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O49809 (MFPA_BRANA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a

Including the following 2 domains:

  1. Enoyl-CoA hydratase/3-2-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase
    EC=4.2.1.17
    EC=5.1.2.3
    EC=5.3.3.8
  2. 3-hydroxyacyl-CoA dehydrogenase
    EC=1.1.1.35
OrganismBrassica napus (Rape)
Taxonomic identifier3708 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Protein attributes

Sequence length725 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O. UniProtKB Q39659

(3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.

(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA. UniProtKB Q39659

(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH. UniProtKB Q39659

Pathway

Lipid metabolism; fatty acid beta-oxidation.

Subcellular location

Glyoxysome By similarity.

Domain

The epimerase and isomerase activities are contained in the N-terminal region while the dehydrogenase activity is in the C-terminal region By similarity. UniProtKB Q39659

Sequence similarities

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.

In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 725725Glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a
PRO_0000109250

Regions

Motif723 – 7253Microbody targeting signal Potential

Sites

Active site1191Nucleophile Potential
Active site1391Proton acceptor Potential

Sequences

Sequence LengthMass (Da)Tools
O49809 [UniParc].

Last modified December 1, 2001. Version 2.
Checksum: 6627C52D433EA976

FASTA72579,023
        10         20         30         40         50         60 
MASRTKGTTT IEVGADGVAV ITLINPPVNS LSFDVLYSLK SNYEEALSRN DVKAIVVTGA 

        70         80         90        100        110        120 
KGKFSGGFDI SGFGEIQKGT MKEPKVGYIS IDILTDLLEA AKKPSVAAID GLALGGGLEL 

       130        140        150        160        170        180 
SMACHARISA PGAQLGLPEL QLGVIPGFGG TQRLPRLVGL TKALEMILTS KPVKAEEGHS 

       190        200        210        220        230        240 
LGLIDAVVPP AELLNAARRW ALDIAERRKP WVSSVLKTDK LPPLGEAREI LKFAKDQTRR 

       250        260        270        280        290        300 
QAPNMKHPLM CLEAVEVGIV SGSRAGLEKE AQVGSEVINL DTTKGLIHVF FSQRGTTKVP 

       310        320        330        340        350        360 
GVTDRGLVPR KINKVAIIGG GLMGSGIATA LILSNYSVIL KEVNEKFLEA GIGRVKANLQ 

       370        380        390        400        410        420 
SRVKKGKMSK EKFEKTMSLL KGSLDYESFR DVDMVIEAVI ENISLKQQIF ADLEKYCPQH 

       430        440        450        460        470        480 
CILASNTSTI DLNKIGERTK SQDRIIGAHF FSPAHVMPLL EIVRTNHTSA QVIVDLLDVG 

       490        500        510        520        530        540 
KKIRKTPVVV GNCTGFAVNR MFFPYTQAAM FLVEHGTDPY LIDKAVSKFG MPMGPFRLCD 

       550        560        570        580        590        600 
LVGFGVAIAT ATQFIENFPE RTYKSMIIPL MQEDKRAGEA TRKGFYLYDD RRKAKPDPEI 

       610        620        630        640        650        660 
KNYIDKARSV SGAKPDPKLE KLSEKEIIEM TFFPVVNEAC RVFAEGIAVK AADLDIAGIF 

       670        680        690        700        710        720 
GMGFPPYRGG IMFWADSIGS KYIYSKLEEW SKAYGEFFKP CAFLAERGSK GAPLSAPLEQ 


SRSRL 

« Hide

References

[1]"A full-length cDNA clone from Brassica napus encoding a multifunctional enzyme of the glyoxysomal fatty acid beta-oxidation."
Geshi N., Rechinger K.B., Brandt A.
Plant Gene Register PGR98-067
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Global.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ000886 mRNA. Translation: CAA04386.1.
PIRT08017.

3D structure databases

ProteinModelPortalO49809.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEO49809.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00659.

Family and domain databases

Gene3D1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
InterProIPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR018376. Enoyl-CoA_hyd/isom_CS.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00725. 3HCDH. 1 hit.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMSSF48179. SSF48179. 2 hits.
PROSITEPS00067. 3HCDH. 1 hit.
PS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMFPA_BRANA
AccessionPrimary (citable) accession number: O49809
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: December 1, 2001
Last modified: February 19, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways