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Protein

SAL2 phosphatase

Gene

SAL2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'- phosphate (PAP) to AMP. May regulate the flux of sulfur in the sulfur-activation pathway by converting PAPS to APS (By similarity). Prevents both the toxicity of PAP on RNA processing enzymes as well as the product inhibition by PAP of sulfate conjugation. Is also able to hydrolyze inositol 1,4-bisphosphate.By similarity1 Publication

Catalytic activityi

Adenosine 3',5'-bisphosphate + H2O = adenosine 5'-phosphate + phosphate.
1D-myo-inositol 1,4-bisphosphate + H2O = 1D-myo-inositol 4-phosphate + phosphate.

Cofactori

Enzyme regulationi

Inhibited by Li+ (IC50=10 millimolar), Na+ (IC50=200 millimolar) and Ca2+ (IC50=0.03 millimolar).

Pathwayi: phosphatidylinositol signaling pathway

This protein is involved in the pathway phosphatidylinositol signaling pathway, which is part of Signal transduction.
View all proteins of this organism that are known to be involved in the pathway phosphatidylinositol signaling pathway and in Signal transduction.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi71Magnesium 1By similarity1
Binding sitei71SubstrateBy similarity1
Metal bindingi134Magnesium 1By similarity1
Metal bindingi134Magnesium 2By similarity1
Metal bindingi136Magnesium 1; via carbonyl oxygenBy similarity1
Metal bindingi137Magnesium 2By similarity1
Metal bindingi284Magnesium 2By similarity1
Binding sitei284SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calcium, Lithium, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G64000-MONOMER.
UniPathwayiUPA00944.

Names & Taxonomyi

Protein namesi
Recommended name:
SAL2 phosphatase
Alternative name(s):
3'(2'),5'-bisphosphate nucleotidase 2 (EC:3.1.3.7)
3'(2'),5'-bisphosphonucleoside 3'(2')-phosphohydrolase 2
DPNPase 2
Inositol polyphosphate 1-phosphatase 2
Short name:
IPPase 2
Inositol-1,4-bisphosphate 1-phosphatase 2 (EC:3.1.3.57)
Gene namesi
Name:SAL2
Ordered Locus Names:At5g64000
ORF Names:MBM17.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G64000.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001425311 – 347SAL2 phosphataseAdd BLAST347

Proteomic databases

PaxDbiO49623.

Expressioni

Tissue specificityi

Very low expression in roots, leaves, stems, flowers and siliques.

Gene expression databases

GenevisibleiO49623. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G64000.1.

Structurei

3D structure databases

ProteinModelPortaliO49623.
SMRiO49623.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni136 – 139Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the inositol monophosphatase family.Curated

Phylogenomic databases

eggNOGiKOG1528. Eukaryota.
COG1218. LUCA.
HOGENOMiHOG000170673.
InParanoidiO49623.
KOiK15422.
OMAiSYHKPIP.
OrthoDBiEOG09360EFU.
PhylomeDBiO49623.

Family and domain databases

InterProiIPR006239. Bisphos_HAL2.
IPR020583. Inositol_monoP_metal-BS.
IPR000760. Inositol_monophosphatase-like.
[Graphical view]
PANTHERiPTHR20854. PTHR20854. 3 hits.
PfamiPF00459. Inositol_P. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01330. bisphos_HAL2. 1 hit.
PROSITEiPS00629. IMP_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O49623-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYEKELAAA KKAVTLAARL SQEVQKTLLQ SQVWKKSDRS PVTAADYGSQ
60 70 80 90 100
AVVSLVLERE LQPDKLSLVA EEETGDLRKN GSEAFLEDIA KLVKDTLASE
110 120 130 140 150
ESYTSSPLST DDVLNAIDCG KSEGGCKGSH WVLDPIDGTR GFVRGEQYAV
160 170 180 190 200
GLALLVEGKV VLGVMACPNL PLASAVCATD NSSQEDVGCL FFATTGSGTY
210 220 230 240 250
VQSLKGNSLP QKVQVSSNEN LDEAKFLESY HKPIPIHGTI AKKLGIKALP
260 270 280 290 300
VRIDSQAKYA ALSRGDAEIY LRFTLNGYRE CIWDHAPGSI ITTEAGGVVC
310 320 330 340
DATGKSLDFS KGKYLAHKTG IIVTTKKLKP WILKAVRESI EEENLYF
Length:347
Mass (Da):37,509
Last modified:June 1, 1998 - v1
Checksum:i7F82E8EB4FAAF8FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z83312 mRNA. Translation: CAB05889.1.
AB019227 Genomic DNA. Translation: BAA96903.1.
CP002688 Genomic DNA. Translation: AED97828.1.
AY070383 mRNA. Translation: AAL49879.1.
AY096575 mRNA. Translation: AAM20225.1.
RefSeqiNP_201205.1. NM_125796.5.
UniGeneiAt.181.
At.28969.

Genome annotation databases

EnsemblPlantsiAT5G64000.1; AT5G64000.1; AT5G64000.
GeneIDi836521.
GrameneiAT5G64000.1; AT5G64000.1; AT5G64000.
KEGGiath:AT5G64000.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z83312 mRNA. Translation: CAB05889.1.
AB019227 Genomic DNA. Translation: BAA96903.1.
CP002688 Genomic DNA. Translation: AED97828.1.
AY070383 mRNA. Translation: AAL49879.1.
AY096575 mRNA. Translation: AAM20225.1.
RefSeqiNP_201205.1. NM_125796.5.
UniGeneiAt.181.
At.28969.

3D structure databases

ProteinModelPortaliO49623.
SMRiO49623.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G64000.1.

Proteomic databases

PaxDbiO49623.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G64000.1; AT5G64000.1; AT5G64000.
GeneIDi836521.
GrameneiAT5G64000.1; AT5G64000.1; AT5G64000.
KEGGiath:AT5G64000.

Organism-specific databases

TAIRiAT5G64000.

Phylogenomic databases

eggNOGiKOG1528. Eukaryota.
COG1218. LUCA.
HOGENOMiHOG000170673.
InParanoidiO49623.
KOiK15422.
OMAiSYHKPIP.
OrthoDBiEOG09360EFU.
PhylomeDBiO49623.

Enzyme and pathway databases

UniPathwayiUPA00944.
BioCyciARA:AT5G64000-MONOMER.

Miscellaneous databases

PROiO49623.

Gene expression databases

GenevisibleiO49623. AT.

Family and domain databases

InterProiIPR006239. Bisphos_HAL2.
IPR020583. Inositol_monoP_metal-BS.
IPR000760. Inositol_monophosphatase-like.
[Graphical view]
PANTHERiPTHR20854. PTHR20854. 3 hits.
PfamiPF00459. Inositol_P. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01330. bisphos_HAL2. 1 hit.
PROSITEiPS00629. IMP_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDPNP2_ARATH
AccessioniPrimary (citable) accession number: O49623
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: June 1, 1998
Last modified: November 30, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Substrate preference is 3'-phosphoadenosine 5'-phosphate (PAP) > adenosine 3'-phosphate 5'-phosphosulfate (PAPS)>> inositol 1,4-bisphosphate. No activity observed against 3' or 5'-AMP, inositol monophosphate and fructose-1,6-bisphosphate.

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.