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O50302 (CARB_GEOSE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Carbamoyl-phosphate synthase pyrimidine-specific large chain

EC=6.3.5.5
Alternative name(s):
Carbamoyl-phosphate synthetase ammonia chain
Gene names
Name:pyrAB
OrganismGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifier1422 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Protein attributes

Sequence length1064 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP-Rule MF_01210

Cofactor

Binds 4 magnesium or manganese ions per subunit By similarity.

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP-Rule MF_01210

Subunit structure

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.

Sequence similarities

Belongs to the CarB family.

Contains 2 ATP-grasp domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10641064Carbamoyl-phosphate synthase pyrimidine-specific large chain HAMAP-Rule MF_01210
PRO_0000144989

Regions

Domain133 – 327195ATP-grasp 1
Domain671 – 861191ATP-grasp 2
Nucleotide binding159 – 21658ATP By similarity
Nucleotide binding697 – 75458ATP By similarity
Region1 – 401401Carboxyphosphate synthetic domain HAMAP-Rule MF_01210
Region402 – 546145Oligomerization domain HAMAP-Rule MF_01210
Region547 – 929383Carbamoyl phosphate synthetic domain HAMAP-Rule MF_01210
Region930 – 1064135Allosteric domain HAMAP-Rule MF_01210

Sites

Metal binding2841Magnesium or manganese 1 By similarity
Metal binding2981Magnesium or manganese 1 By similarity
Metal binding2981Magnesium or manganese 2 By similarity
Metal binding3001Magnesium or manganese 2 By similarity
Metal binding8201Magnesium or manganese 3 By similarity
Metal binding8321Magnesium or manganese 3 By similarity
Metal binding8321Magnesium or manganese 4 By similarity
Metal binding8341Magnesium or manganese 4 By similarity

Sequences

Sequence LengthMass (Da)Tools
O50302 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: F5AD61003AA3FC4B

FASTA1,064116,243
        10         20         30         40         50         60 
MPKRRDIETI LVIGSGPIVI GQAAEFDYAG TQACLALKEE GYKVILVNSN PATIMTDTEI 

        70         80         90        100        110        120 
ADKVYMEPLT LDFVARIIRK ERPDAILPTL GGQTGLNLAV ELAKAGVLEE CGVEILGTKL 

       130        140        150        160        170        180 
EAIEKAEDRE QFRALMNELG EPVPESAIIH SLEEAYAFVE QIGYPVIVRP AFTLGGTGGG 

       190        200        210        220        230        240 
ICTNEEELVE IVSTGLKLSP VHQCLLERSI AGYNQIEYEV MRDANDNAIV VCNMENIDPV 

       250        260        270        280        290        300 
GIHTGDSIVV APSQTLSDRE YQLLRNASLK IIRALGIEGG CNVQLALDPD SFRYYVIEVN 

       310        320        330        340        350        360 
PRVSRSSALA SKATGYPIAK LAAKIAVGLT LDEMINPVTG KTYACFEPAL DYVVTKIPRF 

       370        380        390        400        410        420 
PFDKFESANR RLGTQMKATG EVMSIGRTFE ESLLKAVRSL EIGVHHLELN EAKTAADDVM 

       430        440        450        460        470        480 
EKRIRKAGDE RLFYIAEALR RGVTVETLHE WSQIDRFFLH KIQNIIEMET VLKNHPGDLD 

       490        500        510        520        530        540 
VLKKAKGLGF SDAAIAALWN KTERDVYALR RQEGIVPVYK MVDTCAAEFT SETPYYYSTY 

       550        560        570        580        590        600 
EEENESIVTE KPSVIVLGSG PIRIGQGIEF DYATVHCVLA IKQAGYEAII INNNPETVST 

       610        620        630        640        650        660 
DFSTSDKLYF EPLTAEDVMH VIDLEQPVGV IVQFGGQTAI NLAAELEARG VRLLGTTLED 

       670        680        690        700        710        720 
LDRAEDRDKF EQALSELGIP KPAGKTAVSV EEAVAIAEEI GYPVLVRPSY VLGGRAMEIV 

       730        740        750        760        770        780 
YNREELLHYM EHAVRVNPQH PVLVDRYITG KEVEVDAIAD GETVVIPGIM EHIERAGVHS 

       790        800        810        820        830        840 
GDSIAVYPPQ TLSDDVIAKI TDYTVKLARG LHIVGLLNIQ FVVAGSDVYV LEVNPRSSRT 

       850        860        870        880        890        900 
VPFLSKITGV PMANLATKAI LGAKLADMGY ETGVCPVRPG VYVKVPVFSF AKLRNVDISL 

       910        920        930        940        950        960 
GPEMKSTGEV IGKDVTFEKA LYKGLVASGI QIQPHGAVLL TVADKDKEEA VELARRFADI 

       970        980        990       1000       1010       1020 
GYQLLATNGT AETLKAAGIP VTVVNKIHSA SPNILDVIRQ GKAQVVINTL TKGKQPESDG 

      1030       1040       1050       1060 
FRIRREVENG IPCLTSLDTA RAMLQVLESM TFSTTAMTEG LVRS 

« Hide

References

[1]"The pyrAb gene coding for the large subunit of carbamoylphosphate synthetase from Bacillus stearothermophilus: molecular cloning and functional characterization."
Vlaskova H., Krasny L., Fucik V., Jonak J.
Folia Biol. (Praha) 44:163-172(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: CCM 2184.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ001805 Genomic DNA. Translation: CAA05020.1.
PIRT44419.

3D structure databases

ProteinModelPortalO50302.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00070; UER00115.

Family and domain databases

Gene3D1.10.1030.10. 1 hit.
3.30.1490.20. 2 hits.
3.30.470.20. 2 hits.
3.40.50.1380. 1 hit.
3.40.50.20. 2 hits.
HAMAPMF_01210_A. CPSase_L_chain_A.
MF_01210_B. CPSase_L_chain_B.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006275. CarbamoylP_synth_lsu.
IPR005481. CarbamoylP_synth_lsu_N.
IPR005480. CarbamoylP_synth_lsu_oligo.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR005483. CbamoylP_synth_lsu_CPSase_dom.
IPR011607. MGS-like_dom.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamPF00289. CPSase_L_chain. 2 hits.
PF02786. CPSase_L_D2. 2 hits.
PF02787. CPSase_L_D3. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PRINTSPR00098. CPSASE.
SMARTSM01096. CPSase_L_D3. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMSSF48108. SSF48108. 1 hit.
SSF52335. SSF52335. 1 hit.
SSF52440. SSF52440. 2 hits.
TIGRFAMsTIGR01369. CPSaseII_lrg. 1 hit.
PROSITEPS50975. ATP_GRASP. 2 hits.
PS00866. CPSASE_1. 2 hits.
PS00867. CPSASE_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCARB_GEOSE
AccessionPrimary (citable) accession number: O50302
Entry history
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: June 1, 1998
Last modified: February 19, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways