O50017 (PARP2_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Poly [ADP-ribose] polymerase 2
Short name=PARP-2
EC=2.4.2.30

Alternative name(s):
NAD(+) ADP-ribosyltransferase 2
Short name=ADPRT-2
Poly[ADP-ribose] synthase 2

Gene names

Name:	PARP2
Synonyms:	PARP

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	653 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks By similarity.
Catalytic activity	NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.
Subcellular location	Nucleus Potential.
Sequence similarities	Contains 1 PARP alpha-helical domain. Contains 1 PARP catalytic domain. Contains 2 SAP domains.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Repeat
Ligand	DNA-binding NAD
Molecular function	Glycosyltransferase Transferase
PTM	ADP-ribosylation
Gene Ontology (GO)
Biological process	protein ADP-ribosylation Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ ADP-ribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 653	653	Poly [ADP-ribose] polymerase 2		PRO_0000260499
Regions
	Domain	2 – 36	35	SAP 1
	Domain	91 – 125	35	SAP 2
	Domain	301 – 419	119	PARP alpha-helical
	Domain	427 – 653	227	PARP catalytic
	Motif	69 – 72	4	Nuclear localization signal Potential

Sequences

	Sequence		Length	Mass (Da)
	O50017 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 5FD01923C4ABCD1D	FASTA	653	72,995
	10 20 30 40 50 60 MSARLRVADV RAELQRRGLD VSGTKPALVR RLDAAICEAE KAVVAAAPTS VANGYDVAVD 70 80 90 100 110 120 GKRNCGNNKR KRSGDGGEEG NGDTCTDVTK LEGMSYRELQ GLAKARGVAA NGGKKDVIQR 130 140 150 160 170 180 LLSATAGPAA VADGGPLGAK EVIKGGDEEV EVKKEKMVTA TKKGAAVLDQ HIPDHIKVNY 190 200 210 220 230 240 HVLQVGDEIY DATLNQTNVG DNNNKFYIIQ VLESDAGGSF MVYNRWGRVG VRGQDKLHGP 250 260 270 280 290 300 SPTRDQAIYE FEGKFHNKTN NHWSDRKNFK CYAKKYTWLE MDYGETEKEI EKGSITDQIK 310 320 330 340 350 360 ETKLETRIAQ FISLICNISM MKQRMVEIGY NAEKLPLGKL RKATILKGYH VLKRISDVIS 370 380 390 400 410 420 KADRRHLEQL TGEFYTVIPH DFGFRKMREF IIDTPQKLKA KLEMVEALGE IEIATKLLED 430 440 450 460 470 480 DSSDQDDPLY ARYKQLHCDF TPLEADSDEY SMIKSYLRNT HGKTHSGYTV DIVQIFKVSR 490 500 510 520 530 540 HGETERFQKF ASTRNRMLLW HGSRLSNWAG ILSQGLRIAP PEAPVTGYMF GKGVYFADMF 550 560 570 580 590 600 SKSANYCYAS EACRSGVLLL CEVALGDMNE LLNADYDANN LPKGKLRSKG VGQTAPNMVE 610 620 630 640 650 SKVADDGVVV PLGEPKQEPS KRGGLLYNEY IVYNVDQIRM RYVLHVNFNF KRR

References

[1]	"Higher plants possess two structurally different poly(ADP-ribose) polymerases." Babiychuk E., Cottrill P.B., Storozhenko S., Fuangthong M., Chen Y., O'Farrell M.K., Van Montagu M., Inze D., Kushnir S. Plant J. 15:635-645(1998) [PubMed: 9778846] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ222588 mRNA. Translation: CAA10888.1.
PIR	T03656.
3D structure databases
HSSP	HSSP built from PDB template 1GS0 based on UniProtKB O88554.
ProteinModelPortal	O50017.
SMR	O50017. Positions 3-38.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	O50017.
MaizeGDB	403201.
Phylogenomic databases
GeneTree	EPGT00070000029473.
Family and domain databases
InterPro	IPR012317. Poly(ADP-ribose)pol_cat_dom. IPR004102. Poly(ADP-ribose)pol_reg_dom. IPR003034. SAP_DNA-bd. IPR008893. WGR_domain. [Graphical view]
Gene3D	G3DSA:1.10.720.30. G3DSA:1.10.720.30. 1 hit. G3DSA:2.20.140.10. G3DSA:2.20.140.10. 1 hit. G3DSA:1.20.142.10. PARP_reg. 1 hit.
Pfam	PF00644. PARP. 1 hit. PF02877. PARP_reg. 1 hit. PF02037. SAP. 1 hit. PF05406. WGR. 1 hit. [Graphical view]
SMART	SM00513. SAP. 2 hits. SM00773. WGR. 1 hit. [Graphical view]
SUPFAM	SSF47587. PARP_reg. 1 hit. SSF142921. SSF142921. 1 hit.
PROSITE	PS51060. PARP_ALPHA_HD. 1 hit. PS51059. PARP_CATALYTIC. 1 hit. PS50800. SAP. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PARP2_MAIZE

Accession

Primary (citable) accession number: O50017

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	June 1, 1998
Last modified:	November 16, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program