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Protein

Phosphoglycolate phosphatase

Gene

PH1421

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the dephosphorylation of 2-phosphoglycolate (By similarity). Has phosphatase activity towards p-nitrophenylphosphate (in vitro).UniRule annotation

Catalytic activityi

2-phosphoglycolate + H2O = glycolate + phosphate.UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei9 – 91NucleophileUniRule annotation
Metal bindingi9 – 91MagnesiumUniRule annotation
Active sitei11 – 111Proton donorBy similarity
Metal bindingi11 – 111MagnesiumUniRule annotation
Metal bindingi11 – 111Magnesium; via carbonyl oxygenUniRule annotation
Binding sitei154 – 1541SubstrateUniRule annotation
Metal bindingi177 – 1771MagnesiumUniRule annotation
Metal bindingi181 – 1811MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1421-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycolate phosphataseUniRule annotation (EC:3.1.3.18UniRule annotation)
Short name:
PGPUniRule annotation
Short name:
PGPaseUniRule annotation
Gene namesi
Ordered Locus Names:PH1421
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 231231Phosphoglycolate phosphatasePRO_0000146725Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi70601.PH1421.

Structurei

Secondary structure

1
231
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 106Combined sources
Turni11 – 133Combined sources
Helixi23 – 3412Combined sources
Beta strandi39 – 424Combined sources
Helixi47 – 5711Combined sources
Beta strandi63 – 653Combined sources
Helixi66 – 683Combined sources
Beta strandi70 – 734Combined sources
Beta strandi76 – 805Combined sources
Helixi85 – 9612Combined sources
Helixi106 – 1083Combined sources
Beta strandi113 – 1153Combined sources
Turni117 – 1193Combined sources
Helixi122 – 13110Combined sources
Beta strandi137 – 1404Combined sources
Beta strandi145 – 1484Combined sources
Helixi154 – 16512Combined sources
Helixi169 – 1713Combined sources
Beta strandi172 – 1765Combined sources
Helixi179 – 1813Combined sources
Helixi182 – 1876Combined sources
Beta strandi188 – 1936Combined sources
Helixi199 – 2024Combined sources
Beta strandi206 – 2083Combined sources
Helixi213 – 22715Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WR8X-ray1.60A/B1-231[»]
ProteinModelPortaliO50129.
SMRiO50129. Positions 1-231.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO50129.

Family & Domainsi

Sequence similaritiesi

Belongs to the archaeal SPP-like hydrolase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01213. Archaea.
COG0561. LUCA.
HOGENOMiHOG000184780.
KOiK07024.
OMAiMPERKAG.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
HAMAPiMF_01419. GPH_hydrolase_arch. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR006382. PGPase.
[Graphical view]
PfamiPF08282. Hydrolase_3. 2 hits.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.

Sequencei

Sequence statusi: Complete.

O50129-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIKAISIDI DGTITYPNRM IHEKALEAIR RAESLGIPIM LVTGNTVQFA
60 70 80 90 100
EAASILIGTS GPVVAEDGGA ISYKKKRIFL ASMDEEWILW NEIRKRFPNA
110 120 130 140 150
RTSYTMPDRR AGLVIMRETI NVETVREIIN ELNLNLVAVD SGFAIHVKKP
160 170 180 190 200
WINKGSGIEK ASEFLGIKPK EVAHVGDGEN DLDAFKVVGY KVAVAQAPKI
210 220 230
LKENADYVTK KEYGEGGAEA IYHILEKFGY L
Length:231
Mass (Da):25,606
Last modified:June 1, 1998 - v1
Checksum:iAAE2AEB8733E14A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30527.1.
PIRiG71015.
RefSeqiWP_010885503.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30527; BAA30527; BAA30527.
GeneIDi1443741.
KEGGipho:PH1421.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30527.1.
PIRiG71015.
RefSeqiWP_010885503.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WR8X-ray1.60A/B1-231[»]
ProteinModelPortaliO50129.
SMRiO50129. Positions 1-231.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1421.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30527; BAA30527; BAA30527.
GeneIDi1443741.
KEGGipho:PH1421.

Phylogenomic databases

eggNOGiarCOG01213. Archaea.
COG0561. LUCA.
HOGENOMiHOG000184780.
KOiK07024.
OMAiMPERKAG.

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1421-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO50129.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
HAMAPiMF_01419. GPH_hydrolase_arch. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR006382. PGPase.
[Graphical view]
PfamiPF08282. Hydrolase_3. 2 hits.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPGP_PYRHO
AccessioniPrimary (citable) accession number: O50129
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: June 1, 1998
Last modified: April 13, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.