Reviewed, UniProtKB/Swiss-Prot O50177 (ASTE_PSEAE)

Names and origin

Protein names

Recommended name:
    Succinylglutamate desuccinylase
    EC=3.5.1.96

Gene names

Name:	astE
Synonyms:	aruE
Ordered Locus Names:	PA0901

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Transforms N(2)-succinylglutamate into succinate and glutamate. HAMAP MF_00767
Catalytic activity	N-succinyl-L-glutamate + H2O = succinate + L-glutamate. HAMAP MF_00767
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 5/5. HAMAP MF_00767
Sequence similarities	Belongs to the aspA/astE family. Succinylglutamate desuccinylase subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrolase activity, acting on ester bonds Inferred from electronic annotation. Source: HAMAP succinylglutamate desuccinylase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	Succinylglutamate desuccinylase HAMAP MF_00767		PRO_0000174643
Sites
	Active site	215	1	Potential
	Metal binding	59	1	Zinc By similarity
	Metal binding	62	1	Zinc By similarity
	Metal binding	151	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	O50177-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: EE8F131836747F59	FASTA	332	36,909
	10 20 30 40 50 60 MLALGKLLDL TLAGREPTEK IQLTADGTRL HWLAEGALEV TPIGARDNGV DLLLSAGIHG 70 80 90 100 110 120 NETAPIELLE RLIRKVAAGT LKPAARVLFL FGNPEAIRRG ERYVEQDMNR LFNGRHEEGS 130 140 150 160 170 180 GNEAFRAAEL ERLAQVFFSK TERVHLHYDL HTAIRGSKIE QFALYPWAEG RQHSRSELAR 190 200 210 220 230 240 LRDAGIEAVL LQNKPGITFS AYTYGQLGAE AFTLELGKAR PFGENQEVNL ERLERSLELL 250 260 270 280 290 300 IDGSEEQPDG SRLDGLKLFS VSREVIKHSD HFRLHLDDDV ANFTELSPGY LLAEDIGGTR 310 320 330 WVVDEVGARI IFPNPRVKNG LRAGILVVPA KL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the aru genes encoding enzymes of the catabolic arginine succinyltransferase pathway in Pseudomonas aeruginosa." Itoh Y. J. Bacteriol. 179:7280-7290(1997) [PubMed: 9393691] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF011922 Genomic DNA. Translation: AAC46014.1. AE004091 Genomic DNA. Translation: AAG04290.1.
PIR	F83533.
RefSeq	NP_249592.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	878165.
GenomeReviews	Gene locus PA0901 in contig AE004091_GR.
KEGG	pae:PA0901.
Organism-specific databases
PseudoCAP	PA0901.
CMR	Search...
Phylogenomic databases
HOGENOM	O50177.
OMA	O50177. EKFAIYP.
Enzyme and pathway databases
BioCyc	PAER208964:PA0901-MON.
BRENDA	3.5.1.96. 354.
Family and domain databases
HAMAP	MF_00767. [Tree]
InterPro	IPR007036. Aste_AspA. IPR016681. SuccinylGlu_desuccinylase. [Graphical view]
Pfam	PF04952. AstE_AspA. 1 hit. [Graphical view]
PIRSF	PIRSF017020. AstE. 1 hit.
TIGRFAMs	TIGR03242. arg_catab_astE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASTE_PSEAE

Accession

Primary (citable) accession number: O50177

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)