi

Protein namesi
Recommended name:
Inosine-5'-monophosphate dehydrogenase (EC:1.1.1.205)
Short name:
IMP dehydrogenase
Short name:
IMPD
Short name:
IMPDH
Gene namesi
Name:guaB
Ordered Locus Names:Cpar_0730
OrganismiChlorobaculum parvum (strain NCIB 8327) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 263 / NCIB 8327))
Taxonomic identifieri517417 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
ProteomesiUP000008811: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494Inosine-5'-monophosphate dehydrogenaseUniRule annotation
PRO_0000093694Add
BLAST

Proteomic databases

PRIDEiO50316.

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi517417.Cpar_0730.

Structurei

3D structure databases

ProteinModelPortaliO50316.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini93 – 15462CBS 1
Add
BLAST
Domaini158 – 21760CBS 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni341 – 3433IMP binding By similarity
Regioni364 – 3652IMP binding By similarity
Regioni388 – 3925IMP binding By similarity

Sequence similaritiesi

Belongs to the IMPDH/GMPR family.
Contains 2 CBS domains.

Keywords - Domaini

CBS domain, Repeat

Phylogenomic databases

eggNOGiCOG0517.
HOGENOMiHOG000165755.
KOiK00088.
OMAiYEALTFD.
OrthoDBiEOG6GTZPV.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01964. IMPDH.
InterProiIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PANTHERiPTHR11911:SF6. PTHR11911:SF6. 1 hit.
PfamiPF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFiPIRSF000130. IMPDH. 1 hit.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O50316-1 [UniParc]FASTAAdd to Basket

« Hide

MDKILYDALT FDDVLLVPAY SNVLPKETVV KSRVTRNIEV NLPLVSAAMD    50
TVTEAELAIA LARAGGIGII HKNLSIDVQA RHVAKVKRFE SGIIRNPITL 100
FEDATIQEAI DLMLRHSISG IPVVERPTPE GCLLLKGIVT NRDLRMTTSS 150
NEKITTIMTT DLITAQEDID LLAAEEILMQ NKIEKLLVID EEGYLKGLIT 200
FKDIQKRKQC PDACKDMHGR LRVGAAVGIR SNTITRVDAL VEAGVDVVAV 250
DTAHGHSQAV LDMVATIKEK YPELEVIAGN VATPEAVRDL VKAGADAVKV 300
GIGPGSICTT RVVAGVGMPQ LTAIMNCAKE AAKTDTPIIA DGGIKYSGDI 350
SKALAAGADT VMMGSIFAGT DESPGETILY EGRRFKAYRG MGSLGAMSEP 400
EGSSDRYFQD ASAETKKYVP EGIEGRIPAK GPLDEVVYQL IGGLKSSMGY 450
CGVKNIEELK KNTRFVRITS AGLRESHPHD VMITREAPNY STSH 494
Length:494
Mass (Da):53,202
Last modified:October 14, 2008 - v3
Checksum:iE920D6B0B60C44AC
GO

Sequence cautioni

The sequence CAB06303.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti158 – 1581M → I in CAB06303. 1 Publication
Sequence conflicti429 – 4313AKG → QR in CAB06303. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z83933 Genomic DNA. Translation: CAB06303.1. Different initiation.
CP001099 Genomic DNA. Translation: ACF11149.1.
PIRiT17196.
RefSeqiYP_001998349.1. NC_011027.1.

Genome annotation databases

EnsemblBacteriaiACF11149; ACF11149; Cpar_0730.
GeneIDi6419655.
KEGGicpc:Cpar_0730.
PATRICi21364712. VBIChlPar72705_0729.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z83933 Genomic DNA. Translation: CAB06303.1 . Different initiation.
CP001099 Genomic DNA. Translation: ACF11149.1 .
PIRi T17196.
RefSeqi YP_001998349.1. NC_011027.1.

3D structure databases

ProteinModelPortali O50316.
ModBasei Search...

Protein-protein interaction databases

STRINGi 517417.Cpar_0730.

Proteomic databases

PRIDEi O50316.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACF11149 ; ACF11149 ; Cpar_0730 .
GeneIDi 6419655.
KEGGi cpc:Cpar_0730.
PATRICi 21364712. VBIChlPar72705_0729.

Phylogenomic databases

eggNOGi COG0517.
HOGENOMi HOG000165755.
KOi K00088.
OMAi YEALTFD.
OrthoDBi EOG6GTZPV.

Enzyme and pathway databases

UniPathwayi UPA00601 ; UER00295 .
BioCyci CPAR517417:GH95-756-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01964. IMPDH.
InterProi IPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view ]
PANTHERi PTHR11911:SF6. PTHR11911:SF6. 1 hit.
Pfami PF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view ]
PIRSFi PIRSF000130. IMPDH. 1 hit.
SMARTi SM00116. CBS. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR01302. IMP_dehydrog. 1 hit.
PROSITEi PS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Clustering of genes with function in the biosynthesis of bacteriochlorophyll and heme in the green sulfur bacterium Chlorobium vibrioforme."
    Petersen B.L., Moeller M.G., Stummann B.M., Henningsen K.W.
    Hereditas 125:93-96(1996)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCIB 8327.

Entry informationi

Entry nameiIMDH_CHLP8
AccessioniPrimary (citable) accession number: O50316
Secondary accession number(s): B3QMJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: October 14, 2008
Last modified: May 14, 2014
This is version 94 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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