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Protein

Cysteine desulfurase, mitochondrial

Gene

NIFS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the removal of elemental sulfur from cysteine to produce alanine. Supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters.2 Publications

Catalytic activityi

L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor.1 Publication

Cofactori

pyridoxal 5'-phosphate1 Publication

Enzyme regulationi

Threefold increase in the catalytic activity in the presence of FH (frataxin) (PubMed:22511606). 30-fold increase in the catalytic activity in the presence of SUFE1 (PubMed:16437155).2 Publications

Kineticsi

    1. Vmax=21.2 nmol/min/mg enzyme with cysteine as substrate1 Publication

    pH dependencei

    Optimum pH is 8.5.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei203Pyridoxal phosphateBy similarity1
    Binding sitei231Pyridoxal phosphateBy similarity1
    Binding sitei291Pyridoxal phosphateBy similarity1
    Active sitei377Cysteine persulfide intermediateBy similarity1
    Metal bindingi377Iron-sulfur (2Fe-2S); via persulfide groupBy similarity1

    GO - Molecular functioni

    • ATP binding Source: TAIR
    • cysteine desulfurase activity Source: TAIR
    • iron-sulfur cluster binding Source: UniProtKB-KW
    • pyridoxal phosphate binding Source: InterPro
    • zinc ion binding Source: TAIR

    GO - Biological processi

    • [2Fe-2S] cluster assembly Source: InterPro
    • iron-sulfur cluster assembly Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    Iron, Iron-sulfur, Metal-binding, Pyridoxal phosphate

    Enzyme and pathway databases

    BRENDAi2.8.1.6. 399.
    2.8.1.7. 399.
    ReactomeiR-ATH-1362409. Mitochondrial iron-sulfur cluster biogenesis.
    R-ATH-947581. Molybdenum cofactor biosynthesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cysteine desulfurase, mitochondrial (EC:2.8.1.7)
    Alternative name(s):
    NIFS homolog 11 Publication
    Short name:
    AtNIFS1
    Protein AtMtNifS
    Gene namesi
    Name:NIFS11 Publication
    Synonyms:MtNIFS1, NIFS, NSF11 Publication
    Ordered Locus Names:At5g65720Imported
    ORF Names:F6H11.150Imported, MPA24.7Imported
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    TAIRiAT5G65720.

    Subcellular locationi

    GO - Cellular componenti

    • mitochondrion Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Disruption phenotypei

    Lethal when homozygous.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 34MitochondrionSequence analysisAdd BLAST34
    ChainiPRO_000000129935 – 453Cysteine desulfurase, mitochondrialAdd BLAST419

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei254N6-(pyridoxal phosphate)lysineBy similarity1

    Proteomic databases

    PaxDbiO49543.
    PRIDEiO49543.

    Expressioni

    Gene expression databases

    ExpressionAtlasiO49543. baseline and differential.
    GenevisibleiO49543. AT.

    Interactioni

    Subunit structurei

    Interacts with FH (PubMed:22511606). Interacts with SUFE1 (PubMed:16437155).2 Publications

    Protein-protein interaction databases

    BioGridi21943. 2 interactors.
    STRINGi3702.AT5G65720.1.

    Structurei

    3D structure databases

    ProteinModelPortaliO49543.
    SMRiO49543.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni123 – 124Pyridoxal phosphate bindingBy similarity2
    Regioni251 – 253Pyridoxal phosphate bindingBy similarity3

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiKOG1549. Eukaryota.
    COG1104. LUCA.
    HOGENOMiHOG000017510.
    InParanoidiO49543.
    KOiK04487.
    OMAiEPIQSGG.
    OrthoDBiEOG09360A7M.
    PhylomeDBiO49543.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPiMF_00331. Cys_desulf_IscS. 1 hit.
    InterProiIPR000192. Aminotrans_V_dom.
    IPR010240. Cys_deSase_IscS.
    IPR016454. Cysteine_dSase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00266. Aminotran_5. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005572. NifS. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR02006. IscS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

    Note: A number of isoforms are produced. According to EST sequences.
    Isoform 1 (identifier: O49543-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MASKVISATI RRTLTKPHGT FSRCRYLSTA AAATEVNYED ESIMMKGVRI
    60 70 80 90 100
    SGRPLYLDMQ ATTPIDPRVF DAMNASQIHE YGNPHSRTHL YGWEAENAVE
    110 120 130 140 150
    NARNQVAKLI EASPKEIVFV SGATEANNMA VKGVMHFYKD TKKHVITTQT
    160 170 180 190 200
    EHKCVLDSCR HLQQEGFEVT YLPVKTDGLV DLEMLREAIR PDTGLVSIMA
    210 220 230 240 250
    VNNEIGVVQP MEEIGMICKE HNVPFHTDAA QAIGKIPVDV KKWNVALMSM
    260 270 280 290 300
    SAHKIYGPKG VGALYVRRRP RIRLEPLMNG GGQERGLRSG TGATQQIVGF
    310 320 330 340 350
    GAACELAMKE MEYDEKWIKG LQERLLNGVR EKLDGVVVNG SMDSRYVGNL
    360 370 380 390 400
    NLSFAYVEGE SLLMGLKEVA VSSGSACTSA SLEPSYVLRA LGVDEDMAHT
    410 420 430 440 450
    SIRFGIGRFT TKEEIDKAVE LTVKQVEKLR EMSPLYEMVK EGIDIKNIQW

    SQH
    Length:453
    Mass (Da):50,296
    Last modified:June 1, 1998 - v1
    Checksum:i9BA6987AE45F09AE
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ243393 mRNA. Translation: CAB64727.1.
    AF229854 mRNA. Translation: AAK00758.1.
    AB010075 Genomic DNA. Translation: BAB10679.1.
    AL021684 Genomic DNA. Translation: CAA16686.1.
    CP002688 Genomic DNA. Translation: AED98094.1.
    AY050874 mRNA. Translation: AAK92811.1.
    AY096358 mRNA. Translation: AAM19999.1.
    PIRiT05896.
    RefSeqiNP_201373.1. NM_125969.3. [O49543-1]
    UniGeneiAt.696.

    Genome annotation databases

    EnsemblPlantsiAT5G65720.1; AT5G65720.1; AT5G65720. [O49543-1]
    GeneIDi836701.
    GrameneiAT5G65720.1; AT5G65720.1; AT5G65720.
    KEGGiath:AT5G65720.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ243393 mRNA. Translation: CAB64727.1.
    AF229854 mRNA. Translation: AAK00758.1.
    AB010075 Genomic DNA. Translation: BAB10679.1.
    AL021684 Genomic DNA. Translation: CAA16686.1.
    CP002688 Genomic DNA. Translation: AED98094.1.
    AY050874 mRNA. Translation: AAK92811.1.
    AY096358 mRNA. Translation: AAM19999.1.
    PIRiT05896.
    RefSeqiNP_201373.1. NM_125969.3. [O49543-1]
    UniGeneiAt.696.

    3D structure databases

    ProteinModelPortaliO49543.
    SMRiO49543.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi21943. 2 interactors.
    STRINGi3702.AT5G65720.1.

    Proteomic databases

    PaxDbiO49543.
    PRIDEiO49543.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT5G65720.1; AT5G65720.1; AT5G65720. [O49543-1]
    GeneIDi836701.
    GrameneiAT5G65720.1; AT5G65720.1; AT5G65720.
    KEGGiath:AT5G65720.

    Organism-specific databases

    TAIRiAT5G65720.

    Phylogenomic databases

    eggNOGiKOG1549. Eukaryota.
    COG1104. LUCA.
    HOGENOMiHOG000017510.
    InParanoidiO49543.
    KOiK04487.
    OMAiEPIQSGG.
    OrthoDBiEOG09360A7M.
    PhylomeDBiO49543.

    Enzyme and pathway databases

    BRENDAi2.8.1.6. 399.
    2.8.1.7. 399.
    ReactomeiR-ATH-1362409. Mitochondrial iron-sulfur cluster biogenesis.
    R-ATH-947581. Molybdenum cofactor biosynthesis.

    Miscellaneous databases

    PROiO49543.

    Gene expression databases

    ExpressionAtlasiO49543. baseline and differential.
    GenevisibleiO49543. AT.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPiMF_00331. Cys_desulf_IscS. 1 hit.
    InterProiIPR000192. Aminotrans_V_dom.
    IPR010240. Cys_deSase_IscS.
    IPR016454. Cysteine_dSase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00266. Aminotran_5. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005572. NifS. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR02006. IscS. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiMNIF1_ARATH
    AccessioniPrimary (citable) accession number: O49543
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: June 1, 1998
    Last modified: November 30, 2016
    This is version 139 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.