Reviewed, UniProtKB/Swiss-Prot O50146 (ARGC2_THET2)

Names and origin

Protein names

Recommended name:
    N-acetyl-gamma-glutamyl-phosphate/N-acetyl-gamma-aminoadipyl-phosphate reductase
      Short name=AGPR
    EC=1.2.1.38
    EC=1.2.1.-
Alternative name(s):
    N-acetyl-glutamate semialdehyde/N-acetyl-aminoadipate semialdehyde dehydrogenase
      Short name=NAGSA dehydrogenase

Gene names

Name:	argC2
Synonyms:	argC, lysY
Ordered Locus Names:	TT_C1542

Organism

Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) [Complete proteome] [HAMAP]

Taxonomic identifier

262724 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in both the arginine and lysine biosynthetic pathways By similarity.
Catalytic activity	N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate = N-acetyl-5-glutamyl phosphate + NADPH. HAMAP MF_00150 N(2)-acetyl-L-aminoadipate-semialdehyde + NADP+ + phosphate = N(2)-acetyl-L-gamma-aminoadipyl phosphate + NADPH. HAMAP MF_00150
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 3/4. HAMAP MF_00150 Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (Thermus route): step 3/5. HAMAP MF_00150
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the NAGSA dehydrogenase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP lysine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetyl-gamma-glutamyl-phosphate reductase activity Inferred from electronic annotation. Source: HAMAP NAD or NADH binding Inferred from electronic annotation. Source: InterPro protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	N-acetyl-gamma-glutamyl-phosphate/N-acetyl-gamma-aminoadipyl-phosphate reductase HAMAP MF_00150		PRO_0000112501
Sites
	Active site	148	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O50146-1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: B11CE66CB2F04C15	FASTA	344	38,051
	10 20 30 40 50 60 MDKKTLSIVG ASGYAGGEFL RLALSHPYLE VKQVTSRRFA GEPVHFVHPN LRGRTNLKFI 70 80 90 100 110 120 PPEKLEPADI LVLALPHGVF AREFDRYSAL APILIDLSAD FRLKDPELYR RYYGEHPRPD 130 140 150 160 170 180 LLGCFVYAVP ELYREALKGA DWIAGAGCNA TATLLGLYPL LKAGVLKPTP IFVTLLISTS 190 200 210 220 230 240 AAGAEASPAS HHPERAGSIR VYKPTGHRHT AEVVENLPGR PEVHLTAIAT DRVRGILMTA 250 260 270 280 290 300 QCFVQDGWSE RDVWQAYREA YAGEPFIRLV KQKKGVHRYP DPRFVQGTNY ADIGFELEED 310 320 330 340 TGRLVVMTAI DNLVKGTAGH ALQALNVRMG WPETLGLDFP GLHP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of the extreme thermophile Thermus thermophilus." Henne A., Brueggemann H., Raasch C., Wiezer A., Hartsch T., Liesegang H., Johann A., Lienard T., Gohl O., Martinez-Arias R., Jacobi C., Starkuviene V., Schlenczeck S., Dencker S., Huber R., Klenk H.-P., Kramer W., Merkl R., Gottschalk G., Fritz H.-J. Nat. Biotechnol. 22:547-553(2004) [PubMed: 15064768] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Aspartate kinase-independent lysine synthesis in an extremely thermophilic bacterium, Thermus thermophilus: lysine is synthesized via alpha-aminoadipic acid not via diaminopimelic acid." Kobashi N., Nishiyama M., Tanokura M. J. Bacteriol. 181:1713-1718(1999) [PubMed: 10074061] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-236.
[3]	"Molecular cloning and sequence analysis of the lysR gene from the extremely thermophilic eubacterium, Thermus thermophilus HB27." Kosuge T., Hoshino T. FEMS Microbiol. Lett. 157:73-79(1997) [PubMed: 9418242] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 35-344.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017221 Genomic DNA. Translation: AAS81884.1. AB017109 mRNA. Translation: BAA74767.1. AB006681 Genomic DNA. Translation: BAA23878.1. Different initiation.
PIR	T43947.
RefSeq	YP_005511.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2776302.
GenomeReviews	Gene locus TT_C1542 in contig AE017221_GR.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O50146.
OMA	O50146. GCNATAT.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-6802. TTHE262724:TT_C1542-MON.
Family and domain databases
HAMAP	MF_00150. [Tree]
InterPro	IPR000706. AGPR_act_site. IPR000534. Semialdehyde_DH_NAD-bd. IPR012280. Semialdhyde_DH_C. [Graphical view]
Pfam	PF01118. Semialdhyde_dh. 1 hit. PF02774. Semialdhyde_dhC. 1 hit. [Graphical view]
ProDom	PD003765. AGPR_act_site. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01850. argC. 1 hit.
PROSITE	PS01224. ARGC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGC2_THET2

Accession

Primary (citable) accession number: O50146
Secondary accession number(s): Q9ZND6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	June 16, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)