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O50174 (ASTD_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N-succinylglutamate 5-semialdehyde dehydrogenase

EC=1.2.1.71
Alternative name(s):
Succinylglutamic semialdehyde dehydrogenase
Short name=SGSD
Gene names
Name:astD
Synonyms:aruD
Ordered Locus Names:PA0898
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifier208964 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length487 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate By similarity. HAMAP MF_01174

Catalytic activity

N-succinyl-L-glutamate 5-semialdehyde + NAD+ + H2O = N-succinyl-L-glutamate + NADH. HAMAP MF_01174

Pathway

Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 4/5. Ref.1

Induction

By arginine, under the control of ArgR. Ref.1

Sequence similarities

Belongs to the aldehyde dehydrogenase family. AstD subfamily.

Sequence caution

The sequence AAC46012.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAG04287.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processArginine metabolism
   LigandNAD
   Molecular functionOxidoreductase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological processarginine catabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular functionsuccinylglutamate-semialdehyde dehydrogenase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 487487N-succinylglutamate 5-semialdehyde dehydrogenase HAMAP MF_01174
PRO_0000056570

Regions

Nucleotide binding221 – 2266NAD By similarity

Sites

Active site2441 By similarity
Active site2781 By similarity

Secondary structure

................................................. 487
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O50174 [UniParc].

Last modified November 28, 2006. Version 2.
Checksum: 79EA94BF49476DFD

FASTA48751,380
        10         20         30         40         50         60 
MSTHYIAGQW LAGQGETLES LDPVGQGVVW SGRGADATQV DAAVCAAREA FPAWARRPLE 

        70         80         90        100        110        120 
QRIELLERFA ATLKSRADEL ARVIGEETGK PLWESATEVT SMVNKVAISV QAFRERTGEK 

       130        140        150        160        170        180 
SGPLADATAV LRHKPHGVVA VFGPYNFPGH LPNGHIVPAL LAGNCVVFKP SELTPKVAEL 

       190        200        210        220        230        240 
TLKAWIQAGL PAGVLNLVQG GRETGVALAA HRGLDGLFFT GSSRTGNLLH SQFGGQPQKI 

       250        260        270        280        290        300 
LALEMGGNNP LVVEEVADLD AAVYTIIQSA FISAGQRCTC ARRLLVPQGA WGDALLARLV 

       310        320        330        340        350        360 
AVSATLRVGR FDEQPAPFMG AVISLSAAEH LLKAQEHLIG KGAQPLLAMT QPIDGAALLT 

       370        380        390        400        410        420 
PGILDVSAVA ERPDEEFFGP LLQVIRYSDF AAAIREANAT QYGLAAGLLS DSRERFEQFL 

       430        440        450        460        470        480 
VESRAGIVNW NKQLTGAASS APFGGIGASG NHRPSAYYAA DYCAYPVASL ESPSVSLPAT 


LTPGISL 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of the aru genes encoding enzymes of the catabolic arginine succinyltransferase pathway in Pseudomonas aeruginosa."
Itoh Y.
J. Bacteriol. 179:7280-7290(1997) [PubMed: 9393691] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, PATHWAY.
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen."
Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. expand/collapse author list , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.
Nature 406:959-964(2000) [PubMed: 10984043] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF011922 Genomic DNA. Translation: AAC46012.1. Different initiation.
AE004091 Genomic DNA. Translation: AAG04287.1. Different initiation.
PIRC83533.
RefSeqNP_249589.1. NC_002516.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3JU8X-ray1.82A/B1-486[»]
ProteinModelPortalO50174.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID883040.
GenomeReviewsGene locus PA0898 in contig AE004091_GR.
KEGGpae:PA0898.
NMPDRfig|208964.1.peg.899.
PATRIC19836082. VBIPseAer58763_0933.

Organism-specific databases

PseudoCAPPA0898.

Phylogenomic databases

HOGENOMHBG752218.
OMAMTQRDPR.
ProtClustDBPRK09457.

Enzyme and pathway databases

BioCycPAER208964:PA0898-MONOMER.

Family and domain databases

HAMAPMF_01174. Aldedh_AstD.
[Tree]
InterProIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR017649. SuccinylGlu_semiald_DH_AstD.
[Graphical view]
Gene3DG3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit.
G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
KOK06447.
PANTHERPTHR11699:SF24. PTHR11699:SF24. 1 hit.
PfamPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMSSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMsTIGR03240. Arg_catab_astD. 1 hit.
PROSITEPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASTD_PSEAE
AccessionPrimary (citable) accession number: O50174
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 28, 2006
Last modified: January 25, 2012
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families