O49623 (DPNP2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
SAL2 phosphatase

Alternative name(s):
3'(2'),5'-bisphosphate nucleotidase 2
EC=3.1.3.7
3'(2'),5'-bisphosphonucleoside 3'(2')-phosphohydrolase 2
DPNPase 2
Inositol polyphosphate 1-phosphatase 2
Short name=IPPase 2
Inositol-1,4-bisphosphate 1-phosphatase 2
EC=3.1.3.57

Gene names

Name:	SAL2
Ordered Locus Names:	At5g64000
ORF Names:	MBM17.10

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'- phosphate (PAP) to AMP. May regulate the flux of sulfur in the sulfur-activation pathway by converting PAPS to APS By similarity. Prevents both the toxicity of PAP on RNA processing enzymes as well as the product inhibition by PAP of sulfate conjugation. Is also able to hydrolyze inositol 1,4-bisphosphate. Ref.1
Catalytic activity	Adenosine 3',5'-bisphosphate + H2O = adenosine 5'-phosphate + phosphate. 1D-myo-inositol 1,4-bisphosphate + H2O = 1D-myo-inositol 4-phosphate + phosphate.
Cofactor	Magnesium.
Enzyme regulation	Inhibited by Li+ (IC50=10 millimolar), Na+ (IC50=200 millimolar) and Ca2+ (IC50=0.03 millimolar).
Pathway	Signal transduction; phosphatidylinositol signaling pathway.
Tissue specificity	Very low expression in roots, leaves, stems, flowers and siliques.
Miscellaneous	Substrate preference is 3'-phosphoadenosine 5'-phosphate (PAP) > adenosine 3'-phosphate 5'-phosphosulfate (PAPS)>> inositol-1,4-bisphosphate. No activity observed against 3' or 5'-AMP, inositol monophosphate and fructose-1,6-bisphosphate.
Sequence similarities	Belongs to the inositol monophosphatase family.

Ontologies

Keywords
Ligand	Calcium Lithium Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	sulfur compound metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	3'(2'),5'-bisphosphate nucleotidase activity Inferred from direct assay Ref.1. Source: TAIR inositol-1,4-bisphosphate 1-phosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	SAL2 phosphatase		PRO_0000142531
Regions
	Region	136 – 139	4	Substrate binding By similarity
Sites
	Metal binding	71	1	Magnesium 1 By similarity
	Metal binding	134	1	Magnesium 1 By similarity
	Metal binding	134	1	Magnesium 2 By similarity
	Metal binding	136	1	Magnesium 1; via carbonyl oxygen By similarity
	Metal binding	137	1	Magnesium 2 By similarity
	Metal binding	284	1	Magnesium 2 By similarity
	Binding site	71	1	Substrate By similarity
	Binding site	284	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O49623 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 7F82E8EB4FAAF8FE	FASTA	347	37,509
	10 20 30 40 50 60 MSYEKELAAA KKAVTLAARL SQEVQKTLLQ SQVWKKSDRS PVTAADYGSQ AVVSLVLERE 70 80 90 100 110 120 LQPDKLSLVA EEETGDLRKN GSEAFLEDIA KLVKDTLASE ESYTSSPLST DDVLNAIDCG 130 140 150 160 170 180 KSEGGCKGSH WVLDPIDGTR GFVRGEQYAV GLALLVEGKV VLGVMACPNL PLASAVCATD 190 200 210 220 230 240 NSSQEDVGCL FFATTGSGTY VQSLKGNSLP QKVQVSSNEN LDEAKFLESY HKPIPIHGTI 250 260 270 280 290 300 AKKLGIKALP VRIDSQAKYA ALSRGDAEIY LRFTLNGYRE CIWDHAPGSI ITTEAGGVVC 310 320 330 340 DATGKSLDFS KGKYLAHKTG IIVTTKKLKP WILKAVRESI EEENLYF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Arabidopsis HAL2-like gene family includes a novel sodium-sensitive phosphatase." Gil-Mascarell R., Lopez-Coronado J.M., Belles J.M., Serrano R., Rodriguez P.L. Plant J. 17:373-383(1999) [PubMed: 10205895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed: 10718197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z83312 mRNA. Translation: CAB05889.1. AB019227 Genomic DNA. Translation: BAA96903.1. CP002688 Genomic DNA. Translation: AED97828.1. AY070383 mRNA. Translation: AAL49879.1. AY096575 mRNA. Translation: AAM20225.1.
IPI	IPI00523680.
RefSeq	NP_201205.1. NM_125796.4.
UniGene	At.181. At.28969.
3D structure databases
ProteinModelPortal	O49623.
SMR	O49623. Positions 2-342.
ModBase	Search...
Protein-protein interaction databases
STRING	O49623.
Proteomic databases
PRIDE	O49623.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G64000.1; AT5G64000.1; AT5G64000.
GeneID	836521.
GenomeReviews	Gene locus AT5G64000 in contig BA000015_GR.
KEGG	ath:AT5G64000.
NMPDR	fig|3702.1.peg.28456.
Organism-specific databases
GeneFarm	2321.
TAIR	At5g64000.
Phylogenomic databases
eggNOG	KOG1528.
GeneTree	EPGT00070000029602.
HOGENOM	HBG599916.
InParanoid	O49623.
OMA	IHGTIAK.
PhylomeDB	O49623.
ProtClustDB	CLSN2914821.
Gene expression databases
ArrayExpress	O49623.
Genevestigator	O49623.
GermOnline	AT5G64000. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR006239. Bisphos_HAL2. IPR020583. Inositol_monoP_metal-BS. IPR000760. Inositol_monophosphatase. [Graphical view]
KO	K15422.
PANTHER	PTHR20854. Inositol_P. 1 hit.
Pfam	PF00459. Inositol_P. 1 hit. [Graphical view]
TIGRFAMs	TIGR01330. Bisphos_HAL2. 1 hit.
PROSITE	PS00629. IMP_1. 1 hit. PS00630. IMP_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DPNP2_ARATH

Accession

Primary (citable) accession number: O49623

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2003
Last sequence update:	June 1, 1998
Last modified:	November 16, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program