Carbamoyl-phosphate synthase pyrimidine-specific large chain

Preferred order of sections

Names and origin

Protein names

Recommended name:
Carbamoyl-phosphate synthase pyrimidine-specific large chain
EC=6.3.5.5

Alternative name(s):
Carbamoyl-phosphate synthetase ammonia chain

Gene names

Name:

pyrAB

Organism

Geobacillus stearothermophilus (Bacillus stearothermophilus)

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	1064 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO₃^- + H₂O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP-Rule MF_01210
Cofactor	Binds 4 magnesium or manganese ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP-Rule MF_01210
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.
Sequence similarities	Belongs to the CarB family. Contains 2 ATP-grasp domains.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Domain	Repeat
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological_process	'de novo' UMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1064

1064

Carbamoyl-phosphate synthase pyrimidine-specific large chain HAMAP-Rule MF_01210

PRO_0000144989

Regions

Domain

133 – 327

195

ATP-grasp 1

Domain

671 – 861

191

ATP-grasp 2

Nucleotide binding

159 – 216

58

ATP By similarity

Nucleotide binding

697 – 754

58

ATP By similarity

Region

1 – 401

401

Carboxyphosphate synthetic domain HAMAP-Rule MF_01210

Region

402 – 546

145

Oligomerization domain HAMAP-Rule MF_01210

Region

547 – 929

383

Carbamoyl phosphate synthetic domain HAMAP-Rule MF_01210

Region

930 – 1064

135

Allosteric domain HAMAP-Rule MF_01210

Sites

Metal binding

284

1

Magnesium or manganese 1 By similarity

Metal binding

298

1

Magnesium or manganese 1 By similarity

Metal binding

298

1

Magnesium or manganese 2 By similarity

Metal binding

300

1

Magnesium or manganese 2 By similarity

Metal binding

820

1

Magnesium or manganese 3 By similarity

Metal binding

832

1

Magnesium or manganese 3 By similarity

Metal binding

832

1

Magnesium or manganese 4 By similarity

Metal binding

834

1

Magnesium or manganese 4 By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

O50302 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: F5AD61003AA3FC4B
Show »

FASTA

1,064

116,243

        10         20         30         40         50         60 
MPKRRDIETI LVIGSGPIVI GQAAEFDYAG TQACLALKEE GYKVILVNSN PATIMTDTEI 

        70         80         90        100        110        120 
ADKVYMEPLT LDFVARIIRK ERPDAILPTL GGQTGLNLAV ELAKAGVLEE CGVEILGTKL 

       130        140        150        160        170        180 
EAIEKAEDRE QFRALMNELG EPVPESAIIH SLEEAYAFVE QIGYPVIVRP AFTLGGTGGG 

       190        200        210        220        230        240 
ICTNEEELVE IVSTGLKLSP VHQCLLERSI AGYNQIEYEV MRDANDNAIV VCNMENIDPV 

       250        260        270        280        290        300 
GIHTGDSIVV APSQTLSDRE YQLLRNASLK IIRALGIEGG CNVQLALDPD SFRYYVIEVN 

       310        320        330        340        350        360 
PRVSRSSALA SKATGYPIAK LAAKIAVGLT LDEMINPVTG KTYACFEPAL DYVVTKIPRF 

       370        380        390        400        410        420 
PFDKFESANR RLGTQMKATG EVMSIGRTFE ESLLKAVRSL EIGVHHLELN EAKTAADDVM 

       430        440        450        460        470        480 
EKRIRKAGDE RLFYIAEALR RGVTVETLHE WSQIDRFFLH KIQNIIEMET VLKNHPGDLD 

       490        500        510        520        530        540 
VLKKAKGLGF SDAAIAALWN KTERDVYALR RQEGIVPVYK MVDTCAAEFT SETPYYYSTY 

       550        560        570        580        590        600 
EEENESIVTE KPSVIVLGSG PIRIGQGIEF DYATVHCVLA IKQAGYEAII INNNPETVST 

       610        620        630        640        650        660 
DFSTSDKLYF EPLTAEDVMH VIDLEQPVGV IVQFGGQTAI NLAAELEARG VRLLGTTLED 

       670        680        690        700        710        720 
LDRAEDRDKF EQALSELGIP KPAGKTAVSV EEAVAIAEEI GYPVLVRPSY VLGGRAMEIV 

       730        740        750        760        770        780 
YNREELLHYM EHAVRVNPQH PVLVDRYITG KEVEVDAIAD GETVVIPGIM EHIERAGVHS 

       790        800        810        820        830        840 
GDSIAVYPPQ TLSDDVIAKI TDYTVKLARG LHIVGLLNIQ FVVAGSDVYV LEVNPRSSRT 

       850        860        870        880        890        900 
VPFLSKITGV PMANLATKAI LGAKLADMGY ETGVCPVRPG VYVKVPVFSF AKLRNVDISL 

       910        920        930        940        950        960 
GPEMKSTGEV IGKDVTFEKA LYKGLVASGI QIQPHGAVLL TVADKDKEEA VELARRFADI 

       970        980        990       1000       1010       1020 
GYQLLATNGT AETLKAAGIP VTVVNKIHSA SPNILDVIRQ GKAQVVINTL TKGKQPESDG 

      1030       1040       1050       1060 
FRIRREVENG IPCLTSLDTA RAMLQVLESM TFSTTAMTEG LVRS

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References

[1]

"The pyrAb gene coding for the large subunit of carbamoylphosphate synthetase from Bacillus stearothermophilus: molecular cloning and functional characterization."
Vlaskova H., Krasny L., Fucik V., Jonak J.
Folia Biol. (Praha) 44:163-172(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: CCM 2184.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ001805 Genomic DNA. Translation: CAA05020.1.
PIR	T44419.
3D structure databases
ProteinModelPortal	O50302.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00070; UER00115.
Family and domain databases
Gene3D	1.10.1030.10. 1 hit. 3.30.1490.20. 2 hits. 3.30.470.20. 2 hits. 3.40.50.1380. 1 hit. 3.40.50.20. 2 hits.
HAMAP	MF_01210_B. CPSase_L_chain_B.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR006275. CarbamoylP_synth_lsu. IPR005481. CarbamoylP_synth_lsu_N. IPR005480. CarbamoylP_synth_lsu_oligo. IPR005479. CbamoylP_synth_lsu-like_ATP-bd. IPR005483. CbamoylP_synth_lsu_CPSase_dom. IPR011607. MGS-like_dom. IPR016185. PreATP-grasp_dom. [Graphical view]
Pfam	PF00289. CPSase_L_chain. 2 hits. PF02786. CPSase_L_D2. 2 hits. PF02787. CPSase_L_D3. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PRINTS	PR00098. CPSASE.
SMART	SM01096. CPSase_L_D3. 1 hit. SM00851. MGS. 1 hit. [Graphical view]
SUPFAM	SSF48108. CarbamoylP_synth_lsu_oligo. 1 hit. SSF52335. MGS-like_dom. 1 hit. SSF52440. PreATP-grasp-like. 2 hits.
TIGRFAMs	TIGR01369. CPSaseII_lrg. 1 hit.
PROSITE	PS50975. ATP_GRASP. 2 hits. PS00866. CPSASE_1. 2 hits. PS00867. CPSASE_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARB_GEOSE

Accession

Primary (citable) accession number: O50302

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2002
Last sequence update:	June 1, 1998
Last modified:	April 3, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents