O50174 (ASTD_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-succinylglutamate 5-semialdehyde dehydrogenase
EC=1.2.1.71

Alternative name(s):
Succinylglutamic semialdehyde dehydrogenase
Short name=SGSD

Gene names

Name:	astD
Synonyms:	aruD
Ordered Locus Names:	PA0898

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate By similarity. HAMAP MF_01174
Catalytic activity	N-succinyl-L-glutamate 5-semialdehyde + NAD+ + H2O = N-succinyl-L-glutamate + NADH. HAMAP MF_01174
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 4/5. Ref.1
Induction	By arginine, under the control of ArgR. Ref.1
Sequence similarities	Belongs to the aldehyde dehydrogenase family. AstD subfamily.
Sequence caution	The sequence AAC46012.1 differs from that shown. Reason: Erroneous initiation. The sequence AAG04287.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	succinylglutamate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 487

487

N-succinylglutamate 5-semialdehyde dehydrogenase HAMAP MF_01174

PRO_0000056570

Regions

Nucleotide binding

221 – 226

6

NAD By similarity

Sites

Active site

244

1

By similarity

Active site

278

1

By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

487

Helix Strand Turn

Details...

Helix

37 – 56

20

Helix

59 – 75

17

Helix

77 – 83

7

Helix

92 – 116

25

Beta strand

127 – 135

9

Beta strand

139 – 142

4

Helix

156 – 161

6

Beta strand

166 – 168

3

Helix

175 – 187

13

Beta strand

195 – 197

3

Turn

207 – 210

4

Helix

228 – 232

5

Beta strand

249 – 253

5

Helix

259 – 271

13

Beta strand

280 – 287

8

Helix

294 – 305

12

Helix

325 – 338

14

Beta strand

344 – 347

4

Beta strand

383 – 389

7

Helix

390 – 398

9

Helix

413 – 422

10

Beta strand

430 – 432

3

Beta strand

441 – 443

3

Helix

459 – 463

5

Beta strand

464 – 471

8

Sequences

	Sequence		Length	Mass (Da)
	O50174 [UniParc]. Last modified November 28, 2006. Version 2. Checksum: 79EA94BF49476DFD	FASTA	487	51,380
	10 20 30 40 50 60 MSTHYIAGQW LAGQGETLES LDPVGQGVVW SGRGADATQV DAAVCAAREA FPAWARRPLE 70 80 90 100 110 120 QRIELLERFA ATLKSRADEL ARVIGEETGK PLWESATEVT SMVNKVAISV QAFRERTGEK 130 140 150 160 170 180 SGPLADATAV LRHKPHGVVA VFGPYNFPGH LPNGHIVPAL LAGNCVVFKP SELTPKVAEL 190 200 210 220 230 240 TLKAWIQAGL PAGVLNLVQG GRETGVALAA HRGLDGLFFT GSSRTGNLLH SQFGGQPQKI 250 260 270 280 290 300 LALEMGGNNP LVVEEVADLD AAVYTIIQSA FISAGQRCTC ARRLLVPQGA WGDALLARLV 310 320 330 340 350 360 AVSATLRVGR FDEQPAPFMG AVISLSAAEH LLKAQEHLIG KGAQPLLAMT QPIDGAALLT 370 380 390 400 410 420 PGILDVSAVA ERPDEEFFGP LLQVIRYSDF AAAIREANAT QYGLAAGLLS DSRERFEQFL 430 440 450 460 470 480 VESRAGIVNW NKQLTGAASS APFGGIGASG NHRPSAYYAA DYCAYPVASL ESPSVSLPAT LTPGISL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the aru genes encoding enzymes of the catabolic arginine succinyltransferase pathway in Pseudomonas aeruginosa." Itoh Y. J. Bacteriol. 179:7280-7290(1997) [PubMed: 9393691] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, PATHWAY. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF011922 Genomic DNA. Translation: AAC46012.1. Different initiation.
AE004091 Genomic DNA. Translation: AAG04287.1. Different initiation.

PIR

C83533.

RefSeq

NP_249589.1. NC_002516.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3JU8	X-ray	1.82	A/B	1-486	[»]

ProteinModelPortal

O50174.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

883040.

GenomeReviews

Gene locus PA0898 in contig AE004091_GR.

KEGG

pae:PA0898.

NMPDR

fig|208964.1.peg.899.

PATRIC

19836082. VBIPseAer58763_0933.

Organism-specific databases

PseudoCAP

PA0898.

Phylogenomic databases

HOGENOM

HBG752218.

OMA

MTQRDPR.

ProtClustDB

PRK09457.

Enzyme and pathway databases

BioCyc

PAER208964:PA0898-MONOMER.

Family and domain databases

HAMAP

MF_01174. Aldedh_AstD.
[Tree]

InterPro

IPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR017649. SuccinylGlu_semiald_DH_AstD.
[Graphical view]

Gene3D

G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit.
G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.

KO

K06447.

PANTHER

PTHR11699:SF24. PTHR11699:SF24. 1 hit.

Pfam

PF00171. Aldedh. 1 hit.
[Graphical view]

SUPFAM

SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.

TIGRFAMs

TIGR03240. Arg_catab_astD. 1 hit.

PROSITE

PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ASTD_PSEAE

Accession

Primary (citable) accession number: O50174

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 28, 2006
Last modified:	January 25, 2012
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program