Reviewed, UniProtKB/Swiss-Prot O50008 (METE_ARATH)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Vitamin-B12-independent methionine synthase isozyme
    Cobalamin-independent methionine synthase isozyme

Gene names

Name:	CIMS
Ordered Locus Names:	At5g17920
ORF Names:	MPI7.9

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	765 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine.
Cofactor	Zinc By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW response to cadmium ion Inferred from expression pattern. Source: TAIR response to salt stress Inferred from expression pattern. Source: TAIR
Cellular component	apoplast Inferred from direct assay. Source: TAIR chloroplast stroma Inferred from direct assay. Source: TAIR cytosol Inferred from direct assay. Source: TAIR peroxisome Inferred from direct assay. Source: TAIR plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC copper ion binding Inferred from direct assay. Source: TAIR methionine synthase activity Inferred from direct assay. Source: TAIR zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 765

765

5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase

PRO_0000098696

Sites

Metal binding

647

1

Zinc By similarity

Metal binding

649

1

Zinc By similarity

Metal binding

733

1

Zinc By similarity

Secondary structure

1

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765

Helix Strand Turn

Details...

Helix

16 – 26

11

Helix

31 – 51

21

Beta strand

58 – 60

3

Helix

67 – 74

8

Helix

80 – 82

3

Beta strand

86 – 88

3

Helix

91 – 99

9

Beta strand

102 – 104

3

Beta strand

109 – 111

3

Beta strand

118 – 120

3

Helix

136 – 146

11

Beta strand

152 – 156

5

Helix

158 – 163

6

Helix

177 – 180

4

Helix

181 – 197

17

Beta strand

202 – 206

5

Helix

208 – 211

4

Helix

216 – 228

13

Turn

230 – 233

4

Beta strand

235 – 241

7

Helix

249 – 255

7

Beta strand

263 – 270

8

Helix

274 – 280

7

Beta strand

287 – 293

7

Beta strand

295 – 297

3

Helix

303 – 316

14

Beta strand

322 – 328

7

Helix

330 – 332

3

Helix

337 – 339

3

Helix

345 – 348

4

Helix

354 – 368

15

Helix

374 – 389

16

Turn

391 – 393

3

Helix

397 – 400

4

Turn

401 – 405

5

Helix

416 – 418

3

Helix

420 – 426

7

Helix

463 – 479

17

Beta strand

484 – 486

3

Helix

498 – 501

4

Beta strand

515 – 518

4

Beta strand

521 – 523

3

Helix

541 – 546

6

Beta strand

554 – 559

6

Helix

561 – 566

6

Beta strand

568 – 570

3

Helix

576 – 596

21

Beta strand

601 – 605

5

Helix

617 – 619

3

Helix

620 – 635

16

Beta strand

640 – 647

8

Turn

654 – 656

3

Helix

657 – 661

5

Beta strand

666 – 669

4

Beta strand

672 – 675

4

Helix

679 – 683

5

Turn

684 – 687

4

Beta strand

691 – 693

3

Beta strand

696 – 698

3

Beta strand

702 – 704

3

Helix

708 – 720

13

Beta strand

735 – 738

4

Helix

740 – 759

20

Sequences

	Sequence		Length	Mass (Da)
	O50008-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 2EA01908B5951154	FASTA	765	84,357
	10 20 30 40 50 60 MASHIVGYPR MGPKRELKFA LESFWDGKST AEDLQKVSAD LRSSIWKQMS AAGTKFIPSN 70 80 90 100 110 120 TFAHYDQVLD TTAMLGAVPP RYGYTGGEIG LDVYFSMARG NASVPAMEMT KWFDTNYHYI 130 140 150 160 170 180 VPELGPEVNF SYASHKAVNE YKEAKALGVD TVPVLVGPVS YLLLSKAAKG VDKSFELLSL 190 200 210 220 230 240 LPKILPIYKE VITELKAAGA TWIQLDEPVL VMDLEGQKLQ AFTGAYAELE STLSGLNVLV 250 260 270 280 290 300 ETYFADIPAE AYKTLTSLKG VTAFGFDLVR GTKTLDLVKA GFPEGKYLFA GVVDGRNIWA 310 320 330 340 350 360 NDFAASLSTL QALEGIVGKD KLVVSTSCSL LHTAVDLINE TKLDDEIKSW LAFAAQKVVE 370 380 390 400 410 420 VNALAKALAG QKDEALFSAN AAALASRRSS PRVTNEGVQK AAAALKGSDH RRATNVSARL 430 440 450 460 470 480 DAQQKKLNLP ILPTTTIGSF PQTVELRRVR REYKAKKVSE EDYVKAIKEE IKKVVDLQEE 490 500 510 520 530 540 LDIDVLVHGE PERNDMVEYF GEQLSGFAFT ANGWVQSYGS RCVKPPVIYG DVSRPKAMTV 550 560 570 580 590 600 FWSAMAQSMT SRPMKGMLTG PVTILNWSFV RNDQPRHETC YQIALAIKDE VEDLEKGGIG 610 620 630 640 650 660 VIQIDEAALR EGLPLRKSEH AFYLDWAVHS FRITNCGVQD STQIHTHMCY SHFNDIIHSI 670 680 690 700 710 720 IDMDADVITI ENSRSDEKLL SVFREGVKYG AGIGPGVYDI HSPRIPSSEE IADRVNKMLA 730 740 750 760 VLEQNILWVN PDCGLKTRKY TEVKPALKNM VDAAKLIRSQ LASAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The specific features of methionine biosynthesis and metabolism in plants." Ravanel S., Gakiere B., Job D., Douce R. Proc. Natl. Acad. Sci. U.S.A. 95:7805-7812(1998) [PubMed: 9636232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Strain: cv. Columbia.
[2]	"Characterization of the cDNA and gene for a cytosolic cobalamin-independent methionine synthase in Arabidopsis thaliana." Gakiere B., Job D., Douce R., Ravanel S. Plant Gene Register PGR99-115 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones." Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S. DNA Res. 5:131-145(1998) [PubMed: 9679202] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U97200 mRNA. Translation: AAC50037.1.
AB011480 Genomic DNA. Translation: BAB11226.1.
AF370522 mRNA. Translation: AAK43899.1.
AY048201 mRNA. Translation: AAK82464.1.
AY056098 mRNA. Translation: AAL06986.1.
AY057499 mRNA. Translation: AAL09740.1.
AY069876 mRNA. Translation: AAL47432.1.
AY070771 mRNA. Translation: AAL50108.1.
AY091692 mRNA. Translation: AAM10291.1.

IPI

IPI00522440.

RefSeq

NP_001078599.1.
NP_197294.1.

UniGene

At.22340
At.71028

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1U1H	X-ray	2.55	A	1-765	[»]
1U1J	X-ray	2.40	A	1-765	[»]
1U1U	X-ray	2.95	A	1-765	[»]
1U22	X-ray	2.65	A	1-765	[»]

ModBase

Search...

2-D gel databases

SWISS-2DPAGE

O50008.

Proteomic databases

PRIDE

O50008.

ProMEX

O50008.

Genome annotation databases

GeneID

831660.

GenomeReviews

Gene locus AT5G17920 in contig BA000015_GR.

KEGG

ath:AT5G17920.

NMPDR

fig|3702.1.peg.23963.

Organism-specific databases

TAIR

At5g17920.

Phylogenomic databases

OMA

TYLWLSK.

Enzyme and pathway databases

BRENDA

2.1.1.14. 302.

Gene expression databases

Genevestigator

O50008.

GermOnline

AT5G17920. Arabidopsis thaliana.

Family and domain databases

InterPro

IPR013215. Cbl-indep_Met_Synth_N.
IPR002629. Methionine_synth.
IPR006276. MeTrfase_B12_ind.
[Graphical view]

Pfam

PF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]

PIRSF

PIRSF000382. MeTrfase_B12_ind. 1 hit.

ProDom

PD004692. Methionine_synth. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR01371. met_syn_B12ind. 1 hit.

ProtoNet

Search...

Entry information

Entry name

METE_ARATH

Accession

Primary (citable) accession number: O50008

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	June 1, 1998
Last modified:	November 3, 2009
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)