Reviewed, UniProtKB/Swiss-Prot O50174 (ASTD_PSEAE)

Names and origin

Protein names

Recommended name:
    N-succinylglutamate 5-semialdehyde dehydrogenase
    EC=1.2.1.71
Alternative name(s):
    Succinylglutamic semialdehyde dehydrogenase
      Short name=SGSD

Gene names

Name:	astD
Synonyms:	aruD
Ordered Locus Names:	PA0898

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate By similarity.
Catalytic activity	N-succinyl-L-glutamate 5-semialdehyde + NAD(+) + H(2)O = N-succinyl-L-glutamate + NADH.
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 4/5.
Induction	By arginine, under the control of argR.
Sequence similarities	Belongs to the aldehyde dehydrogenase family. AstD subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	succinylglutamate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 487	487	N-succinylglutamate 5-semialdehyde dehydrogenase		PRO_0000056570
Regions
	Nucleotide binding	221 – 226	6	NAD By similarity
Sites
	Active site	244	1	By similarity
	Active site	278	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O50174-1 [UniParc]. Last modified November 28, 2006. Version 2. Checksum: 79EA94BF49476DFD	FASTA	487	51,380
	10 20 30 40 50 60 MSTHYIAGQW LAGQGETLES LDPVGQGVVW SGRGADATQV DAAVCAAREA FPAWARRPLE 70 80 90 100 110 120 QRIELLERFA ATLKSRADEL ARVIGEETGK PLWESATEVT SMVNKVAISV QAFRERTGEK 130 140 150 160 170 180 SGPLADATAV LRHKPHGVVA VFGPYNFPGH LPNGHIVPAL LAGNCVVFKP SELTPKVAEL 190 200 210 220 230 240 TLKAWIQAGL PAGVLNLVQG GRETGVALAA HRGLDGLFFT GSSRTGNLLH SQFGGQPQKI 250 260 270 280 290 300 LALEMGGNNP LVVEEVADLD AAVYTIIQSA FISAGQRCTC ARRLLVPQGA WGDALLARLV 310 320 330 340 350 360 AVSATLRVGR FDEQPAPFMG AVISLSAAEH LLKAQEHLIG KGAQPLLAMT QPIDGAALLT 370 380 390 400 410 420 PGILDVSAVA ERPDEEFFGP LLQVIRYSDF AAAIREANAT QYGLAAGLLS DSRERFEQFL 430 440 450 460 470 480 VESRAGIVNW NKQLTGAASS APFGGIGASG NHRPSAYYAA DYCAYPVASL ESPSVSLPAT LTPGISL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the aru genes encoding enzymes of the catabolic arginine succinyltransferase pathway in Pseudomonas aeruginosa." Itoh Y. J. Bacteriol. 179:7280-7290(1997) [PubMed: 9393691] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, PATHWAY. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF011922 Genomic DNA. Translation: AAC46012.1. Different initiation. AE004091 Genomic DNA. Translation: AAG04287.1. Different initiation.
PIR	C83533.
RefSeq	NP_249589.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	883040.
GenomeReviews	Gene locus PA0898 in contig AE004091_GR.
KEGG	pae:PA0898.
NMPDR	fig|208964.1.peg.899.
Organism-specific databases
PseudoCAP	PA0898.
CMR	Search...
Phylogenomic databases
HOGENOM	O50174.
Enzyme and pathway databases
BioCyc	PAER208964:PA0898-MON.
Family and domain databases
HAMAP	MF_01174. [Tree]
InterPro	IPR016160. Ald_DHase_CS. IPR015590. Aldehyde_DHase. IPR017649. SuccinylGlu_semiald_DH_AstD. [Graphical view]
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASTD_PSEAE

Accession

Primary (citable) accession number: O50174

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 28, 2006
Last modified:	November 25, 2008
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)