Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Flap endonuclease 1

Gene

fen

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA (By similarity).By similarity

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi27Magnesium 1UniRule annotation1
Metal bindingi80Magnesium 1UniRule annotation1
Metal bindingi152Magnesium 1UniRule annotation1
Metal bindingi154Magnesium 1UniRule annotation1
Metal bindingi173Magnesium 2UniRule annotation1
Metal bindingi175Magnesium 2UniRule annotation1
Metal bindingi236Magnesium 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Exonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair, DNA replication
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciPHOR70601:G1G39-1375-MONOMER
BRENDAi3.1.99.B1 5244

Names & Taxonomyi

Protein namesi
Recommended name:
Flap endonuclease 1UniRule annotation (EC:3.1.-.-UniRule annotation)
Short name:
FEN-1UniRule annotation
Alternative name(s):
Flap structure-specific endonuclease 1UniRule annotation
Gene namesi
Name:fenUniRule annotation
Ordered Locus Names:PH1415
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001540621 – 343Flap endonuclease 1Add BLAST343

Interactioni

Subunit structurei

Interacts with PCNA. PCNA stimulates the nuclease activity without altering cleavage specificity.UniRule annotation

Protein-protein interaction databases

STRINGi70601.PH1415

Structurei

Secondary structure

1343
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 8Combined sources4
Turni16 – 21Combined sources6
Beta strandi23 – 27Combined sources5
Helixi28 – 38Combined sources11
Beta strandi39 – 41Combined sources3
Turni42 – 44Combined sources3
Helixi56 – 71Combined sources16
Beta strandi74 – 79Combined sources6
Beta strandi90 – 93Combined sources4
Beta strandi102 – 107Combined sources6
Helixi109 – 111Combined sources3
Turni112 – 114Combined sources3
Turni117 – 124Combined sources8
Helixi129 – 142Combined sources14
Helixi153 – 161Combined sources9
Turni162 – 164Combined sources3
Helixi175 – 178Combined sources4
Beta strandi182 – 186Combined sources5
Helixi188 – 190Combined sources3
Beta strandi208 – 211Combined sources4
Helixi212 – 217Combined sources6
Turni218 – 220Combined sources3
Helixi223 – 233Combined sources11
Helixi247 – 255Combined sources9
Beta strandi256 – 259Combined sources4
Helixi261 – 263Combined sources3
Turni265 – 268Combined sources4
Helixi273 – 280Combined sources8
Helixi297 – 304Combined sources8
Turni305 – 308Combined sources4
Helixi312 – 327Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MC8X-ray3.10A/B1-343[»]
ProteinModelPortaliO50123
SMRiO50123
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO50123

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 98N-domainAdd BLAST98
Regioni116 – 258I-domainAdd BLAST143
Regioni330 – 338Interaction with PCNAUniRule annotation9

Sequence similaritiesi

Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04050 Archaea
COG0258 LUCA
HOGENOMiHOG000193852
KOiK04799
OMAiGSQDYDS
OrthoDBiPOG093Z0642

Family and domain databases

CDDicd09867 PIN_FEN1, 1 hit
HAMAPiMF_00614 Fen, 1 hit
InterProiView protein in InterPro
IPR036279 5-3_exonuclease_C_sf
IPR023426 Flap_endonuc
IPR019973 Flap_structure-sp_endonuc_arc
IPR008918 HhH2
IPR029060 PIN-like_dom_sf
IPR006086 XPG-I_dom
IPR006084 XPG/Rad2
IPR019974 XPG_CS
IPR006085 XPG_DNA_repair_N
PANTHERiPTHR11081 PTHR11081, 1 hit
PfamiView protein in Pfam
PF00867 XPG_I, 1 hit
PF00752 XPG_N, 1 hit
PRINTSiPR00853 XPGRADSUPER
SMARTiView protein in SMART
SM00279 HhH2, 1 hit
SM00484 XPGI, 1 hit
SM00485 XPGN, 1 hit
SUPFAMiSSF47807 SSF47807, 1 hit
SSF88723 SSF88723, 1 hit
TIGRFAMsiTIGR03674 fen_arch, 1 hit
PROSITEiView protein in PROSITE
PS00841 XPG_1, 1 hit

Sequencei

Sequence statusi: Complete.

O50123-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVPIGDLVP RKEIDLENLY GKKIAIDALN AIYQFLSTIR QRDGTPLMDS
60 70 80 90 100
KGRITSHLSG LFYRTINLME AGIKPAYVFD GKPPEFKRKE LEKRREAREE
110 120 130 140 150
AELKWKEALA KGNLEEARKY AQRATKVNEM LIEDAKKLLQ LMGIPIIQAP
160 170 180 190 200
SEGEAQAAYM ASKGDVYASA SQDYDSLLFG APRLIRNLTI TGKRKMPGKD
210 220 230 240 250
VYVEIKPELV VLDEVLKELK ITREKLIELA ILVGTDYNPG GVKGIGPKKA
260 270 280 290 300
LEIVRYSRDP LAKFQRQSDV DLYAIKEFFL NPPVTNEYSL SWKEPDEEGI
310 320 330 340
LKFLCDEHNF SEERVKNGIE RLKKAIKAGR QSTLESWFVK KKP
Length:343
Mass (Da):38,947
Last modified:June 1, 1998 - v1
Checksum:i8BE0025F372C3138
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA30521.1
PIRiA71015
RefSeqiWP_010885498.1, NC_000961.1

Genome annotation databases

EnsemblBacteriaiBAA30521; BAA30521; BAA30521
GeneIDi1443736
KEGGipho:PH1415

Similar proteinsi

Entry informationi

Entry nameiFEN_PYRHO
AccessioniPrimary (citable) accession number: O50123
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: June 1, 1998
Last modified: April 25, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health