O49839 (APK2A_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein kinase 2A, chloroplastic
EC=2.7.11.1

Gene names

Name:	APK2A
Ordered Locus Names:	At1g14370
ORF Names:	F14L17.14

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Plastid › chloroplast Potential.
Tissue specificity	Strongly expressed in leaves, moderately in roots, and barely in flowers, mostly in pedicels. Ref.1
Induction	Negatively regulated by AGAMOUS (AG) in floral organ primordia. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 72	72	Chloroplast Potential
	Chain	73 – 426	354	Protein kinase 2A, chloroplastic		PRO_0000389476
Regions
	Domain	86 – 369	284	Protein kinase
	Nucleotide binding	92 – 100	9	ATP By similarity
Sites
	Active site	219	1	Proton acceptor By similarity
	Binding site	124	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O49839 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 51A7670B74C24DD3	FASTA	426	46,296
	10 20 30 40 50 60 MGNCLDSSAK VDNSNHSPHA NSASSGSKVS SKTSRSTGPS GLSTTSYSTD SSFGPLPTLR 70 80 90 100 110 120 TEGEILSSPN LKAFTFNELK NATKNFRQDN LLGEGGFGCV FKGWIDQTSL TASRPGSGIV 130 140 150 160 170 180 VAVKQLKPEG FQGHKEWLTE VNYLGQLSHP NLVLLVGYCA EGENRLLVYE FMPKGSLENH 190 200 210 220 230 240 LFRRGAQPLT WAIRMKVAVG AAKGLTFLHE AKSQVIYRDF KAANILLDAD FNAKLSDFGL 250 260 270 280 290 300 AKAGPTGDNT HVSTKVIGTH GYAAPEYVAT GRLTAKSDVY SFGVVLLELI SGRRAMDNSN 310 320 330 340 350 360 GGNEYSLVDW ATPYLGDKRK LFRIMDTKLG GQYPQKGAFT AANLALQCLN PDAKLRPKMS 370 380 390 400 410 420 EVLVTLEQLE SVAKPGTKHT QMESPRFHHS SVMQKSPVRY SHDRPLLHMT PGASPLPSYT QSPRVR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A serine/threonine protein kinase gene isolated by an in vivo binding procedure using the Arabidopsis floral homeotic gene product, AGAMOUS." Ito T., Takahashi N., Shimura Y., Okada K. Plant Cell Physiol. 38:248-258(1997) [PubMed: 9150601] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], REGULATION BY AGAMOUS, TISSUE SPECIFICITY. Strain: cv. Landsberg erecta.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Web resources

PlantP kinase Classification PPC

Cross-references

Sequence databases
EMBL GenBank DDBJ	D88206 mRNA. Translation: BAA24694.1. AC012188 Genomic DNA. Translation: AAF43937.1. CP002684 Genomic DNA. Translation: AEE29153.1. AF334731 mRNA. Translation: AAG50109.1. AY059894 mRNA. Translation: AAL24376.1.
IPI	IPI00546682.
PIR	T52285.
RefSeq	NP_172889.1. NM_101304.3.
UniGene	At.48188. At.70085.
3D structure databases
ProteinModelPortal	O49839.
SMR	O49839. Positions 76-372.
ModBase	Search...
Protein-protein interaction databases
IntAct	O49839. 5 interactions.
STRING	O49839.
Proteomic databases
PRIDE	O49839.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G14370.1; AT1G14370.1; AT1G14370.
GeneID	837999.
GenomeReviews	Gene locus AT1G14370 in contig CT485782_GR.
KEGG	ath:AT1G14370.
NMPDR	fig|3702.1.peg.1718.
Organism-specific databases
GeneFarm	1718. 129.
TAIR	At1g14370.
Phylogenomic databases
eggNOG	KOG1187.
GeneTree	EPGT00050000000099.
HOGENOM	HBG755340.
InParanoid	O49839.
OMA	ISGRRAM.
PhylomeDB	O49839.
ProtClustDB	CLSN2682936.
Gene expression databases
ArrayExpress	O49839.
Genevestigator	O49839.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001245. Ser-Thr/Tyr_kinase. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
KO	K00924.
Pfam	PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

APK2A_ARATH

Accession

Primary (citable) accession number: O49839

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 24, 2009
Last sequence update:	June 1, 1998
Last modified:	November 16, 2011
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program