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O49809

- MFPA_BRANA

UniProt

O49809 - MFPA_BRANA

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Protein

Glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a

Gene
N/A
Organism
Brassica napus (Rape)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.By similarity
(3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.By similarity
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei119 – 1191NucleophileSequence Analysis
Active sitei139 – 1391Proton acceptorSequence Analysis

GO - Molecular functioni

  1. 3-hydroxyacyl-CoA dehydratase activity Source: UniProtKB
  2. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-EC
  3. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB
  4. coenzyme binding Source: InterPro
  5. dodecenoyl-CoA delta-isomerase activity Source: UniProtKB
  6. enoyl-CoA hydratase activity Source: UniProtKB

GO - Biological processi

  1. fatty acid beta-oxidation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase, Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a
Including the following 2 domains:
Enoyl-CoA hydratase/3-2-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase (EC:4.2.1.17, EC:5.1.2.3, EC:5.3.3.8)
3-hydroxyacyl-CoA dehydrogenase (EC:1.1.1.35)
OrganismiBrassica napus (Rape)Imported
Taxonomic identifieri3708 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Subcellular locationi

Glyoxysome By similarity

GO - Cellular componenti

  1. glyoxysome Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 725725Glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-aPRO_0000109250Add
BLAST

Proteomic databases

PRIDEiO49809.

Structurei

3D structure databases

ProteinModelPortaliO49809.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi723 – 7253Microbody targeting signalSequence Analysis

Domaini

The epimerase and isomerase activities are contained in the N-terminal region while the dehydrogenase activity is in the C-terminal region.By similarity

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.Curated
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.Curated

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR018376. Enoyl-CoA_hyd/isom_CS.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 1 hit.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
PROSITEiPS00067. 3HCDH. 1 hit.
PS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O49809-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASRTKGTTT IEVGADGVAV ITLINPPVNS LSFDVLYSLK SNYEEALSRN
60 70 80 90 100
DVKAIVVTGA KGKFSGGFDI SGFGEIQKGT MKEPKVGYIS IDILTDLLEA
110 120 130 140 150
AKKPSVAAID GLALGGGLEL SMACHARISA PGAQLGLPEL QLGVIPGFGG
160 170 180 190 200
TQRLPRLVGL TKALEMILTS KPVKAEEGHS LGLIDAVVPP AELLNAARRW
210 220 230 240 250
ALDIAERRKP WVSSVLKTDK LPPLGEAREI LKFAKDQTRR QAPNMKHPLM
260 270 280 290 300
CLEAVEVGIV SGSRAGLEKE AQVGSEVINL DTTKGLIHVF FSQRGTTKVP
310 320 330 340 350
GVTDRGLVPR KINKVAIIGG GLMGSGIATA LILSNYSVIL KEVNEKFLEA
360 370 380 390 400
GIGRVKANLQ SRVKKGKMSK EKFEKTMSLL KGSLDYESFR DVDMVIEAVI
410 420 430 440 450
ENISLKQQIF ADLEKYCPQH CILASNTSTI DLNKIGERTK SQDRIIGAHF
460 470 480 490 500
FSPAHVMPLL EIVRTNHTSA QVIVDLLDVG KKIRKTPVVV GNCTGFAVNR
510 520 530 540 550
MFFPYTQAAM FLVEHGTDPY LIDKAVSKFG MPMGPFRLCD LVGFGVAIAT
560 570 580 590 600
ATQFIENFPE RTYKSMIIPL MQEDKRAGEA TRKGFYLYDD RRKAKPDPEI
610 620 630 640 650
KNYIDKARSV SGAKPDPKLE KLSEKEIIEM TFFPVVNEAC RVFAEGIAVK
660 670 680 690 700
AADLDIAGIF GMGFPPYRGG IMFWADSIGS KYIYSKLEEW SKAYGEFFKP
710 720
CAFLAERGSK GAPLSAPLEQ SRSRL
Length:725
Mass (Da):79,023
Last modified:December 1, 2001 - v2
Checksum:i6627C52D433EA976
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ000886 mRNA. Translation: CAA04386.1.
PIRiT08017.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ000886 mRNA. Translation: CAA04386.1 .
PIRi T08017.

3D structure databases

ProteinModelPortali O49809.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi O49809.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00659 .

Family and domain databases

Gene3Di 1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
InterProi IPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR018376. Enoyl-CoA_hyd/isom_CS.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00725. 3HCDH. 1 hit.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view ]
SUPFAMi SSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
PROSITEi PS00067. 3HCDH. 1 hit.
PS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A full-length cDNA clone from Brassica napus encoding a multifunctional enzyme of the glyoxysomal fatty acid beta-oxidation."
    Geshi N., Rechinger K.B., Brandt A.
    Plant Gene Register PGR98-067
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Global.

Entry informationi

Entry nameiMFPA_BRANA
AccessioniPrimary (citable) accession number: O49809
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: December 1, 2001
Last modified: October 29, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3