ID   G2OX8_ARATH             Reviewed;         293 AA.
AC   O49561;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   16-JUN-2009, entry version 49.
DE   RecName: Full=Gibberellin 2-beta-dioxygenase 8;
DE            EC=1.14.11.13;
DE   AltName: Full=Gibberellin 2-beta-hydroxylase 8;
DE   AltName: Full=Gibberellin 2-oxidase 8;
DE   AltName: Full=GA 2-oxidase 8;
GN   Name=GA2OX7; OrderedLocusNames=At4g21200; ORFNames=F7J7.140;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=20083488; PubMed=10617198; DOI=10.1038/47134;
RA   Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G.,
RA   Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N.,
RA   Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M.,
RA   Weichselgartner M., de Simone V., Obermaier B., Mache R., Mueller M.,
RA   Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T.,
RA   Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I.,
RA   Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P.,
RA   Langham S.-A., McCullagh B., Bilham L., Robben J.,
RA   van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F.,
RA   Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E.,
RA   Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA   Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W.,
RA   Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P.,
RA   Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H.,
RA   De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R.,
RA   van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S.,
RA   Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R.,
RA   Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S.,
RA   Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H.,
RA   Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S.,
RA   Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A.,
RA   Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA   Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R.,
RA   Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S.,
RA   Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E.,
RA   Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A.,
RA   Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T.,
RA   Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C.,
RA   Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S.,
RA   Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K.,
RA   Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L.,
RA   Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J.,
RA   Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J.,
RA   Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D.,
RA   Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA   Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA   Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D.,
RA   Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C.,
RA   Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C.,
RA   Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R.,
RA   Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S.,
RA   Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A.,
RA   Chen E., Marra M.A., Martienssen R., McCombie W.R.;
RT   "Sequence and analysis of chromosome 4 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 402:769-777(1999).
RN   [2]
RP   FUNCTION, AND CHARACTERIZATION.
RX   MEDLINE=22397961; PubMed=12509528; DOI=10.1105/tpc.005975;
RA   Schomburg F.M., Bizzell C.M., Lee D.J., Zeevaart J.A.D., Amasino R.M.;
RT   "Overexpression of a novel class of gibberellin 2-oxidases decreases
RT   gibberellin levels and creates dwarf plants.";
RL   Plant Cell 15:151-163(2003).
CC   -!- FUNCTION: Catalyzes the 2-beta-hydroxylation of gibberellins (GA)
CC       precursors, rendering them unable to be converted to active GAs.
CC       Hydroxylates the C20-GA GA12 and GA53, but is not active on C19-
CC       GAs, like GA1, GA4, GA9 and GA20.
CC   -!- CATALYTIC ACTIVITY: Gibberellin 1 + 2-oxoglutarate + O(2) = 2-
CC       beta-hydroxygibberellin 1 + succinate + CO(2).
CC   -!- COFACTOR: Iron (By similarity).
CC   -!- PATHWAY: Plant hormone biosynthesis; gibberellin biosynthesis.
CC   -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC       family. GA2OX subfamily.
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DR   EMBL; AL021960; CAA17539.1; -; Genomic_DNA.
DR   EMBL; AL161554; CAB79120.1; -; Genomic_DNA.
DR   IPI; IPI00852244; -.
DR   PIR; T04951; T04951.
DR   GenomeReviews; CT486007_GR; AT4G21200.
DR   NMPDR; fig|3702.1.peg.19957; -.
DR   TAIR; At4g21200; -.
DR   OMA; O49561; WSEAYHI.
DR   BRENDA; 1.14.11.13; 302.
DR   ArrayExpress; O49561; -.
DR   GO; GO:0045543; F:gibberellin 2-beta-dioxygenase activity; IDA:TAIR.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016702; F:oxidoreductase activity, acting on single d...; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR005123; Oxoglutarate/Fe-dep_Oase.
DR   Pfam; PF03171; 2OG-FeII_Oxy; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Dioxygenase; Iron; Metal-binding; Oxidoreductase.
FT   CHAIN         1    293       Gibberellin 2-beta-dioxygenase 8.
FT                                /FTId=PRO_0000067309.
FT   ACT_SITE    236    236       Potential.
FT   METAL       170    170       Iron (By similarity).
FT   METAL       172    172       Iron (By similarity).
FT   METAL       226    226       Iron (By similarity).
SQ   SEQUENCE   293 AA;  34050 MW;  5138093F136DF6F6 CRC64;
     MDPPFNEIYN NLLYNQITKK DNDVSEIPFS FSVTAVVEEV ELPVIDVSRL IDGAEEEREK
     CKEAIARASR EWGFFQVINH GISMDVLEKM RQEQIRVFRE PFDKKSNSTM EKFASESEAL
     AYMLAEVLAE KSGQNSSFFK ENCVRNTCYL RMNRYPPCPK PSEVYGLMPH TDSDFLTILY
     QDQVGGLQLI KDNRWIAVKP NPKALIINIG DLFQAWSNGM YKSVEHRVMT NPKVERFSTA
     YFMCPSYDAV IECSSDRPAY RNFSFREFRQ QVQEDVKKFG FKVGLPRFLN HVY
//