O49561 (G2OX8_ARATH) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 70.
History...
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Gibberellin 2-beta-dioxygenase 8 EC=1.14.11.13 Alternative name(s): GA 2-oxidase 8 Gibberellin 2-beta-hydroxylase 8 Gibberellin 2-oxidase 8 | ||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) | ||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Protein attributes
| Sequence length | 338 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Catalyzes the 2-beta-hydroxylation of gibberellins (GA) precursors, rendering them unable to be converted to active GAs. Hydroxylates the C20-GA GA12 and GA53, but is not active on C19-GAs, like GA1, GA4, GA9 and GA20. Ref.4 |
| Catalytic activity | Gibberellin 1 + 2-oxoglutarate + O2 = 2-beta-hydroxygibberellin 1 + succinate + CO2. |
| Cofactor | Iron By similarity. |
| Pathway | |
| Sequence similarities | Belongs to the iron/ascorbate-dependent oxidoreductase family. GA2OX subfamily. Contains 1 Fe2OG dioxygenase domain. |
| Sequence caution | The sequence ABK28642.1 differs from that shown. Reason: Erroneous termination at position 339. Translated as stop. The sequence CAA17539.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB79120.1 differs from that shown. Reason: Erroneous gene model prediction. |
Ontologies
| Keywords | |
|---|---|
| Coding sequence diversity | Alternative splicing |
| Ligand | Iron Metal-binding |
| Molecular function | Dioxygenase Oxidoreductase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | gibberellin metabolic process Inferred from direct assay Ref.4. Source: TAIR |
| Molecular function | C-20 gibberellin 2-beta-dioxygenase activity Inferred from direct assay Ref.4. Source: TAIR metal ion bindingInferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygenInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Alternative products
| This entry describes 1 isoform produced by alternative splicing. [Select] Note: A number of isoforms are produced. According to EST sequences. | ||||||
| Isoform 1 (identifier: O49561-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 338 | 338 | Gibberellin 2-beta-dioxygenase 8 | PRO_0000067309 | |||||
Regions | |||||||||
| Domain | 191 – 290 | 100 | Fe2OG dioxygenase | ||||||
Sites | |||||||||
| Active site | 281 | 1 | Potential | ||||||
| Metal binding | 215 | 1 | Iron By similarity | ||||||
| Metal binding | 217 | 1 | Iron By similarity | ||||||
| Metal binding | 271 | 1 | Iron By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. McCombie W.R.Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [2] | The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia. |
| [3] | "Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations." Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P. Plant Biotechnol. J. 4:317-324(2006) [PubMed: 17147637] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [4] | "Overexpression of a novel class of gibberellin 2-oxidases decreases gibberellin levels and creates dwarf plants." Schomburg F.M., Bizzell C.M., Lee D.J., Zeevaart J.A.D., Amasino R.M. Plant Cell 15:151-163(2003) [PubMed: 12509528] [Abstract] Cited for: FUNCTION, CHARACTERIZATION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AL021960 Genomic DNA. Translation: CAA17539.1. Sequence problems. AL161554 Genomic DNA. Translation: CAB79120.1. Sequence problems. CP002687 Genomic DNA. Translation: AEE84420.1. DQ446856 mRNA. Translation: ABE66080.1. DQ653213 mRNA. Translation: ABK28642.1. Sequence problems. |
| IPI | IPI00852244. |
| PIR | T04951. |
| RefSeq | NP_193852.2. NM_118239.2. |
| UniGene | At.51238. |
3D structure databases | |
| ProteinModelPortal | O49561. |
| SMR | O49561. Positions 11-316. |
| ModBase | Search... |
Proteomic databases | |
| PRIDE | O49561. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblPlants | AT4G21200.1; AT4G21200.1; AT4G21200. |
| GeneID | 827868. |
| GenomeReviews | Gene locus AT4G21200 in contig CT486007_GR. |
| KEGG | ath:AT4G21200. |
| NMPDR | fig|3702.1.peg.19957. |
Organism-specific databases | |
| TAIR | At4g21200. |
Phylogenomic databases | |
| GeneTree | EPGT00050000009705. |
| HOGENOM | HBG592995. |
| InParanoid | O49561. |
| PhylomeDB | O49561. |
| ProtClustDB | CLSN2920296. |
Gene expression databases | |
| ArrayExpress | O49561. |
| Genevestigator | O49561. |
Family and domain databases | |
| InterPro | IPR005123. Oxoglutarate/Fe-dep_oxygenase. [Graphical view] |
| Pfam | PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view] |
| PROSITE | PS51471. FE2OG_OXY. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | G2OX8_ARATH | ||||||||
| Accession | Primary (citable) accession number: O49561 Secondary accession number(s): A0MF87, Q1PE62 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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