ID   ASTD_PSEAE              Reviewed;         487 AA.
AC   O50174;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   28-NOV-2006, sequence version 2.
DT   16-JUN-2009, entry version 65.
DE   RecName: Full=N-succinylglutamate 5-semialdehyde dehydrogenase;
DE            EC=1.2.1.71;
DE   AltName: Full=Succinylglutamic semialdehyde dehydrogenase;
DE            Short=SGSD;
GN   Name=astD; Synonyms=aruD; OrderedLocusNames=PA0898;
OS   Pseudomonas aeruginosa.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, AND PATHWAY.
RC   STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
RX   MEDLINE=98053840; PubMed=9393691;
RA   Itoh Y.;
RT   "Cloning and characterization of the aru genes encoding enzymes of the
RT   catabolic arginine succinyltransferase pathway in Pseudomonas
RT   aeruginosa.";
RL   J. Bacteriol. 179:7280-7290(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
RX   MEDLINE=20437337; PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T.,
RA   Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an
RT   opportunistic pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Catalyzes the NAD-dependent reduction of
CC       succinylglutamate semialdehyde into succinylglutamate (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: N-succinyl-L-glutamate 5-semialdehyde + NAD(+)
CC       + H(2)O = N-succinyl-L-glutamate + NADH.
CC   -!- PATHWAY: Amino-acid degradation; L-arginine degradation via AST
CC       pathway; L-glutamate and succinate from L-arginine: step 4/5.
CC   -!- INDUCTION: By arginine, under the control of argR.
CC   -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family. AstD
CC       subfamily.
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DR   EMBL; AF011922; AAC46012.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AE004091; AAG04287.1; ALT_INIT; Genomic_DNA.
DR   PIR; C83533; C83533.
DR   RefSeq; NP_249589.1; -.
DR   GeneID; 883040; -.
DR   GenomeReviews; AE004091_GR; PA0898.
DR   KEGG; pae:PA0898; -.
DR   NMPDR; fig|208964.1.peg.899; -.
DR   PseudoCAP; PA0898; -.
DR   HOGENOM; O50174; -.
DR   OMA; O50174; EMTQPQA.
DR   BioCyc; PAER208964:PA0898-MON; -.
DR   BRENDA; 1.2.1.71; 354.
DR   GO; GO:0043824; F:succinylglutamate-semialdehyde dehydrogenas...; IEA:EC.
DR   GO; GO:0019544; P:arginine catabolic process to glutamate; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01174; -; 1.
DR   InterPro; IPR016160; Ald_DH_CS.
DR   InterPro; IPR015590; Aldehyde_DH.
DR   InterPro; IPR017649; SuccinylGlu_semiald_DH_AstD.
DR   PANTHER; PTHR11699; Aldehyde_dehyd; 1.
DR   Pfam; PF00171; Aldedh; 1.
DR   TIGRFAMs; TIGR03240; arg_catab_astD; 1.
DR   PROSITE; PS00070; ALDEHYDE_DEHYDR_CYS; 1.
DR   PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; 1.
PE   2: Evidence at transcript level;
KW   Arginine metabolism; Complete proteome; NAD; Oxidoreductase.
FT   CHAIN         1    487       N-succinylglutamate 5-semialdehyde
FT                                dehydrogenase.
FT                                /FTId=PRO_0000056570.
FT   NP_BIND     221    226       NAD (By similarity).
FT   ACT_SITE    244    244       By similarity.
FT   ACT_SITE    278    278       By similarity.
SQ   SEQUENCE   487 AA;  51380 MW;  79EA94BF49476DFD CRC64;
     MSTHYIAGQW LAGQGETLES LDPVGQGVVW SGRGADATQV DAAVCAAREA FPAWARRPLE
     QRIELLERFA ATLKSRADEL ARVIGEETGK PLWESATEVT SMVNKVAISV QAFRERTGEK
     SGPLADATAV LRHKPHGVVA VFGPYNFPGH LPNGHIVPAL LAGNCVVFKP SELTPKVAEL
     TLKAWIQAGL PAGVLNLVQG GRETGVALAA HRGLDGLFFT GSSRTGNLLH SQFGGQPQKI
     LALEMGGNNP LVVEEVADLD AAVYTIIQSA FISAGQRCTC ARRLLVPQGA WGDALLARLV
     AVSATLRVGR FDEQPAPFMG AVISLSAAEH LLKAQEHLIG KGAQPLLAMT QPIDGAALLT
     PGILDVSAVA ERPDEEFFGP LLQVIRYSDF AAAIREANAT QYGLAAGLLS DSRERFEQFL
     VESRAGIVNW NKQLTGAASS APFGGIGASG NHRPSAYYAA DYCAYPVASL ESPSVSLPAT
     LTPGISL
//