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Reviewed, UniProtKB/Swiss-Prot O50039 (OTC_ARATH)

Last modified November 3, 2009. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Ornithine carbamoyltransferase, chloroplastic
    EC=2.1.3.3
Alternative name(s):
    Ornithine transcarbamylase
      Short name=OTCase
Gene names
Name: OTC
Ordered Locus Names: At1g75330
ORF Names: F1B16.13
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length375 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.

Subcellular location

Plastid › chloroplast.

Sequence similarities

Belongs to the ATCase/OTCase family.

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Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentchloroplast stroma

Inferred from direct assay. Source: TAIR

ornithine carbamoyltransferase complex

Inferred from electronic annotation. Source: InterPro

   Molecular functionamino acid binding

Inferred from electronic annotation. Source: InterPro

ornithine carbamoyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Chloroplast
Chain? – 375Ornithine carbamoyltransferase, chloroplasticPRO_0000020340

Regions

Region123 – 1275Ornithine and carbamoyl phosphate binding By similarity
Region201 – 2044Ornithine and carbamoyl phosphate binding By similarity
Region293 – 2975Ornithine binding By similarity
Region332 – 3354Ornithine binding By similarity

Sites

Active site3331 By similarity
Binding site1741Carbamoyl phosphate By similarity
Binding site1741Ornithine By similarity
Binding site2011Carbamoyl phosphate By similarity
Binding site2321Ornithine By similarity
Binding site3611Carbamoyl phosphate By similarity
Binding site3611Ornithine By similarity

Experimental info

Sequence conflict1581R → P in CAA04115. Ref.1
Sequence conflict1581R → P in CAA05510. Ref.1
Sequence conflict2141E → K in CAA04115. Ref.1
Sequence conflict2141E → K in CAA05510. Ref.1
Sequence conflict219 – 2224VEGT → FERK in CAA04115. Ref.1
Sequence conflict219 – 2224VEGT → FERK in CAA05510. Ref.1
Sequence conflict3661N → Y in CAA04115. Ref.1
Sequence conflict3661N → Y in CAA05510. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
O50039-1 [UniParc].

Last modified July 11, 2001. Version 2.
Checksum: 9DA2A0B512FCA323

FASTA37541,002
        10         20         30         40         50         60 
MAAAMASHVS TARSPALSFS SSSSSFFPGT TLRRFSAVSL PSPALPRLRV SCQASSVTSP 

        70         80         90        100        110        120 
SSPSDVKGKS DLKDFLAIDD FDTATIKTIL DKASEVKALL KSGERNYLPF KGKSMSMIFA 

       130        140        150        160        170        180 
KPSMRTRVSF ETGFFLLGGH ALYLGPNDIQ MGKREETRDV ARVLSRYNDI IMARVFAHQD 

       190        200        210        220        230        240 
ILDLANYSSV PVVNGLTDHN HPCQIMADAL TMIEHIGQVE GTKVVYVGDG NNMVHSWLEL 

       250        260        270        280        290        300 
ASVIPFHFVC ACPKGYEPDK ERVSKAKQAG LSKIEITNDP KEAVIGADVV YSDVWASMGQ 

       310        320        330        340        350        360 
KDEAEARRKA FQGFQVDEAL MKLAGQKAYF MHCLPAERGV EVTNGVVEAP YSIVFPQAEN 

       370 
RMHAQNAIML HLLGF

« Hide

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References

« Hide 'large scale' references
[1]"OTC and AUL1, two convergent and overlapping genes in the nuclear genome of Arabidopsis thaliana."
Quesada V., Ponce M.R., Micol J.L.
FEBS Lett. 461:101-106(1999) [PubMed: 10561504] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed: 11130712] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AJ000476 mRNA. Translation: CAA04115.1.
AJ002524 Genomic DNA. Translation: CAA05510.1.
AC023754 Genomic DNA. Translation: AAG13075.1.
AF370270 mRNA. Translation: AAK44085.1.
AY063030 mRNA. Translation: AAL34204.1.
IPIIPI00533595.
PIRG96783.
T51617.
RefSeqNP_177667.1.
UniGeneAt.545

3D structure databases

HSSPHSSP built from PDB template 1A1S based on UniProtKB Q51742.
ModBaseSearch...

Protein-protein interaction databases

STRINGO50039.

Proteomic databases

PRIDEO50039.
ProMEXO50039.

Genome annotation databases

GeneID843869.
GenomeReviewsGene locus AT1G75330 in contig CT485782_GR.
KEGGath:AT1G75330.
NMPDRfig|3702.1.peg.7052.

Organism-specific databases

TAIRAt1g75330.

Phylogenomic databases

OMAMTFEEHR.

Enzyme and pathway databases

BRENDA2.1.3.3. 302.

Gene expression databases

ArrayExpressO50039.
GenevestigatorO50039.
GermOnlineAT1G75330. Arabidopsis thaliana.

Family and domain databases

InterProIPR006132. Asp/Orn_carbamoyltranf_P_bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn_bd.
IPR002292. Orn_carbamltrans.
[Graphical view]
PfamPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSPR00100. AOTCASE.
PR00102. OTCASE.
TIGRFAMsTIGR00658. orni_carb_tr. 1 hit.
PROSITEPS00097. CARBAMOYLTRANSFERASE. False negative.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameOTC_ARATH
AccessionPrimary (citable) accession number: O50039
Secondary accession number(s): Q9FWS2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 11, 2001
Last modified: November 3, 2009
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents