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Protein

Putative 2-dehydropantoate 2-reductase

Gene

PH1390

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.By similarity

Catalytic activityi

(R)-pantoate + NADP+ = 2-dehydropantoate + NADPH.

Pathwayi: (R)-pantothenate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB)
  2. Putative 2-dehydropantoate 2-reductase (PH1390)
This subpathway is part of the pathway (R)-pantothenate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate, the pathway (R)-pantothenate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei99NADP; via amide nitrogenBy similarity1
Binding sitei99SubstrateBy similarity1
Active sitei179Proton donorBy similarity1
Binding sitei183SubstrateBy similarity1
Binding sitei187SubstrateBy similarity1
Binding sitei249SubstrateBy similarity1
Binding sitei261NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi7 – 12NADPBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processPantothenate biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciPHOR70601:G1G39-1355-MONOMER
UniPathwayiUPA00028; UER00004

Names & Taxonomyi

Protein namesi
Recommended name:
Putative 2-dehydropantoate 2-reductase (EC:1.1.1.169)
Alternative name(s):
Ketopantoate reductase
Short name:
KPA reductase
Short name:
KPR
Gene namesi
Ordered Locus Names:PH1390
ORF Names:PHAA019
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001573261 – 301Putative 2-dehydropantoate 2-reductaseAdd BLAST301

Interactioni

Protein-protein interaction databases

STRINGi70601.PH1390

Structurei

3D structure databases

ProteinModelPortaliO50098
SMRiO50098
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ketopantoate reductase family.Curated

Phylogenomic databases

eggNOGiarCOG04139 Archaea
COG1893 LUCA
HOGENOMiHOG000050222
KOiK00077
OMAiLTVLHDC
OrthoDBiPOG093Z0891

Family and domain databases

Gene3Di1.10.1040.10, 1 hit
InterProiView protein in InterPro
IPR008927 6-PGluconate_DH-like_C_sf
IPR013328 6PGD_dom2
IPR003710 ApbA
IPR013752 KPA_reductase
IPR013332 KPR_N
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02558 ApbA, 1 hit
PF08546 ApbA_C, 1 hit
SUPFAMiSSF48179 SSF48179, 1 hit
SSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00745 apbA_panE, 1 hit

Sequencei

Sequence statusi: Complete.

O50098-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIYILGAGA IGSLVGGLLA NVGEDVTLIG RGRHIEAINK RGLMIEGLTN
60 70 80 90 100
LKINTKATTS IPGAKPDLII LTTKSYSTDD ALNSAKDIVR DTWVLSLQNG
110 120 130 140 150
IGNEEKIMEL GGRPIGGITT NGAVLKEPGV VEWRGRGITL IGLYPKGRNE
160 170 180 190 200
FVEEVKETFN RAGLETEVTE NIIGWKWAKT IVNSAINPIG AILEVKNGAI
210 220 230 240 250
KDNDYLLSIA VEVVKEGCKI ALQNGIKFDI SPMELLIQTL EQTRENYNSM
260 270 280 290 300
LQDIWRGKRT EIDFINGKII EYAKLVNLEA PLNFLLWALV KAKESLGGGS

K
Length:301
Mass (Da):32,811
Last modified:June 1, 1998 - v1
Checksum:iE2354787F603880C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA30496.1
PIRiH71011
RefSeqiWP_010885478.1, NC_000961.1

Genome annotation databases

EnsemblBacteriaiBAA30496; BAA30496; BAA30496
GeneIDi1443716
KEGGipho:PH1390

Similar proteinsi

Entry informationi

Entry nameiPANE_PYRHO
AccessioniPrimary (citable) accession number: O50098
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: April 25, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health