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Protein

Phosphoglycolate phosphatase

Gene

PH1421

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the dephosphorylation of 2-phosphoglycolate (By similarity). Has phosphatase activity towards p-nitrophenylphosphate (in vitro).UniRule annotation

Catalytic activityi

2-phosphoglycolate + H2O = glycolate + phosphate.UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei9NucleophileUniRule annotation1
Metal bindingi9MagnesiumUniRule annotation1
Active sitei11Proton donorBy similarity1
Metal bindingi11MagnesiumUniRule annotation1
Metal bindingi11Magnesium; via carbonyl oxygenUniRule annotation1
Binding sitei154SubstrateUniRule annotation1
Metal bindingi177MagnesiumUniRule annotation1
Metal bindingi181MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1420-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycolate phosphataseUniRule annotation (EC:3.1.3.18UniRule annotation)
Short name:
PGPUniRule annotation
Short name:
PGPaseUniRule annotation
Gene namesi
Ordered Locus Names:PH1421
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001467251 – 231Phosphoglycolate phosphataseAdd BLAST231

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi70601.PH1421.

Structurei

Secondary structure

1231
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 10Combined sources6
Turni11 – 13Combined sources3
Helixi23 – 34Combined sources12
Beta strandi39 – 42Combined sources4
Helixi47 – 57Combined sources11
Beta strandi63 – 65Combined sources3
Helixi66 – 68Combined sources3
Beta strandi70 – 73Combined sources4
Beta strandi76 – 80Combined sources5
Helixi85 – 96Combined sources12
Helixi106 – 108Combined sources3
Beta strandi113 – 115Combined sources3
Turni117 – 119Combined sources3
Helixi122 – 131Combined sources10
Beta strandi137 – 140Combined sources4
Beta strandi145 – 148Combined sources4
Helixi154 – 165Combined sources12
Helixi169 – 171Combined sources3
Beta strandi172 – 176Combined sources5
Helixi179 – 181Combined sources3
Helixi182 – 187Combined sources6
Beta strandi188 – 193Combined sources6
Helixi199 – 202Combined sources4
Beta strandi206 – 208Combined sources3
Helixi213 – 227Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WR8X-ray1.60A/B1-231[»]
ProteinModelPortaliO50129.
SMRiO50129.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO50129.

Family & Domainsi

Sequence similaritiesi

Belongs to the archaeal SPP-like hydrolase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01213. Archaea.
COG0561. LUCA.
HOGENOMiHOG000184780.
OMAiMPERKAG.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
HAMAPiMF_01419. GPH_hydrolase_arch. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR006382. PGPase.
[Graphical view]
PfamiPF08282. Hydrolase_3. 2 hits.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.

Sequencei

Sequence statusi: Complete.

O50129-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIKAISIDI DGTITYPNRM IHEKALEAIR RAESLGIPIM LVTGNTVQFA
60 70 80 90 100
EAASILIGTS GPVVAEDGGA ISYKKKRIFL ASMDEEWILW NEIRKRFPNA
110 120 130 140 150
RTSYTMPDRR AGLVIMRETI NVETVREIIN ELNLNLVAVD SGFAIHVKKP
160 170 180 190 200
WINKGSGIEK ASEFLGIKPK EVAHVGDGEN DLDAFKVVGY KVAVAQAPKI
210 220 230
LKENADYVTK KEYGEGGAEA IYHILEKFGY L
Length:231
Mass (Da):25,606
Last modified:June 1, 1998 - v1
Checksum:iAAE2AEB8733E14A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30527.1.
PIRiG71015.
RefSeqiWP_010885503.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30527; BAA30527; BAA30527.
GeneIDi1443741.
KEGGipho:PH1421.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30527.1.
PIRiG71015.
RefSeqiWP_010885503.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WR8X-ray1.60A/B1-231[»]
ProteinModelPortaliO50129.
SMRiO50129.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1421.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30527; BAA30527; BAA30527.
GeneIDi1443741.
KEGGipho:PH1421.

Phylogenomic databases

eggNOGiarCOG01213. Archaea.
COG0561. LUCA.
HOGENOMiHOG000184780.
OMAiMPERKAG.

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1420-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO50129.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
HAMAPiMF_01419. GPH_hydrolase_arch. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR006382. PGPase.
[Graphical view]
PfamiPF08282. Hydrolase_3. 2 hits.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPGP_PYRHO
AccessioniPrimary (citable) accession number: O50129
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: June 1, 1998
Last modified: November 2, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.