ID   ASTB_PSEAE              Reviewed;         448 AA.
AC   O50175;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   16-JUN-2009, entry version 52.
DE   RecName: Full=N-succinylarginine dihydrolase;
DE            EC=3.5.3.23;
GN   Name=astB; Synonyms=aruB; OrderedLocusNames=PA0899;
OS   Pseudomonas aeruginosa.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
RX   MEDLINE=98053840; PubMed=9393691;
RA   Itoh Y.;
RT   "Cloning and characterization of the aru genes encoding enzymes of the
RT   catabolic arginine succinyltransferase pathway in Pseudomonas
RT   aeruginosa.";
RL   J. Bacteriol. 179:7280-7290(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
RX   MEDLINE=20437337; PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T.,
RA   Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an
RT   opportunistic pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Catalyzes the hydrolysis of N(2)-succinylarginine into
CC       N(2)-succinylornithine, ammonia and CO(2).
CC   -!- CATALYTIC ACTIVITY: N(2)-succinyl-L-arginine + 2 H(2)O = N(2)-
CC       succinyl-L-ornithine + 2 NH(3) + CO(2).
CC   -!- PATHWAY: Amino-acid degradation; L-arginine degradation via AST
CC       pathway; L-glutamate and succinate from L-arginine: step 2/5.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SIMILARITY: Belongs to the succinylarginine dihydrolase family.
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DR   EMBL; AF011922; AAC46013.1; -; Genomic_DNA.
DR   EMBL; AE004091; AAG04288.1; -; Genomic_DNA.
DR   PIR; D83533; D83533.
DR   RefSeq; NP_249590.1; -.
DR   GeneID; 878588; -.
DR   GenomeReviews; AE004091_GR; PA0899.
DR   KEGG; pae:PA0899; -.
DR   PseudoCAP; PA0899; -.
DR   HOGENOM; O50175; -.
DR   OMA; O50175; KMKALME.
DR   BioCyc; PAER208964:PA0899-MON; -.
DR   BRENDA; 3.5.3.23; 354.
DR   GO; GO:0009015; F:N-succinylarginine dihydrolase activity; IEA:HAMAP.
DR   GO; GO:0019544; P:arginine catabolic process to glutamate; IEA:HAMAP.
DR   HAMAP; MF_01172; -; 1.
DR   InterPro; IPR007079; SuccinylArg_d-Hdrlase_AstB.
DR   Gene3D; G3DSA:3.75.10.20; SuccinylArg_di_hydro; 1.
DR   Pfam; PF04996; AstB; 1.
DR   TIGRFAMs; TIGR03241; arg_catab_astB; 1.
PE   3: Inferred from homology;
KW   Arginine metabolism; Complete proteome; Hydrolase.
FT   CHAIN         1    448       N-succinylarginine dihydrolase.
FT                                /FTId=PRO_0000064716.
FT   REGION       19     28       Substrate binding (By similarity).
FT   REGION      137    138       Substrate binding (By similarity).
FT   ACT_SITE    174    174       By similarity.
FT   ACT_SITE    250    250       By similarity.
FT   ACT_SITE    371    371       Nucleophile (By similarity).
FT   BINDING     110    110       Substrate (By similarity).
FT   BINDING     214    214       Substrate (By similarity).
FT   BINDING     252    252       Substrate (By similarity).
FT   BINDING     365    365       Substrate (By similarity).
SQ   SEQUENCE   448 AA;  48875 MW;  39F9F379CCC16196 CRC64;
     MNAHEVNFDG LVGPTHNYGG LSYGNVASQS NSQAVSNPKE AAKQGLAKMK ALMEMGFKQG
     VLAPQARPDT AALRSLGFSG SDEEVIRRAA KEAMPLLAAC SSASSMWTAN AATVSPSADT
     ADGRVHFTAA NLNCKFHRSI EHPTTSRVLA AMFNDERHFA HHAALPAVSQ FGDEGAANHT
     RFCKDYGDAG VEFFVFGRSA FDSRFPAPQR YPARQTLEAC QAVARLHGLS EAGVVYAQQN
     PAVIDQGVFH NDVISVGNGE VLFHHEDAFL DTEKVLAELH DKLGRRGGRF RAICVPRDQV
     AVEDAVKSYL FNSQLLSKAD GSMLLVVPEE CRNNPRVWNY LDQLTGDDGP IREVKVFDLK
     QSMQNGGGPA CLRLRVALQE RELAAVNPGV IMSAGLYDTL VAWVDRHYRD RLSETDLADP
     QLLLECRTAL DELTQILKLG SVYSFQLD
//