Reviewed, UniProtKB/Swiss-Prot O49886 (CYPH_LUPLU)

Names and origin

Protein names	Recommended name:     Peptidyl-prolyl cis-trans isomerase       Short name=PPIase       Short name=Rotamase     EC=5.2.1.8 Alternative name(s):     Cyclophilin     Cyclosporin A-binding protein
Organism	Lupinus luteus (European yellow lupin)
Taxonomic identifier	3873 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Genisteae › Lupinus

Protein attributes

Sequence length	172 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Catalytic activity	Peptidylproline (omega=180) = peptidylproline (omega=0).
Enzyme regulation	Binds cyclosporin A (CsA). CsA mediates some of its effects via an inhibitory action on PPIase.
Subcellular location	Cytoplasm.
Tissue specificity	Expressed in meristematic tissues, with higher levels in nodules. Ref.1
Sequence similarities	Belongs to the cyclophilin-type PPIase family. Contains 1 PPIase cyclophilin-type domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Cyclosporin
Molecular function	Isomerase Rotamase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	peptide binding Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-prolyl cis-trans isomerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 172

172

Peptidyl-prolyl cis-trans isomerase

PRO_0000064144

Regions

Domain

7 – 170

164

PPIase cyclophilin-type

Secondary structure

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172

Helix Strand Turn

Details...

Beta strand

5 – 12

8

Beta strand

15 – 24

10

Turn

26 – 28

3

Helix

30 – 41

12

Turn

42 – 44

3

Beta strand

50 – 53

4

Beta strand

62 – 64

3

Turn

65 – 67

3

Beta strand

68 – 71

4

Beta strand

74 – 80

7

Beta strand

104 – 107

4

Beta strand

119 – 124

6

Helix

127 – 129

3

Beta strand

135 – 141

7

Helix

143 – 151

9

Beta strand

155 – 157

3

Beta strand

163 – 170

8

Sequences

	Sequence		Length	Mass (Da)
	O49886-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: DD8351276CBEEF1F	FASTA	172	18,287
	10 20 30 40 50 60 MSNPKVFFDM AIAGNPAGRI VMELYADTTP RTAENFRALC TGEKGVGRSG KPLHYKGSTF 70 80 90 100 110 120 HRVIPNFMCQ GGDFTAGNGT GAESIYGAKF ADENFIKRHT GPGILSMANA GAGTNGSQFF 130 140 150 160 170 ICTEKTEWLD GKHVVFGKVI EGMNVVRDIE KVGSGSGKTS RPVTIADCGQ LS

References

[1]	"Yellow lupine cyclophilin transcripts are highly accumulated in the nodule meristem zone." Nuc K., Nuc P., Slomski R. Mol. Plant Microbe Interact. 14:1384-1394(2001) [PubMed: 11768533] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY. Strain: cv. Ventus. Tissue: Seedling.
[2]	"Theoretical model of CYPH_LUPLU." Siddharhta K. Submitted (AUG-2002) to the PDB data bank Cited for: 3D-STRUCTURE MODELING.

Cross-references

Sequence databases

EMBLGenBankDDBJ

Y16088 mRNA. Translation: CAA76054.1.
AF178458 Genomic DNA. Translation: AAF00471.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1MD5	model	-	A	1-172	[»]

SMR

O49886. Positions 1-171.

ModBase

Search...

Enzyme and pathway databases

BRENDA

5.2.1.8. 261.

Family and domain databases

InterPro

IPR002130. PPIase_cyclophilin.
[Graphical view]

Gene3D

G3DSA:2.40.100.10. PPIase_cyclophilin. 1 hit.

Pfam

PF00160. Pro_isomerase. 1 hit.
[Graphical view]

PRINTS

PR00153. CSAPPISMRASE.

PROSITE

PS00170. CSA_PPIASE_1. 1 hit.
PS50072. CSA_PPIASE_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CYPH_LUPLU

Accession

Primary (citable) accession number: O49886

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)