O49840 (APK2B_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein kinase 2B, chloroplastic
EC=2.7.11.1

Gene names

Name:	APK2B
Ordered Locus Names:	At2g02800
ORF Names:	T20F6.6

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Plastid › chloroplast Potential.
Tissue specificity	Strongly expressed in leaves, moderately in flowers, and barely in roots. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay. Source: TAIR plasma membrane Inferred from direct assay Ref.6Ref.7. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 69	69	Chloroplast Potential
	Chain	70 – 426	357	Protein kinase 2B, chloroplastic		PRO_0000389477
Regions
	Domain	83 – 366	284	Protein kinase
	Nucleotide binding	89 – 97	9	ATP By similarity
Sites
	Active site	216	1	Proton acceptor By similarity
	Binding site	121	1	ATP By similarity
Amino acid modifications
	Modified residue	87	1	Phosphoserine Ref.6
	Modified residue	114	1	Phosphoserine Ref.6
Experimental info
	Sequence conflict	295	1	K → R in BAC42058. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	O49840 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: C73C695C98501EBA	FASTA	426	46,289
	10 20 30 40 50 60 MGNCLDSSAK VDSSSHSPHA NSASLSSRVS SKTSRSTVPS SLSINSYSSV ESLPTPRTEG 70 80 90 100 110 120 EILSSPNLKA FTFNELKNAT RNFRPDSLLG EGGFGYVFKG WIDGTTLTAS KPGSGIVVAV 130 140 150 160 170 180 KKLKTEGYQG HKEWLTEVNY LGQLSHPNLV KLVGYCVEGE NRLLVYEFMP KGSLENHLFR 190 200 210 220 230 240 RGAQPLTWAI RMKVAIGAAK GLTFLHDAKS QVIYRDFKAA NILLDAEFNS KLSDFGLAKA 250 260 270 280 290 300 GPTGDKTHVS TQVMGTHGYA APEYVATGRL TAKSDVYSFG VVLLELLSGR RAVDKSKVGM 310 320 330 340 350 360 EQSLVDWATP YLGDKRKLFR IMDTRLGGQY PQKGAYTAAS LALQCLNPDA KLRPKMSEVL 370 380 390 400 410 420 AKLDQLESTK PGTGVGNRQA QIDSPRGSNG SIVQKSPRRY SYDRPLLHIT PGASPLPTHN HSPRVR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A serine/threonine protein kinase gene isolated by an in vivo binding procedure using the Arabidopsis floral homeotic gene product, AGAMOUS." Ito T., Takahashi N., Shimura Y., Okada K. Plant Cell Physiol. 38:248-258(1997) [PubMed: 9150601] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: cv. Landsberg erecta.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed: 11910074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Quantitative phosphoproteomics of early elicitor signaling in Arabidopsis." Benschop J.J., Mohammed S., O'Flaherty M., Heck A.J.R., Slijper M., Menke F.L.H. Mol. Cell. Proteomics 6:1198-1214(2007) [PubMed: 17317660] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87 AND SER-114. Strain: cv. Columbia.
[7]	"A high content in lipid-modified peripheral proteins and integral receptor kinases features in the arabidopsis plasma membrane proteome." Marmagne A., Ferro M., Meinnel T., Bruley C., Kuhn L., Garin J., Barbier-Brygoo H., Ephritikhine G. Mol. Cell. Proteomics 6:1980-1996(2007) [PubMed: 17644812] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Web resources

PlantP kinase Classification PPC

Cross-references

Sequence databases
EMBL GenBank DDBJ	D88207 mRNA. Translation: BAA24695.1. AC002521 Genomic DNA. Translation: AAC05342.1. CP002685 Genomic DNA. Translation: AEC05625.1. CP002685 Genomic DNA. Translation: AEC05626.1. AK117389 mRNA. Translation: BAC42058.1. AF428432 mRNA. Translation: AAL16201.1. AY125559 mRNA. Translation: AAM78069.1.
IPI	IPI00517259.
PIR	T00848.
RefSeq	NP_178383.1. NM_126335.5. NP_973403.1. NM_201674.2.
UniGene	At.21329. At.52607.
3D structure databases
ProteinModelPortal	O49840.
SMR	O49840. Positions 72-369.
ModBase	Search...
Protein-protein interaction databases
IntAct	O49840. 2 interactions.
STRING	O49840.
Proteomic databases
PRIDE	O49840.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G02800.1; AT2G02800.1; AT2G02800. AT2G02800.2; AT2G02800.2; AT2G02800.
GeneID	814810.
GenomeReviews	Gene locus AT2G02800 in contig CT485783_GR.
KEGG	ath:AT2G02800.
NMPDR	fig|3702.1.peg.7876.
Organism-specific databases
GeneFarm	1719. 129.
TAIR	At2g02800.
Phylogenomic databases
eggNOG	KOG1187.
GeneTree	EPGT00050000000099.
HOGENOM	HBG755340.
InParanoid	O49840.
OMA	GWIDEHT.
PhylomeDB	O49840.
ProtClustDB	CLSN2682936.
Gene expression databases
ArrayExpress	O49840.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001245. Ser-Thr/Tyr_kinase. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

APK2B_ARATH

Accession

Primary (citable) accession number: O49840
Secondary accession number(s): Q8GYU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 24, 2009
Last sequence update:	June 1, 1998
Last modified:	October 19, 2011
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program