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Protein

50S ribosomal protein L4, chloroplastic

Gene

RPL4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

This protein binds directly and specifically to 23S rRNA (By similarity). May play a role in plastid transcriptional regulation.By similarity

GO - Molecular functioni

  • poly(U) RNA binding Source: TAIR
  • rRNA binding Source: UniProtKB-KW
  • structural constituent of ribosome Source: TAIR

GO - Biological processi

  • translation Source: TAIR

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L4, chloroplastic
Alternative name(s):
CL4
R-protein L4
Gene namesi
Name:RPL4
Ordered Locus Names:At1g07320
ORF Names:F22G5.34, F22G5_28
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi

Organism-specific databases

AraportiAT1G07320
TAIRilocus:2025107 AT1G07320

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 49ChloroplastCuratedAdd BLAST49
ChainiPRO_000003053850 – 28250S ribosomal protein L4, chloroplasticAdd BLAST233

Proteomic databases

PaxDbiO50061
PRIDEiO50061

PTM databases

iPTMnetiO50061

Expressioni

Gene expression databases

ExpressionAtlasiO50061 differential
GenevisibleiO50061 AT

Interactioni

Subunit structurei

Part of the 50S ribosomal subunit.By similarity

Protein-protein interaction databases

BioGridi22484, 4 interactors
STRINGi3702.AT1G07320.1

Structurei

3D structure databases

ProteinModelPortaliO50061
SMRiO50061
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi267 – 282Asp/Glu-rich (acidic)Add BLAST16

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1624 Eukaryota
COG0088 LUCA
HOGENOMiHOG000248767
InParanoidiO50061
KOiK02926
OMAiVMVRQNA
OrthoDBiEOG09360NQL
PhylomeDBiO50061

Family and domain databases

Gene3Di3.40.1370.10, 1 hit
HAMAPiMF_01328_B Ribosomal_L4_B, 1 hit
InterProiView protein in InterPro
IPR002136 Ribosomal_L4/L1e
IPR023574 Ribosomal_L4_dom_sf
IPR013005 Ribosomal_uL4/L1e
PANTHERiPTHR10746 PTHR10746, 1 hit
PfamiView protein in Pfam
PF00573 Ribosomal_L4, 1 hit
SUPFAMiSSF52166 SSF52166, 1 hit
TIGRFAMsiTIGR03953 rplD_bact, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O50061-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSATAPNS LSFFSSSLFL SSSHQIPKTY ISVSKLGSGR VSKPLSVSSQ
60 70 80 90 100
LATLPILSFE GEKVGETYLD LKAAPEDTAR AVVHRAIVTD LNNKRRGTAS
110 120 130 140 150
TLTRGEVRGG GIKPYSQKKT GHARRGSQRT PLRPGGGVVF GPRPKDWSIK
160 170 180 190 200
INRKEKKLAI STALSSAASA EGGAIVVEEF GEKFEKPKTK DFLAAMQRWG
210 220 230 240 250
LDPKEKAMFL MIDVDENVAK SSRNIGTLRM LTPRTLNLFD ILNADKLVLT
260 270 280
PAAVEFLNAR YGVDAVEEED DDEDETEGSE EA
Length:282
Mass (Da):30,559
Last modified:June 20, 2002 - v2
Checksum:i1479CC1CEA75F29C
GO

Sequence cautioni

The sequence AAF79563 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti192F → L in CAA74894 (PubMed:9461586).Curated1
Sequence conflicti192F → L in CAA74895 (PubMed:9461586).Curated1
Sequence conflicti207A → P in AAM61637 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y14565 mRNA Translation: CAA74894.1
Y14566 Genomic DNA Translation: CAA74895.1
AC022464 Genomic DNA Translation: AAF79563.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE28106.1
AY063889 mRNA Translation: AAL36245.1
AY117317 mRNA Translation: AAM51392.1
AY085081 mRNA Translation: AAM61637.1
PIRiD86208
RefSeqiNP_001030985.1, NM_001035908.2
NP_001030986.1, NM_001035909.1
NP_563786.1, NM_100606.4 [O50061-1]
UniGeneiAt.10172
At.68122

Genome annotation databases

EnsemblPlantsiAT1G07320.1; AT1G07320.1; AT1G07320 [O50061-1]
AT1G07320.2; AT1G07320.2; AT1G07320
GeneIDi837243
GrameneiAT1G07320.1; AT1G07320.1; AT1G07320 [O50061-1]
AT1G07320.2; AT1G07320.2; AT1G07320
KEGGiath:AT1G07320

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRK4_ARATH
AccessioniPrimary (citable) accession number: O50061
Secondary accession number(s): Q8LF27, Q8VZS1, Q9LNV1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 20, 2002
Last modified: May 23, 2018
This is version 123 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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