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Protein

Phospholipase A1-IIgamma

Gene

DSEL

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acylhydrolase that catalyzes the hydrolysis of 1,3-diacylglycerol (1,3-DAG) and 1-monoacylglycerol (1-MAG) at the sn-1 position. High activity toward 1,3-DAG and 1-MAG, but low activity toward 1,2-diacylglycerol (1,2-DAG) and 1-lysophosphatidylcholine (1-LPC), and no activity toward phosphatidylcholine (PC), monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), triacylglycerol (TAG) and 2-monoacylglycerol (2-MAG). May be involved in the negative regulation of seedling establishment by inhibiting the breakdown, beta-oxidation and mobilization of seed storage oils.1 Publication

pH dependencei

Optimum pH is 5-6 with 1,3-DAG as substrate and at 30 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei236Acyl-ester intermediateBy similarity1
Active sitei236Charge relay systemBy similarity1
Active sitei302Charge relay systemBy similarity1
Active sitei339Charge relay systemBy similarity1

GO - Molecular functioni

  • acylglycerol lipase activity Source: TAIR
  • phosphatidylcholine 1-acylhydrolase activity Source: UniProtKB

GO - Biological processi

  • diacylglycerol catabolic process Source: TAIR
  • lipid storage Source: TAIR
  • monoacylglycerol catabolic process Source: TAIR
  • negative regulation of seed germination Source: UniProtKB

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:AT4G18550-MONOMER

Protein family/group databases

ESTHERiarath-At4g18550 Plant_phospholipase

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase A1-IIgamma (EC:3.1.1.-)
Alternative name(s):
DAD1-like seedling establishment-related lipase
Short name:
AtDSEL
Short name:
Phospholipase DSEL
Gene namesi
Name:DSEL
Ordered Locus Names:At4g18550
ORF Names:F28J12.210
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G18550
TAIRilocus:2124529 AT4G18550

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mildly fast-growing seedlings regardless of the presence of an exogenous carbon source, accompanied by a better beta-oxidation and mobilization of seed storage oils.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004093611 – 419Phospholipase A1-IIgammaAdd BLAST419

Proteomic databases

PaxDbiO49523
PRIDEiO49523

Expressioni

Tissue specificityi

Expressed in seedlings, stems and siliques, and, to a lower extent, in flowers.1 Publication

Gene expression databases

ExpressionAtlasiO49523 baseline and differential
GenevisibleiO49523 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G18550.1

Structurei

Secondary structure

1419
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni20 – 22Combined sources3
Helixi23 – 26Combined sources4
Turni27 – 34Combined sources8
Turni36 – 39Combined sources4
Helixi41 – 59Combined sources19
Turni68 – 71Combined sources4
Beta strandi72 – 75Combined sources4
Helixi80 – 83Combined sources4
Beta strandi95 – 103Combined sources9
Turni111 – 113Combined sources3
Beta strandi128 – 136Combined sources9
Helixi138 – 144Combined sources7
Beta strandi146 – 153Combined sources8
Beta strandi170 – 172Combined sources3
Helixi174 – 177Combined sources4
Helixi179 – 181Combined sources3
Beta strandi185 – 187Combined sources3
Helixi188 – 195Combined sources8
Turni202 – 204Combined sources3
Helixi208 – 222Combined sources15
Turni223 – 225Combined sources3
Beta strandi228 – 235Combined sources8
Helixi237 – 251Combined sources15
Beta strandi267 – 273Combined sources7
Helixi280 – 287Combined sources8
Beta strandi292 – 299Combined sources8
Helixi304 – 306Combined sources3
Beta strandi316 – 321Combined sources6
Helixi323 – 325Combined sources3
Helixi335 – 339Combined sources5
Helixi341 – 350Combined sources10
Helixi368 – 373Combined sources6
Helixi380 – 382Combined sources3
Helixi392 – 395Combined sources4
Beta strandi396 – 398Combined sources3
Beta strandi404 – 406Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YIJX-ray2.00A/B1-419[»]
ProteinModelPortaliO49523
SMRiO49523
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1 – 21Sequence analysisAdd BLAST21
Coiled coili207 – 227Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Lipase family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4569 Eukaryota
COG3675 LUCA
HOGENOMiHOG000238127
InParanoidiO49523
OMAiCLFNRRD
OrthoDBiEOG09360AAB
PhylomeDBiO49523

Family and domain databases

InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR002921 Fungal_lipase-like
IPR033556 PLA
PANTHERiPTHR31828 PTHR31828, 1 hit
PfamiView protein in Pfam
PF01764 Lipase_3, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit

Sequencei

Sequence statusi: Complete.

O49523-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRKKKEEEE EKLIVTREFA KRWRDLSGQN HWKGMLQPLD QDLREYIIHY
60 70 80 90 100
GEMAQAGYDT FNINTESQFA GASIYSRKDF FAKVGLEIAH PYTKYKVTKF
110 120 130 140 150
IYATSDIHVP ESFLLFPISR EGWSKESNWM GYVAVTDDQG TALLGRRDIV
160 170 180 190 200
VSWRGSVQPL EWVEDFEFGL VNAIKIFGER NDQVQIHQGW YSIYMSQDER
210 220 230 240 250
SPFTKTNARD QVLREVGRLL EKYKDEEVSI TICGHSLGAA LATLSATDIV
260 270 280 290 300
ANGYNRPKSR PDKSCPVTAF VFASPRVGDS DFRKLFSGLE DIRVLRTRNL
310 320 330 340 350
PDVIPIYPPI GYSEVGDEFP IDTRKSPYMK SPGNLATFHC LEGYLHGVAG
360 370 380 390 400
TQGTNKADLF RLDVERAIGL VNKSVDGLKD ECMVPGKWRV LKNKGMAQQD
410
DGSWELVDHE IDDNEDLDF
Length:419
Mass (Da):47,792
Last modified:June 1, 1998 - v1
Checksum:i3B2B13089146482C
GO

Sequence cautioni

The sequence BAC42692 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL021710 Genomic DNA Translation: CAA16735.1
AL161548 Genomic DNA Translation: CAB78857.1
CP002687 Genomic DNA Translation: AEE84060.1
BT030056 mRNA Translation: ABN04794.1
AK118061 mRNA Translation: BAC42692.1 Different initiation.
PIRiT04551
RefSeqiNP_193590.1, NM_117969.3
UniGeneiAt.32892

Genome annotation databases

EnsemblPlantsiAT4G18550.1; AT4G18550.1; AT4G18550
AT4G18550.2; AT4G18550.2; AT4G18550
GeneIDi827587
GrameneiAT4G18550.1; AT4G18550.1; AT4G18550
AT4G18550.2; AT4G18550.2; AT4G18550
KEGGiath:AT4G18550

Similar proteinsi

Entry informationi

Entry nameiDSEL_ARATH
AccessioniPrimary (citable) accession number: O49523
Secondary accession number(s): Q8GXS9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: June 1, 1998
Last modified: April 25, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome