Reviewed, UniProtKB/Swiss-Prot O50083 (RPIA_PYRHO)

Names and originHide

Protein names

Recommended name:
Ribose-5-phosphate isomerase A
EC=5.3.1.6

Alternative name(s):
Phosphoriboisomerase A
Short name=PRI

Gene names

Name:	rpiA
Ordered Locus Names:	PH1375

Organism

Pyrococcus horikoshii [Complete proteome] [HAMAP]

Taxonomic identifier

53953 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributesHide

Sequence length	229 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)Hide

Catalytic activity	D-ribose 5-phosphate = D-ribulose 5-phosphate. HAMAP MF_00170
Pathway	Carbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1. HAMAP MF_00170
Subunit structure	Homotetramer. HAMAP MF_00170
Sequence similarities	Belongs to the ribose 5-phosphate isomerase family.
Biophysicochemical properties	Temperature dependence: Optimum temperature is over 90 degrees Celsius. HAMAP MF_00170

OntologiesHide

Keywords
Molecular function	Isomerase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	pentose-phosphate shunt, non-oxidative branch Inferred from electronic annotation. Source: HAMAP
Molecular function	ribose-5-phosphate isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)Hide

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 229

229

Ribose-5-phosphate isomerase A HAMAP MF_00170

PRO_0000158517

Secondary structure

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229

Helix Strand Turn

Details...

Helix

3 – 14

12

Helix

15 – 17

3

Beta strand

23 – 26

4

Helix

30 – 44

15

Beta strand

52 – 57

6

Helix

58 – 66

9

Helix

74 – 76

3

Beta strand

80 – 85

6

Beta strand

88 – 90

3

Helix

104 – 112

9

Beta strand

114 – 122

9

Helix

123 – 125

3

Beta strand

136 – 140

5

Helix

142 – 144

3

Helix

145 – 151

7

Helix

152 – 155

4

Beta strand

158 – 161

4

Beta strand

165 – 170

6

Beta strand

178 – 183

6

Helix

190 – 198

9

Beta strand

203 – 209

7

Beta strand

215 – 220

6

Beta strand

223 – 228

6

SequencesHide

	Sequence		Length	Mass (Da)
	O50083-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 0E35BCB9F608D93C	FASTA	229	25,162
	10 20 30 40 50 60 MNVEEMKKIA AKEALKFIED DMVIGLGTGS TTAYFIKLLG EKLKRGEISD IVGVPTSYQA 70 80 90 100 110 120 KLLAIEHDIP IASLDQVDAI DVAVDGADEV DPNLNLIKGR GAALTMEKII EYRAGTFIVL 130 140 150 160 170 180 VDERKLVDYL CQKMPVPIEV IPQAWKAIIE ELSIFNAKAE LRMGVNKDGP VITDNGNFII 190 200 210 220 DAKFPRIDDP LDMEIELNTI PGVIENGIFA DIADIVIVGT REGVKKLER

ReferencesHide

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OT3.
[2]	"A hyperthermostable D-ribose-5-phosphate isomerase from Pyrococcus horikoshii characterization and three-dimensional structure." Ishikawa K., Matsui I., Payan F., Cambillau C., Ishida H., Kawarabayasi Y., Kikuchi H., Roussel A. Structure 10:877-886(2002) [PubMed: 12057201] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS), CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-referencesHide

Sequence databases

EMBL
GenBank
DDBJ

BA000001 Genomic DNA. Translation: BAA30481.1.

PIR

A71010.

RefSeq

NP_143254.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1LK5	X-ray	1.75	A/B/C/D	1-229	[»]
1LK7	X-ray	2.00	A/B/C/D	1-229	[»]

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-1502514.

Genome annotation databases

EnsemblBacteria

EBPYRT00000000157; EBPYRP00000000157; EBPYRG00000000157.

GeneID

1443702.

GenomeReviews

Gene locus PH1375 in contig BA000001_GR.

KEGG

pho:PH1375.

NMPDR

fig|70601.1.peg.1355.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

HBG515603.

OMA

VFDLNSV.

ProtClustDB

PRK00702.

Enzyme and pathway databases

BioCyc

PHOR70601:PH1375-MONOMER.

BRENDA

5.3.1.6. 74679.

Family and domain databases

HAMAP

MF_00170. Rib_5P_isom_A.
[Tree]

InterPro

IPR014036. HTH_DeoR.
IPR004788. Ribose5P_isomerase_typA.
IPR020672. Ribose5P_isomerase_typA_subgr.
[Graphical view]

PANTHER

PTHR11934. RpiA. 1 hit.

Pfam

PF00455. DeoR. 1 hit.
PF06026. Rib_5-P_isom_A. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00021. rpiA. 1 hit.

ProtoNet

Search...

Entry informationHide

Entry name

RPIA_PYRHO

Accession

Primary (citable) accession number: O50083

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	June 1, 1998
Last modified:	June 15, 2010
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)