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O49809

- MFPA_BRANA

UniProt

O49809 - MFPA_BRANA

Protein

Glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a

Gene
N/A
Organism
Brassica napus (Rape)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 2 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.By similarity
    (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.
    (S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.By similarity
    (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei119 – 1191NucleophileSequence Analysis
    Active sitei139 – 1391Proton acceptorSequence Analysis

    GO - Molecular functioni

    1. 3-hydroxyacyl-CoA dehydratase activity Source: UniProtKB
    2. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-EC
    3. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB
    4. coenzyme binding Source: InterPro
    5. dodecenoyl-CoA delta-isomerase activity Source: UniProtKB
    6. enoyl-CoA hydratase activity Source: UniProtKB

    GO - Biological processi

    1. fatty acid beta-oxidation Source: UniProtKB

    Keywords - Molecular functioni

    Isomerase, Lyase, Oxidoreductase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    UniPathwayiUPA00659.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a
    Including the following 2 domains:
    Enoyl-CoA hydratase/3-2-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase (EC:4.2.1.17, EC:5.1.2.3, EC:5.3.3.8)
    3-hydroxyacyl-CoA dehydrogenase (EC:1.1.1.35)
    OrganismiBrassica napus (Rape)Imported
    Taxonomic identifieri3708 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

    Subcellular locationi

    Glyoxysome By similarity

    GO - Cellular componenti

    1. glyoxysome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Glyoxysome, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 725725Glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-aPRO_0000109250Add
    BLAST

    Proteomic databases

    PRIDEiO49809.

    Structurei

    3D structure databases

    ProteinModelPortaliO49809.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi723 – 7253Microbody targeting signalSequence Analysis

    Domaini

    The epimerase and isomerase activities are contained in the N-terminal region while the dehydrogenase activity is in the C-terminal region.By similarity

    Sequence similaritiesi

    In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.Curated
    In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.Curated

    Family and domain databases

    Gene3Di1.10.1040.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.226.10. 1 hit.
    InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
    IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
    IPR006108. 3HC_DH_C.
    IPR008927. 6-PGluconate_DH_C-like.
    IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    IPR013328. DH_multihelical.
    IPR018376. Enoyl-CoA_hyd/isom_CS.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00725. 3HCDH. 1 hit.
    PF02737. 3HCDH_N. 1 hit.
    PF00378. ECH. 1 hit.
    [Graphical view]
    SUPFAMiSSF48179. SSF48179. 2 hits.
    SSF52096. SSF52096. 1 hit.
    PROSITEiPS00067. 3HCDH. 1 hit.
    PS00166. ENOYL_COA_HYDRATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O49809-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASRTKGTTT IEVGADGVAV ITLINPPVNS LSFDVLYSLK SNYEEALSRN    50
    DVKAIVVTGA KGKFSGGFDI SGFGEIQKGT MKEPKVGYIS IDILTDLLEA 100
    AKKPSVAAID GLALGGGLEL SMACHARISA PGAQLGLPEL QLGVIPGFGG 150
    TQRLPRLVGL TKALEMILTS KPVKAEEGHS LGLIDAVVPP AELLNAARRW 200
    ALDIAERRKP WVSSVLKTDK LPPLGEAREI LKFAKDQTRR QAPNMKHPLM 250
    CLEAVEVGIV SGSRAGLEKE AQVGSEVINL DTTKGLIHVF FSQRGTTKVP 300
    GVTDRGLVPR KINKVAIIGG GLMGSGIATA LILSNYSVIL KEVNEKFLEA 350
    GIGRVKANLQ SRVKKGKMSK EKFEKTMSLL KGSLDYESFR DVDMVIEAVI 400
    ENISLKQQIF ADLEKYCPQH CILASNTSTI DLNKIGERTK SQDRIIGAHF 450
    FSPAHVMPLL EIVRTNHTSA QVIVDLLDVG KKIRKTPVVV GNCTGFAVNR 500
    MFFPYTQAAM FLVEHGTDPY LIDKAVSKFG MPMGPFRLCD LVGFGVAIAT 550
    ATQFIENFPE RTYKSMIIPL MQEDKRAGEA TRKGFYLYDD RRKAKPDPEI 600
    KNYIDKARSV SGAKPDPKLE KLSEKEIIEM TFFPVVNEAC RVFAEGIAVK 650
    AADLDIAGIF GMGFPPYRGG IMFWADSIGS KYIYSKLEEW SKAYGEFFKP 700
    CAFLAERGSK GAPLSAPLEQ SRSRL 725
    Length:725
    Mass (Da):79,023
    Last modified:December 1, 2001 - v2
    Checksum:i6627C52D433EA976
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ000886 mRNA. Translation: CAA04386.1.
    PIRiT08017.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ000886 mRNA. Translation: CAA04386.1 .
    PIRi T08017.

    3D structure databases

    ProteinModelPortali O49809.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi O49809.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00659 .

    Family and domain databases

    Gene3Di 1.10.1040.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.226.10. 1 hit.
    InterProi IPR006180. 3-OHacyl-CoA_DH_CS.
    IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
    IPR006108. 3HC_DH_C.
    IPR008927. 6-PGluconate_DH_C-like.
    IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    IPR013328. DH_multihelical.
    IPR018376. Enoyl-CoA_hyd/isom_CS.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00725. 3HCDH. 1 hit.
    PF02737. 3HCDH_N. 1 hit.
    PF00378. ECH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48179. SSF48179. 2 hits.
    SSF52096. SSF52096. 1 hit.
    PROSITEi PS00067. 3HCDH. 1 hit.
    PS00166. ENOYL_COA_HYDRATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A full-length cDNA clone from Brassica napus encoding a multifunctional enzyme of the glyoxysomal fatty acid beta-oxidation."
      Geshi N., Rechinger K.B., Brandt A.
      Plant Gene Register PGR98-067
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Global.

    Entry informationi

    Entry nameiMFPA_BRANA
    AccessioniPrimary (citable) accession number: O49809
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2003
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 78 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3