O49901 (ZDS_NARPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zeta-carotene desaturase, chloroplastic/chromoplastic
EC=1.3.5.6

Alternative name(s):
9,9'-di-cis-zeta-carotene desaturase
Carotene 7,8-desaturase

Gene names

Name:

ZDS1

Organism

Narcissus pseudonarcissus (Daffodil)

Taxonomic identifier

39639 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › Asparagales › Amaryllidaceae › Amaryllidoideae › Narcissus

Protein attributes

Sequence length	574 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the conversion of zeta-carotene to lycopene via the intermediary of neurosporene. It carries out two consecutive desaturations (introduction of double bonds) at positions C-7 and C-7'.
Catalytic activity	9,9'-di-cis-zeta-carotene + 2 quinone = 7,9,7',9'-tetra-cis-lycopene + 2 quinol.
Cofactor	NAD, NADP, or FAD Probable.
Pathway	Carotenoid biosynthesis; lycopene biosynthesis.
Subcellular location	Plastid › chloroplast. Plastid › chromoplast.
Sequence similarities	Belongs to the zeta carotene desaturase family.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis
Cellular component	Chloroplast Chromoplast Plastid
Domain	Transit peptide
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	9,9'-di-cis-zeta-carotene desaturation to 7,9,7',9'-tetra-cis-lycopene Inferred from electronic annotation. Source: EC carotenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell chromoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	7,9,9'-tricis-neurosporene:quinone oxidoreductase activity Inferred from electronic annotation. Source: EC 9,9'-dicis-carotene:quinone oxidoreductase activity Inferred from electronic annotation. Source: EC carotene 7,8-desaturase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast and chromoplast Potential
	Chain	? – 574		Zeta-carotene desaturase, chloroplastic/chromoplastic		PRO_0000041609

Sequences

	Sequence		Length	Mass (Da)
	O49901 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 67D2CBD4FF942FF7	FASTA	574	63,582
	10 20 30 40 50 60 MASSTCLIHS SSFGVGGKKV KMNTMIRSKL FSIRSALDTK VSDMSVNAPK GLFPPEPEHY 70 80 90 100 110 120 RGPKLKVAII GAGLAGMSTA VELLDQGHEV DIYESRQFIG GKVGSFVDKR GNHIEMGLHV 130 140 150 160 170 180 FFGCYNNLFR LMKKVGADEN LLVKDHTHTF VNRGGEIGEL DFRLPMGAPL HGIRAFLTTN 190 200 210 220 230 240 QLKPYDKARN AVALALSPVV RALIDPNGAM QDIRNLDNIS FSDWFLSKGG TRMSIQRMWD 250 260 270 280 290 300 PVAYALGFID CDNISARCML TIFSLFATKT EASLLRMLKG SPDVYLSGPI RKYITDKGGR 310 320 330 340 350 360 FHLRWGCREI LYDELSNGDT YITGIAMSKA TNKKLVKADV YVAACDVPGI KRLIPSEWRE 370 380 390 400 410 420 WDLFDNIYKL VGVPVVTVQL RYNGWVTEMQ DLEKSRQLRA AVGLDNLLYT PDADFSCFSD 430 440 450 460 470 480 LALSSPEDYY IEGQGSLIQA VLTPGDPYMP LPNDAIIERV RKQVLDLFPS SQGLEVLWSS 490 500 510 520 530 540 VVKIGQSLYR EGPGKDPFRP DQKTPVKNFF LAGSYTKQDY IDSMEGATLS GRQAAAYICS 550 560 570 AGEDLAALRK KIAADHPEQL INKDSNVSDE LSLV

References

[1]	"A cDNA encoding for beta carotene desaturase from Narcissus pseudonarcissus L." Al-Babili S., Oelschlegel J., Beyer P. Plant Gene Register PGR98-103 Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ224683 mRNA. Translation: CAA12062.1.
3D structure databases
ProteinModelPortal	O49901.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00803.
Family and domain databases
InterPro	IPR002937. Amino_oxidase. IPR014103. Zeta_caro_desat. [Graphical view]
Pfam	PF01593. Amino_oxidase. 1 hit. [Graphical view]
TIGRFAMs	TIGR02732. zeta_caro_desat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ZDS_NARPS

Accession

Primary (citable) accession number: O49901

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 2, 2001
Last sequence update:	June 1, 1998
Last modified:	October 3, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program