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O50302

- CARB_GEOSE

UniProt

O50302 - CARB_GEOSE

Protein

Carbamoyl-phosphate synthase pyrimidine-specific large chain

Gene

pyrAB

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.UniRule annotation

    Cofactori

    Binds 4 magnesium or manganese ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi284 – 2841Magnesium or manganese 1UniRule annotation
    Metal bindingi298 – 2981Magnesium or manganese 1UniRule annotation
    Metal bindingi298 – 2981Magnesium or manganese 2UniRule annotation
    Metal bindingi300 – 3001Magnesium or manganese 2UniRule annotation
    Metal bindingi820 – 8201Magnesium or manganese 3UniRule annotation
    Metal bindingi832 – 8321Magnesium or manganese 3UniRule annotation
    Metal bindingi832 – 8321Magnesium or manganese 4UniRule annotation
    Metal bindingi834 – 8341Magnesium or manganese 4UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi159 – 21658ATPUniRule annotationAdd
    BLAST
    Nucleotide bindingi697 – 75458ATPUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Pyrimidine biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00070; UER00115.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carbamoyl-phosphate synthase pyrimidine-specific large chain (EC:6.3.5.5UniRule annotation)
    Alternative name(s):
    Carbamoyl-phosphate synthetase ammonia chainUniRule annotation
    Gene namesi
    Name:pyrAB
    OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
    Taxonomic identifieri1422 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10641064Carbamoyl-phosphate synthase pyrimidine-specific large chainPRO_0000144989Add
    BLAST

    Interactioni

    Subunit structurei

    Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.

    Structurei

    3D structure databases

    ProteinModelPortaliO50302.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini133 – 327195ATP-grasp 1UniRule annotationAdd
    BLAST
    Domaini671 – 861191ATP-grasp 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 401401Carboxyphosphate synthetic domainAdd
    BLAST
    Regioni402 – 546145Oligomerization domainAdd
    BLAST
    Regioni547 – 929383Carbamoyl phosphate synthetic domainAdd
    BLAST
    Regioni930 – 1064135Allosteric domainAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CarB family.UniRule annotation
    Contains 2 ATP-grasp domains.UniRule annotation

    Keywords - Domaini

    Repeat

    Family and domain databases

    Gene3Di1.10.1030.10. 1 hit.
    3.30.1490.20. 2 hits.
    3.30.470.20. 2 hits.
    3.40.50.1380. 1 hit.
    3.40.50.20. 2 hits.
    HAMAPiMF_01210_A. CPSase_L_chain_A.
    MF_01210_B. CPSase_L_chain_B.
    InterProiIPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR006275. CarbamoylP_synth_lsu.
    IPR005481. CarbamoylP_synth_lsu_N.
    IPR005480. CarbamoylP_synth_lsu_oligo.
    IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
    IPR005483. CbamoylP_synth_lsu_CPSase_dom.
    IPR011607. MGS-like_dom.
    IPR016185. PreATP-grasp_dom.
    [Graphical view]
    PfamiPF00289. CPSase_L_chain. 2 hits.
    PF02786. CPSase_L_D2. 2 hits.
    PF02787. CPSase_L_D3. 1 hit.
    PF02142. MGS. 1 hit.
    [Graphical view]
    PRINTSiPR00098. CPSASE.
    SMARTiSM01096. CPSase_L_D3. 1 hit.
    SM00851. MGS. 1 hit.
    [Graphical view]
    SUPFAMiSSF48108. SSF48108. 1 hit.
    SSF52335. SSF52335. 1 hit.
    SSF52440. SSF52440. 2 hits.
    TIGRFAMsiTIGR01369. CPSaseII_lrg. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 2 hits.
    PS00866. CPSASE_1. 2 hits.
    PS00867. CPSASE_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O50302-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPKRRDIETI LVIGSGPIVI GQAAEFDYAG TQACLALKEE GYKVILVNSN     50
    PATIMTDTEI ADKVYMEPLT LDFVARIIRK ERPDAILPTL GGQTGLNLAV 100
    ELAKAGVLEE CGVEILGTKL EAIEKAEDRE QFRALMNELG EPVPESAIIH 150
    SLEEAYAFVE QIGYPVIVRP AFTLGGTGGG ICTNEEELVE IVSTGLKLSP 200
    VHQCLLERSI AGYNQIEYEV MRDANDNAIV VCNMENIDPV GIHTGDSIVV 250
    APSQTLSDRE YQLLRNASLK IIRALGIEGG CNVQLALDPD SFRYYVIEVN 300
    PRVSRSSALA SKATGYPIAK LAAKIAVGLT LDEMINPVTG KTYACFEPAL 350
    DYVVTKIPRF PFDKFESANR RLGTQMKATG EVMSIGRTFE ESLLKAVRSL 400
    EIGVHHLELN EAKTAADDVM EKRIRKAGDE RLFYIAEALR RGVTVETLHE 450
    WSQIDRFFLH KIQNIIEMET VLKNHPGDLD VLKKAKGLGF SDAAIAALWN 500
    KTERDVYALR RQEGIVPVYK MVDTCAAEFT SETPYYYSTY EEENESIVTE 550
    KPSVIVLGSG PIRIGQGIEF DYATVHCVLA IKQAGYEAII INNNPETVST 600
    DFSTSDKLYF EPLTAEDVMH VIDLEQPVGV IVQFGGQTAI NLAAELEARG 650
    VRLLGTTLED LDRAEDRDKF EQALSELGIP KPAGKTAVSV EEAVAIAEEI 700
    GYPVLVRPSY VLGGRAMEIV YNREELLHYM EHAVRVNPQH PVLVDRYITG 750
    KEVEVDAIAD GETVVIPGIM EHIERAGVHS GDSIAVYPPQ TLSDDVIAKI 800
    TDYTVKLARG LHIVGLLNIQ FVVAGSDVYV LEVNPRSSRT VPFLSKITGV 850
    PMANLATKAI LGAKLADMGY ETGVCPVRPG VYVKVPVFSF AKLRNVDISL 900
    GPEMKSTGEV IGKDVTFEKA LYKGLVASGI QIQPHGAVLL TVADKDKEEA 950
    VELARRFADI GYQLLATNGT AETLKAAGIP VTVVNKIHSA SPNILDVIRQ 1000
    GKAQVVINTL TKGKQPESDG FRIRREVENG IPCLTSLDTA RAMLQVLESM 1050
    TFSTTAMTEG LVRS 1064
    Length:1,064
    Mass (Da):116,243
    Last modified:June 1, 1998 - v1
    Checksum:iF5AD61003AA3FC4B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ001805 Genomic DNA. Translation: CAA05020.1.
    PIRiT44419.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ001805 Genomic DNA. Translation: CAA05020.1 .
    PIRi T44419.

    3D structure databases

    ProteinModelPortali O50302.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00070 ; UER00115 .

    Family and domain databases

    Gene3Di 1.10.1030.10. 1 hit.
    3.30.1490.20. 2 hits.
    3.30.470.20. 2 hits.
    3.40.50.1380. 1 hit.
    3.40.50.20. 2 hits.
    HAMAPi MF_01210_A. CPSase_L_chain_A.
    MF_01210_B. CPSase_L_chain_B.
    InterProi IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR006275. CarbamoylP_synth_lsu.
    IPR005481. CarbamoylP_synth_lsu_N.
    IPR005480. CarbamoylP_synth_lsu_oligo.
    IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
    IPR005483. CbamoylP_synth_lsu_CPSase_dom.
    IPR011607. MGS-like_dom.
    IPR016185. PreATP-grasp_dom.
    [Graphical view ]
    Pfami PF00289. CPSase_L_chain. 2 hits.
    PF02786. CPSase_L_D2. 2 hits.
    PF02787. CPSase_L_D3. 1 hit.
    PF02142. MGS. 1 hit.
    [Graphical view ]
    PRINTSi PR00098. CPSASE.
    SMARTi SM01096. CPSase_L_D3. 1 hit.
    SM00851. MGS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48108. SSF48108. 1 hit.
    SSF52335. SSF52335. 1 hit.
    SSF52440. SSF52440. 2 hits.
    TIGRFAMsi TIGR01369. CPSaseII_lrg. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 2 hits.
    PS00866. CPSASE_1. 2 hits.
    PS00867. CPSASE_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The pyrAb gene coding for the large subunit of carbamoylphosphate synthetase from Bacillus stearothermophilus: molecular cloning and functional characterization."
      Vlaskova H., Krasny L., Fucik V., Jonak J.
      Folia Biol. (Praha) 44:163-172(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: CCM 2184.

    Entry informationi

    Entry nameiCARB_GEOSE
    AccessioniPrimary (citable) accession number: O50302
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 2, 2002
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3