SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O50078

- ACEA_HYPME

UniProt

O50078 - ACEA_HYPME

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Isocitrate lyase
Gene
aceA, icl
Organism
Hyphomicrobium methylovorum
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase-positive serine pathway.1 Publication

Catalytic activityi

Isocitrate = succinate + glyoxylate.1 Publication

Cofactori

Divalent cations. Magnesium and, to a lesser extent, manganese.1 Publication

Enzyme regulationi

In the presence of magnesium, inhibited by oxalate, potassium cyanide, manganese, silver, cadmium and to a lesser extent by succinate, glycolate, iodoacetamide, DL-penicillamine, aluminum, sodium, potassium, lithium and strontium.1 Publication

Kineticsi

  1. KM=0.51 mM for D-isocitrate1 Publication

pH dependencei

Optimum pH is 7.5. The enzyme is stable when incubated for 15 min at 30 degrees Celsius at pH 7.5-9.1 Publication

Temperature dependencei

Optimum temperature is 45 degrees Celsius. Loss of activity is 0%, 16%, 30%, 82% and 100% when incubated at 25, 30, 40, 50 and 60 degrees Celsius for 30 min, respectively.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei225 – 2251 By similarityBy similarity

GO - Molecular functioni

  1. isocitrate lyase activity Source: UniProtKB
  2. magnesium ion binding Source: UniProtKB
  3. transition metal ion binding Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB
  2. tricarboxylic acid cycle Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-5154-MONOMER.
UniPathwayiUPA00703; UER00719.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate lyase (EC:4.1.3.1)
Short name:
ICL
Short name:
Isocitrase
Short name:
Isocitratase
Gene namesi
Name:aceA
Synonyms:icl
OrganismiHyphomicrobium methylovorum
Taxonomic identifieri84 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesHyphomicrobiaceaeHyphomicrobium

Subcellular locationi

Cytoplasm Inferred

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 540539Isocitrate lyase1 Publication
PRO_0000389621Add
BLAST

Proteomic databases

PRIDEiO50078.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO50078.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.60. 3 hits.
InterProiIPR006254. Isocitrate_lyase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamiPF00463. ICL. 3 hits.
[Graphical view]
PIRSFiPIRSF001362. Isocit_lyase. 1 hit.
SUPFAMiSSF51621. SSF51621. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O50078-1 [UniParc]FASTAAdd to Basket

« Hide

MAHKKTYSQL RSELLARYPV GLTKGGVSID DIVQLRLQSP YESHLDVARA    50
MASVMRADMA AYDRDTGKFT QSLGCWSGFH AQQMIKAVKR LRGTTKGAYV 100
YLSGWMVAGL RNRWGHLPDQ SMHEKTSVVD LIEEIYVSLR QADEVALNDL 150
FNELKDARAK GATNKACEEI ISRIDGFESH VVPIIADIDA GFGNEHATYL 200
LAKEMIKAGA CCLQIENQVS DAKQCGHQDG KVTVPREDFI EKLRACRLAF 250
EELGVDDGVI VARTDSLGAS LTQKIPVSQQ AGDFASSYIK WLKTEPITDA 300
NPLSEGELAI WQSGNFARPI RMPNGLFSFR EGTGRARVIE DCIASLKDGD 350
ADLIWIETDT PNVDEIASMV AEIRKQVPDA KLVYNNSPSF NWTLNLRKQV 400
RAQWISEGKI AEADYPDGTA LMSAQYDTSE LGREADDRLR QFQVDISARA 450
GVFHNLITLP TFHLTAKSTD ELSHGYFGED RMLAYVATVQ REEIRRSISA 500
VRHQHEVGSD LGDTFKEMVS GDRALKAGGA HNTMNQFAAE 540
Length:540
Mass (Da):59,898
Last modified:June 1, 1998 - v1
Checksum:iF289F07879534A7B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB004651 Genomic DNA. Translation: BAA23678.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB004651 Genomic DNA. Translation: BAA23678.1 .

3D structure databases

ProteinModelPortali O50078.
ModBasei Search...

Proteomic databases

PRIDEi O50078.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00719 .
BioCyci RETL1328306-WGS:GSTH-5154-MONOMER.

Family and domain databases

Gene3Di 3.20.20.60. 3 hits.
InterProi IPR006254. Isocitrate_lyase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view ]
PANTHERi PTHR21631:SF3. PTHR21631:SF3. 1 hit.
Pfami PF00463. ICL. 3 hits.
[Graphical view ]
PIRSFi PIRSF001362. Isocit_lyase. 1 hit.
SUPFAMi SSF51621. SSF51621. 2 hits.
ProtoNeti Search...

Publicationsi

  1. "Characterization, gene cloning and expression of isocitrate lyase involved in the assimilation of one-carbon compounds in Hyphomicrobium methylovorum GM2."
    Tanaka Y., Yoshida T., Watanabe K., Izumi Y., Mitsunaga T.
    Eur. J. Biochem. 249:820-825(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-20, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, PATHWAY, SUBUNIT.
    Strain: GM2.

Entry informationi

Entry nameiACEA_HYPME
AccessioniPrimary (citable) accession number: O50078
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: June 1, 1998
Last modified: July 9, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi