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Protein

Probable tRNA N6-adenosine threonylcarbamoyltransferase

Gene

GCP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N6-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

a divalent metal cationUniRule annotationNote: Binds 1 divalent metal cation per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi113Divalent metal cationUniRule annotation1
Metal bindingi117Divalent metal cationUniRule annotation1
Metal bindingi134Divalent metal cationUniRule annotation1
Binding sitei166SubstrateUniRule annotation1
Binding sitei181Substrate; via amide nitrogenUniRule annotation1
Binding sitei185SubstrateUniRule annotation1
Binding sitei270SubstrateUniRule annotation1
Metal bindingi298Divalent metal cationUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferaseUniRule annotation, Transferase
Biological processtRNA processingUniRule annotation
LigandMetal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciARA:AT4G22720-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
Glycoprotease 2UniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein GCP2UniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein GCP2UniRule annotation
Gene namesi
Name:GCP2UniRule annotation
Ordered Locus Names:At4g22720Imported
ORF Names:T12H17.110Imported, T12H17_110Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRilocus:2132624. AT4G22720.

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Nucleus UniRule annotation

GO - Cellular componenti

  • cytosol Source: TAIR
  • EKC/KEOPS complex Source: GO_Central
  • nucleus Source: UniProtKB-SubCell

Keywords - Cellular componenti

CytoplasmUniRule annotation, NucleusUniRule annotation

Interactioni

Subunit structurei

Component of the EKC/KEOPS complex; the whole complex dimerizes.UniRule annotation

Protein-protein interaction databases

IntActiO49653. 13 interactors.
STRINGi3702.AT4G22720.1.

Structurei

3D structure databases

SMRiO49653.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 305Peptidase_M22InterPro annotationAdd BLAST277

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni134 – 138Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2708. Eukaryota.
COG0533. LUCA.
HOGENOMiHOG000109569.
KOiK01409.
OMAiAHAGAEM.
OrthoDBiEOG09360DJQ.

Family and domain databases

HAMAPiMF_01446. Kae1. 1 hit.
InterProiView protein in InterPro
IPR000905. Gcp-like_dom.
IPR034680. Kae1/OSGEP.
IPR017861. KAE1/TsaD.
PfamiView protein in Pfam
PF00814. Peptidase_M22. 1 hit.
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.

Sequencei

Sequence statusi: Complete.

O49653-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKKMIAIGF EGSANKIGVG IVTLDGTILA NPRHTYITPP GHGFLPRETA
60 70 80 90 100
HHHLDHVLPL VKSALETSQV TPEEIDCICY TKGPGMGAPL QVSAIVVRVL
110 120 130 140 150
SQLWKKPIVA VNHCVAHIEM GRVVTGADDP VVLYVSGGNT QVIAYSEGRY
160 170 180 190 200
RIFGETIDIA VGNCLDRFAR VLKLSNDPSP GYNIEQLAKK GENFIDLPYA
210 220 230 240 250
VKGMDVSFSG ILSYIETTAE EKLKNNECTP ADLCYSLQET VFAMLVEITE
260 270 280 290 300
RAMAHCDKKD VLIVGGVGCN ERLQEMMRTM CSERDGKLFA TDDRYCIDNG
310 320 330 340 350
AMIAYTGLLA FVNGIETPIE DSTFTQRFRT DEVHAVWREK EAVLLGDKKV

AAN
Length:353
Mass (Da):38,768
Last modified:June 1, 1998 - v1
Checksum:i2A9AAB7515936E76
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY050841 mRNA. Translation: AAK92776.1.
AY079379 mRNA. Translation: AAL85110.1.
AY088084 mRNA. Translation: AAM65630.1.
CP002687 Genomic DNA. Translation: AEE84643.1.
CP002687 Genomic DNA. Translation: AEE84644.1.
AL021635 Genomic DNA. Translation: CAA16557.1.
AL161557 Genomic DNA. Translation: CAB79227.1.
PIRiT04567.
RefSeqiNP_194003.1. NM_118398.5.
NP_974593.1. NM_202864.1.
UniGeneiAt.1865.

Genome annotation databases

EnsemblPlantsiAT4G22720.1; AT4G22720.1; AT4G22720.
AT4G22720.2; AT4G22720.2; AT4G22720.
GeneIDi828368.
GrameneiAT4G22720.1; AT4G22720.1; AT4G22720.
AT4G22720.2; AT4G22720.2; AT4G22720.
KEGGiath:AT4G22720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY050841 mRNA. Translation: AAK92776.1.
AY079379 mRNA. Translation: AAL85110.1.
AY088084 mRNA. Translation: AAM65630.1.
CP002687 Genomic DNA. Translation: AEE84643.1.
CP002687 Genomic DNA. Translation: AEE84644.1.
AL021635 Genomic DNA. Translation: CAA16557.1.
AL161557 Genomic DNA. Translation: CAB79227.1.
PIRiT04567.
RefSeqiNP_194003.1. NM_118398.5.
NP_974593.1. NM_202864.1.
UniGeneiAt.1865.

3D structure databases

SMRiO49653.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO49653. 13 interactors.
STRINGi3702.AT4G22720.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G22720.1; AT4G22720.1; AT4G22720.
AT4G22720.2; AT4G22720.2; AT4G22720.
GeneIDi828368.
GrameneiAT4G22720.1; AT4G22720.1; AT4G22720.
AT4G22720.2; AT4G22720.2; AT4G22720.
KEGGiath:AT4G22720.

Organism-specific databases

AraportiAT4G22720.
TAIRilocus:2132624. AT4G22720.

Phylogenomic databases

eggNOGiKOG2708. Eukaryota.
COG0533. LUCA.
HOGENOMiHOG000109569.
KOiK01409.
OMAiAHAGAEM.
OrthoDBiEOG09360DJQ.

Enzyme and pathway databases

BioCyciARA:AT4G22720-MONOMER.

Family and domain databases

HAMAPiMF_01446. Kae1. 1 hit.
InterProiView protein in InterPro
IPR000905. Gcp-like_dom.
IPR034680. Kae1/OSGEP.
IPR017861. KAE1/TsaD.
PfamiView protein in Pfam
PF00814. Peptidase_M22. 1 hit.
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiO49653_ARATH
AccessioniPrimary (citable) accession number: O49653
Entry historyiIntegrated into UniProtKB/TrEMBL: June 1, 1998
Last sequence update: June 1, 1998
Last modified: June 7, 2017
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.