ID   ASTE_PSEAE              Reviewed;         332 AA.
AC   O50177;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   16-JUN-2009, entry version 55.
DE   RecName: Full=Succinylglutamate desuccinylase;
DE            EC=3.5.1.96;
GN   Name=astE; Synonyms=aruE; OrderedLocusNames=PA0901;
OS   Pseudomonas aeruginosa.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
RX   MEDLINE=98053840; PubMed=9393691;
RA   Itoh Y.;
RT   "Cloning and characterization of the aru genes encoding enzymes of the
RT   catabolic arginine succinyltransferase pathway in Pseudomonas
RT   aeruginosa.";
RL   J. Bacteriol. 179:7280-7290(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
RX   MEDLINE=20437337; PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T.,
RA   Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an
RT   opportunistic pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Transforms N(2)-succinylglutamate into succinate and
CC       glutamate.
CC   -!- CATALYTIC ACTIVITY: N-succinyl-L-glutamate + H(2)O = succinate +
CC       L-glutamate.
CC   -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC   -!- PATHWAY: Amino-acid degradation; L-arginine degradation via AST
CC       pathway; L-glutamate and succinate from L-arginine: step 5/5.
CC   -!- SIMILARITY: Belongs to the aspA/astE family. Succinylglutamate
CC       desuccinylase subfamily.
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DR   EMBL; AF011922; AAC46014.1; -; Genomic_DNA.
DR   EMBL; AE004091; AAG04290.1; -; Genomic_DNA.
DR   PIR; F83533; F83533.
DR   RefSeq; NP_249592.1; -.
DR   GeneID; 878165; -.
DR   GenomeReviews; AE004091_GR; PA0901.
DR   KEGG; pae:PA0901; -.
DR   PseudoCAP; PA0901; -.
DR   HOGENOM; O50177; -.
DR   OMA; O50177; EKFAIYP.
DR   BioCyc; PAER208964:PA0901-MON; -.
DR   BRENDA; 3.5.1.96; 354.
DR   GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:HAMAP.
DR   GO; GO:0009017; F:succinylglutamate desuccinylase activity; IEA:EC.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0019544; P:arginine catabolic process to glutamate; IEA:HAMAP.
DR   HAMAP; MF_00767; -; 1.
DR   InterPro; IPR007036; Aste_AspA.
DR   InterPro; IPR016681; SuccinylGlu_desuccinylase.
DR   Pfam; PF04952; AstE_AspA; 1.
DR   PIRSF; PIRSF017020; AstE; 1.
DR   TIGRFAMs; TIGR03242; arg_catab_astE; 1.
PE   3: Inferred from homology;
KW   Arginine metabolism; Complete proteome; Hydrolase; Metal-binding;
KW   Zinc.
FT   CHAIN         1    332       Succinylglutamate desuccinylase.
FT                                /FTId=PRO_0000174643.
FT   ACT_SITE    215    215       Potential.
FT   METAL        59     59       Zinc (By similarity).
FT   METAL        62     62       Zinc (By similarity).
FT   METAL       151    151       Zinc (By similarity).
SQ   SEQUENCE   332 AA;  36909 MW;  EE8F131836747F59 CRC64;
     MLALGKLLDL TLAGREPTEK IQLTADGTRL HWLAEGALEV TPIGARDNGV DLLLSAGIHG
     NETAPIELLE RLIRKVAAGT LKPAARVLFL FGNPEAIRRG ERYVEQDMNR LFNGRHEEGS
     GNEAFRAAEL ERLAQVFFSK TERVHLHYDL HTAIRGSKIE QFALYPWAEG RQHSRSELAR
     LRDAGIEAVL LQNKPGITFS AYTYGQLGAE AFTLELGKAR PFGENQEVNL ERLERSLELL
     IDGSEEQPDG SRLDGLKLFS VSREVIKHSD HFRLHLDDDV ANFTELSPGY LLAEDIGGTR
     WVVDEVGARI IFPNPRVKNG LRAGILVVPA KL
//