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Protein

Bifunctional enzyme CysN/CysC

Gene

cysNC

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

ATP sulfurylase may be the GTPase, regulating ATP sulfurylase activity.By similarity
APS kinase catalyzes the synthesis of activated sulfate.By similarity

Catalytic activityi

ATP + sulfate = diphosphate + adenylyl sulfate.
ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate.

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 1 and 2 of the subpathway that synthesizes sulfite from sulfate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase subunit 2 (cysD), Bifunctional enzyme CysN/CysC (cysNC)
  2. Bifunctional enzyme CysN/CysC (cysNC), Adenylyl-sulfate kinase (cysC)
  3. Phosphoadenosine phosphosulfate reductase (cysH)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi31 – 388GTPBy similarity
Nucleotide bindingi110 – 1145GTPBy similarity
Nucleotide bindingi165 – 1684GTPBy similarity
Nucleotide bindingi472 – 4798ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Keywords - Molecular functioni

Kinase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

ATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00140; UER00204.
UPA00140; UER00205.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional enzyme CysN/CysC
Including the following 2 domains:
Sulfate adenylyltransferase subunit 1 (EC:2.7.7.4)
Alternative name(s):
ATP-sulfurylase large subunit
Sulfate adenylate transferase
Short name:
SAT
Adenylyl-sulfate kinase (EC:2.7.1.25)
Alternative name(s):
APS kinase
ATP adenosine-5'-phosphosulfate 3'-phosphotransferase
Gene namesi
Name:cysNC
Synonyms:cysN
Ordered Locus Names:PA4442
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA4442.

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 633633Bifunctional enzyme CysN/CysCPRO_0000091538Add
BLAST

Proteomic databases

PaxDbiO50274.

Interactioni

Subunit structurei

Heterodimer composed of CysD, the smaller subunit, and CysNC.By similarity

Protein-protein interaction databases

STRINGi208964.PA4442.

Structurei

3D structure databases

ProteinModelPortaliO50274.
SMRiO50274. Positions 16-465, 467-627.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 241220tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 463463Sulfate adenylyltransferaseAdd
BLAST
Regioni31 – 388G1By similarity
Regioni89 – 935G2By similarity
Regioni110 – 1134G3By similarity
Regioni165 – 1684G4By similarity
Regioni204 – 2063G5By similarity
Regioni464 – 633170Adenylyl-sulfate kinaseAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the APS kinase family.Curated

Phylogenomic databases

eggNOGiENOG4105C3T. Bacteria.
COG0529. LUCA.
COG2895. LUCA.
HOGENOMiHOG000229289.
InParanoidiO50274.
KOiK00955.
OMAiIQDVYKF.
OrthoDBiEOG6GR38Q.
PhylomeDBiO50274.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_00062. Sulf_adenylyltr_sub1.
MF_00065. Adenylyl_sulf_kinase.
InterProiIPR002891. APS_kinase.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR011779. SO4_adenylTrfase_lsu.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00455. apsK. 1 hit.
TIGR02034. CysN. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O50274-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHQSDLISE DILAYLGQHE RKELLRFLTC GNVDDGKSTL IGRLLHDSKM
60 70 80 90 100
IYEDHLEAIT RDSKKVGTTG DDVDLALLVD GLQAEREQGI TIDVAYRYFS
110 120 130 140 150
TAKRKFIIAD TPGHEQYTRN MATGASTCDL AIILIDARYG VQTQTRRHSF
160 170 180 190 200
IASLLGIRHI VVAINKMDLK DFDQGVFEQI KADYLAFAEK IGLKTSSLHF
210 220 230 240 250
VPMSALKGDN VVNKSERSPW YAGQSLMEIL ETVEIAADRN LDDMRFPVQY
260 270 280 290 300
VNRPNLNFRG FAGTLASGVV RKGDEVVALP SGKGSKVKSI VTFEGELEQA
310 320 330 340 350
GPGQAVTLTL EDEIDVSRGD MLVHADNRPL VTDGFDAMLV WMAEEPMLPG
360 370 380 390 400
KKYDIKRATS YVPGSIPSIV HKVDVNTLER TPGSELKLNE IARVKVSLDA
410 420 430 440 450
PIALDGYEQN RTTGAFIVID RLTNGTVGAG MIVSAPPAAH GSSAHHGSNA
460 470 480 490 500
HVTREERAGR FGQQPATVLF SGLSGAGKST LAYAVERKLF DMGRAVYVLD
510 520 530 540 550
GQNLRHDLNK GLPQDRAGRT ENWLRTAHVA KQFNEAGLIS LCAFVAPSAE
560 570 580 590 600
GREQARALIG AERLITVYVQ ASPQVCRERD PQGLYAAGED NIPGESFPYD
610 620 630
VPLDADLVID TQALSVEDGV KQVLDLLRER QAI
Length:633
Mass (Da):69,269
Last modified:June 1, 1998 - v1
Checksum:i4D56B6948F2A4E83
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF035608 Genomic DNA. Translation: AAC46387.1.
AE004091 Genomic DNA. Translation: AAG07830.1.
PIRiD83091.
RefSeqiNP_253132.1. NC_002516.2.
WP_003110029.1. NZ_ASJY01000727.1.

Genome annotation databases

EnsemblBacteriaiAAG07830; AAG07830; PA4442.
GeneIDi880979.
KEGGipae:PA4442.
PATRICi19843609. VBIPseAer58763_4651.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF035608 Genomic DNA. Translation: AAC46387.1.
AE004091 Genomic DNA. Translation: AAG07830.1.
PIRiD83091.
RefSeqiNP_253132.1. NC_002516.2.
WP_003110029.1. NZ_ASJY01000727.1.

3D structure databases

ProteinModelPortaliO50274.
SMRiO50274. Positions 16-465, 467-627.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA4442.

Proteomic databases

PaxDbiO50274.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG07830; AAG07830; PA4442.
GeneIDi880979.
KEGGipae:PA4442.
PATRICi19843609. VBIPseAer58763_4651.

Organism-specific databases

PseudoCAPiPA4442.

Phylogenomic databases

eggNOGiENOG4105C3T. Bacteria.
COG0529. LUCA.
COG2895. LUCA.
HOGENOMiHOG000229289.
InParanoidiO50274.
KOiK00955.
OMAiIQDVYKF.
OrthoDBiEOG6GR38Q.
PhylomeDBiO50274.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00204.
UPA00140; UER00205.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_00062. Sulf_adenylyltr_sub1.
MF_00065. Adenylyl_sulf_kinase.
InterProiIPR002891. APS_kinase.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR011779. SO4_adenylTrfase_lsu.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00455. apsK. 1 hit.
TIGR02034. CysN. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Regulation of the sulfate starvation response in Pseudomonas aeruginosa: role of cysteine biosynthetic intermediates."
    Hummerjohann J., Kuttel E., Quadroni M., Ragaller J., Leisinger T., Kertesz M.A.
    Microbiology 144:1375-1386(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiCYSNC_PSEAE
AccessioniPrimary (citable) accession number: O50274
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: May 11, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

It is not obvious if the APS kinase domain is functional; the conserved active site serine (position 546) is replaced by an alanine. Furthermore P.aeruginosa seems to harbor a bona fide single domain APS kinase (CysC).Curated

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.