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Protein

Cysteine desulfurase, mitochondrial

Gene

NIFS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the removal of elemental sulfur from cysteine to produce alanine. Supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters.2 Publications

Catalytic activityi

L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor.1 Publication

Cofactori

pyridoxal 5'-phosphate1 Publication

Enzyme regulationi

Threefold increase in the catalytic activity in the presence of FH (frataxin) (PubMed:22511606). 30-fold increase in the catalytic activity in the presence of SUFE1 (PubMed:16437155).2 Publications

Kineticsi

    1. Vmax=21.2 nmol/min/mg enzyme with cysteine as substrate1 Publication

    pH dependencei

    Optimum pH is 8.5.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei203Pyridoxal phosphateBy similarity1
    Binding sitei231Pyridoxal phosphateBy similarity1
    Binding sitei291Pyridoxal phosphateBy similarity1
    Active sitei377Cysteine persulfide intermediateBy similarity1
    Metal bindingi377Iron-sulfur (2Fe-2S); via persulfide groupBy similarity1

    GO - Molecular functioni

    • ATP binding Source: TAIR
    • cysteine desulfurase activity Source: TAIR
    • iron-sulfur cluster binding Source: UniProtKB-KW
    • pyridoxal phosphate binding Source: InterPro
    • zinc ion binding Source: TAIR

    GO - Biological processi

    • [2Fe-2S] cluster assembly Source: InterPro
    • iron-sulfur cluster assembly Source: TAIR

    Keywordsi

    Molecular functionTransferase
    LigandIron, Iron-sulfur, Metal-binding, Pyridoxal phosphate

    Enzyme and pathway databases

    BRENDAi2.8.1.6 399
    2.8.1.7 399
    ReactomeiR-ATH-1362409 Mitochondrial iron-sulfur cluster biogenesis
    R-ATH-947581 Molybdenum cofactor biosynthesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cysteine desulfurase, mitochondrial (EC:2.8.1.7)
    Alternative name(s):
    NIFS homolog 11 Publication
    Short name:
    AtNIFS1
    Protein AtMtNifS
    Gene namesi
    Name:NIFS11 Publication
    Synonyms:MtNIFS1, NIFS, NSF11 Publication
    Ordered Locus Names:At5g65720Imported
    ORF Names:F6H11.150Imported, MPA24.7Imported
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    AraportiAT5G65720
    TAIRilocus:2169985 AT5G65720

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Disruption phenotypei

    Lethal when homozygous.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 34MitochondrionSequence analysisAdd BLAST34
    ChainiPRO_000000129935 – 453Cysteine desulfurase, mitochondrialAdd BLAST419

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei254N6-(pyridoxal phosphate)lysineBy similarity1

    Proteomic databases

    PaxDbiO49543
    PRIDEiO49543

    Expressioni

    Gene expression databases

    ExpressionAtlasiO49543 baseline and differential
    GenevisibleiO49543 AT

    Interactioni

    Subunit structurei

    Interacts with FH (PubMed:22511606). Interacts with SUFE1 (PubMed:16437155).2 Publications

    Protein-protein interaction databases

    BioGridi21943, 2 interactors
    STRINGi3702.AT5G65720.1

    Structurei

    3D structure databases

    ProteinModelPortaliO49543
    SMRiO49543
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni123 – 124Pyridoxal phosphate bindingBy similarity2
    Regioni251 – 253Pyridoxal phosphate bindingBy similarity3

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiKOG1549 Eukaryota
    COG1104 LUCA
    HOGENOMiHOG000017510
    InParanoidiO49543
    KOiK04487
    OMAiEPIQSGG
    OrthoDBiEOG09360A7M
    PhylomeDBiO49543

    Family and domain databases

    Gene3Di3.40.640.10, 1 hit
    3.90.1150.10, 2 hits
    HAMAPiMF_00331 Cys_desulf_IscS, 1 hit
    InterProiView protein in InterPro
    IPR000192 Aminotrans_V_dom
    IPR010240 Cys_deSase_IscS
    IPR016454 Cysteine_dSase
    IPR015424 PyrdxlP-dep_Trfase
    IPR015422 PyrdxlP-dep_Trfase_dom1
    IPR015421 PyrdxlP-dep_Trfase_major
    PANTHERiPTHR11601:SF34 PTHR11601:SF34, 1 hit
    PfamiView protein in Pfam
    PF00266 Aminotran_5, 1 hit
    PIRSFiPIRSF005572 NifS, 1 hit
    SUPFAMiSSF53383 SSF53383, 1 hit
    TIGRFAMsiTIGR02006 IscS, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

    Note: A number of isoforms are produced. According to EST sequences.
    Isoform 1 (identifier: O49543-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MASKVISATI RRTLTKPHGT FSRCRYLSTA AAATEVNYED ESIMMKGVRI
    60 70 80 90 100
    SGRPLYLDMQ ATTPIDPRVF DAMNASQIHE YGNPHSRTHL YGWEAENAVE
    110 120 130 140 150
    NARNQVAKLI EASPKEIVFV SGATEANNMA VKGVMHFYKD TKKHVITTQT
    160 170 180 190 200
    EHKCVLDSCR HLQQEGFEVT YLPVKTDGLV DLEMLREAIR PDTGLVSIMA
    210 220 230 240 250
    VNNEIGVVQP MEEIGMICKE HNVPFHTDAA QAIGKIPVDV KKWNVALMSM
    260 270 280 290 300
    SAHKIYGPKG VGALYVRRRP RIRLEPLMNG GGQERGLRSG TGATQQIVGF
    310 320 330 340 350
    GAACELAMKE MEYDEKWIKG LQERLLNGVR EKLDGVVVNG SMDSRYVGNL
    360 370 380 390 400
    NLSFAYVEGE SLLMGLKEVA VSSGSACTSA SLEPSYVLRA LGVDEDMAHT
    410 420 430 440 450
    SIRFGIGRFT TKEEIDKAVE LTVKQVEKLR EMSPLYEMVK EGIDIKNIQW

    SQH
    Length:453
    Mass (Da):50,296
    Last modified:June 1, 1998 - v1
    Checksum:i9BA6987AE45F09AE
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ243393 mRNA Translation: CAB64727.1
    AF229854 mRNA Translation: AAK00758.1
    AB010075 Genomic DNA Translation: BAB10679.1
    AL021684 Genomic DNA Translation: CAA16686.1
    CP002688 Genomic DNA Translation: AED98094.1
    CP002688 Genomic DNA Translation: ANM68565.1
    AY050874 mRNA Translation: AAK92811.1
    AY096358 mRNA Translation: AAM19999.1
    PIRiT05896
    RefSeqiNP_001318886.1, NM_001345717.1 [O49543-1]
    NP_201373.1, NM_125969.3 [O49543-1]
    UniGeneiAt.696

    Genome annotation databases

    EnsemblPlantsiAT5G65720.1; AT5G65720.1; AT5G65720 [O49543-1]
    AT5G65720.3; AT5G65720.3; AT5G65720 [O49543-1]
    GeneIDi836701
    GrameneiAT5G65720.1; AT5G65720.1; AT5G65720 [O49543-1]
    AT5G65720.3; AT5G65720.3; AT5G65720 [O49543-1]
    KEGGiath:AT5G65720

    Keywords - Coding sequence diversityi

    Alternative splicing

    Similar proteinsi

    Entry informationi

    Entry nameiMNIF1_ARATH
    AccessioniPrimary (citable) accession number: O49543
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: June 1, 1998
    Last modified: April 25, 2018
    This is version 149 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health