Reviewed, UniProtKB/Swiss-Prot O50175 (ASTB_PSEAE)

Names and origin

Protein names

Recommended name:
    N-succinylarginine dihydrolase
    EC=3.5.3.23

Gene names

Name:	astB
Synonyms:	aruB
Ordered Locus Names:	PA0899

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the hydrolysis of N(2)-succinylarginine into N(2)-succinylornithine, ammonia and CO2. HAMAP MF_01172
Catalytic activity	N(2)-succinyl-L-arginine + 2 H2O = N(2)-succinyl-L-ornithine + 2 NH3 + CO2. HAMAP MF_01172
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 2/5. HAMAP MF_01172
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the succinylarginine dihydrolase family.

Ontologies

Keywords
Biological process	Arginine metabolism
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP
Molecular function	N-succinylarginine dihydrolase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	N-succinylarginine dihydrolase HAMAP MF_01172		PRO_0000064716
Regions
	Region	19 – 28	10	Substrate binding By similarity
	Region	137 – 138	2	Substrate binding By similarity
Sites
	Active site	174	1	By similarity
	Active site	250	1	By similarity
	Active site	371	1	Nucleophile By similarity
	Binding site	110	1	Substrate By similarity
	Binding site	214	1	Substrate By similarity
	Binding site	252	1	Substrate By similarity
	Binding site	365	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O50175-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 39F9F379CCC16196	FASTA	448	48,875
	10 20 30 40 50 60 MNAHEVNFDG LVGPTHNYGG LSYGNVASQS NSQAVSNPKE AAKQGLAKMK ALMEMGFKQG 70 80 90 100 110 120 VLAPQARPDT AALRSLGFSG SDEEVIRRAA KEAMPLLAAC SSASSMWTAN AATVSPSADT 130 140 150 160 170 180 ADGRVHFTAA NLNCKFHRSI EHPTTSRVLA AMFNDERHFA HHAALPAVSQ FGDEGAANHT 190 200 210 220 230 240 RFCKDYGDAG VEFFVFGRSA FDSRFPAPQR YPARQTLEAC QAVARLHGLS EAGVVYAQQN 250 260 270 280 290 300 PAVIDQGVFH NDVISVGNGE VLFHHEDAFL DTEKVLAELH DKLGRRGGRF RAICVPRDQV 310 320 330 340 350 360 AVEDAVKSYL FNSQLLSKAD GSMLLVVPEE CRNNPRVWNY LDQLTGDDGP IREVKVFDLK 370 380 390 400 410 420 QSMQNGGGPA CLRLRVALQE RELAAVNPGV IMSAGLYDTL VAWVDRHYRD RLSETDLADP 430 440 QLLLECRTAL DELTQILKLG SVYSFQLD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the aru genes encoding enzymes of the catabolic arginine succinyltransferase pathway in Pseudomonas aeruginosa." Itoh Y. J. Bacteriol. 179:7280-7290(1997) [PubMed: 9393691] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF011922 Genomic DNA. Translation: AAC46013.1. AE004091 Genomic DNA. Translation: AAG04288.1.
PIR	D83533.
RefSeq	NP_249590.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	878588.
GenomeReviews	Gene locus PA0899 in contig AE004091_GR.
KEGG	pae:PA0899.
Organism-specific databases
PseudoCAP	PA0899.
CMR	Search...
Phylogenomic databases
HOGENOM	O50175.
OMA	O50175. KMKALME.
Enzyme and pathway databases
BioCyc	PAER208964:PA0899-MON.
BRENDA	3.5.3.23. 354.
Family and domain databases
HAMAP	MF_01172. [Tree]
InterPro	IPR007079. SuccinylArg_d-Hdrlase_AstB. [Graphical view]
Gene3D	G3DSA:3.75.10.20. SuccinylArg_di_hydro. 1 hit.
Pfam	PF04996. AstB. 1 hit. [Graphical view]
TIGRFAMs	TIGR03241. arg_catab_astB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASTB_PSEAE

Accession

Primary (citable) accession number: O50175

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)