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O49809

- MFPA_BRANA

UniProt

O49809 - MFPA_BRANA

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Protein
Glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a
Gene
N/A
Organism
Brassica napus (Rape)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.By similarity
(3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.By similarity
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei119 – 1191Nucleophile Reviewed prediction
Active sitei139 – 1391Proton acceptor Reviewed prediction

GO - Molecular functioni

  1. 3-hydroxyacyl-CoA dehydratase activity Source: UniProtKB
  2. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-EC
  3. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB
  4. coenzyme binding Source: InterPro
  5. dodecenoyl-CoA delta-isomerase activity Source: UniProtKB
  6. enoyl-CoA hydratase activity Source: UniProtKB

GO - Biological processi

  1. fatty acid beta-oxidation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase, Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a
Including the following 2 domains:
Enoyl-CoA hydratase/3-2-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase (EC:4.2.1.17, EC:5.1.2.3, EC:5.3.3.8)
3-hydroxyacyl-CoA dehydrogenase (EC:1.1.1.35)
OrganismiBrassica napus (Rape)
Taxonomic identifieri3708 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Subcellular locationi

Glyoxysome By similarity

GO - Cellular componenti

  1. glyoxysome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 725725Glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a
PRO_0000109250Add
BLAST

Proteomic databases

PRIDEiO49809.

Structurei

3D structure databases

ProteinModelPortaliO49809.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi723 – 7253Microbody targeting signal Reviewed prediction

Domaini

The epimerase and isomerase activities are contained in the N-terminal region while the dehydrogenase activity is in the C-terminal region By similarity.By similarity

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR018376. Enoyl-CoA_hyd/isom_CS.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 1 hit.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
PROSITEiPS00067. 3HCDH. 1 hit.
PS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O49809-1 [UniParc]FASTAAdd to Basket

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MASRTKGTTT IEVGADGVAV ITLINPPVNS LSFDVLYSLK SNYEEALSRN    50
DVKAIVVTGA KGKFSGGFDI SGFGEIQKGT MKEPKVGYIS IDILTDLLEA 100
AKKPSVAAID GLALGGGLEL SMACHARISA PGAQLGLPEL QLGVIPGFGG 150
TQRLPRLVGL TKALEMILTS KPVKAEEGHS LGLIDAVVPP AELLNAARRW 200
ALDIAERRKP WVSSVLKTDK LPPLGEAREI LKFAKDQTRR QAPNMKHPLM 250
CLEAVEVGIV SGSRAGLEKE AQVGSEVINL DTTKGLIHVF FSQRGTTKVP 300
GVTDRGLVPR KINKVAIIGG GLMGSGIATA LILSNYSVIL KEVNEKFLEA 350
GIGRVKANLQ SRVKKGKMSK EKFEKTMSLL KGSLDYESFR DVDMVIEAVI 400
ENISLKQQIF ADLEKYCPQH CILASNTSTI DLNKIGERTK SQDRIIGAHF 450
FSPAHVMPLL EIVRTNHTSA QVIVDLLDVG KKIRKTPVVV GNCTGFAVNR 500
MFFPYTQAAM FLVEHGTDPY LIDKAVSKFG MPMGPFRLCD LVGFGVAIAT 550
ATQFIENFPE RTYKSMIIPL MQEDKRAGEA TRKGFYLYDD RRKAKPDPEI 600
KNYIDKARSV SGAKPDPKLE KLSEKEIIEM TFFPVVNEAC RVFAEGIAVK 650
AADLDIAGIF GMGFPPYRGG IMFWADSIGS KYIYSKLEEW SKAYGEFFKP 700
CAFLAERGSK GAPLSAPLEQ SRSRL 725
Length:725
Mass (Da):79,023
Last modified:December 1, 2001 - v2
Checksum:i6627C52D433EA976
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ000886 mRNA. Translation: CAA04386.1.
PIRiT08017.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ000886 mRNA. Translation: CAA04386.1 .
PIRi T08017.

3D structure databases

ProteinModelPortali O49809.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi O49809.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00659 .

Family and domain databases

Gene3Di 1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
InterProi IPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR018376. Enoyl-CoA_hyd/isom_CS.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00725. 3HCDH. 1 hit.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view ]
SUPFAMi SSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
PROSITEi PS00067. 3HCDH. 1 hit.
PS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A full-length cDNA clone from Brassica napus encoding a multifunctional enzyme of the glyoxysomal fatty acid beta-oxidation."
    Geshi N., Rechinger K.B., Brandt A.
    Plant Gene Register PGR98-067
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Global.

Entry informationi

Entry nameiMFPA_BRANA
AccessioniPrimary (citable) accession number: O49809
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: December 1, 2001
Last modified: June 11, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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