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O49506

- GLO5_ARATH

UniProt

O49506 - GLO5_ARATH

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Protein

Peroxisomal (S)-2-hydroxy-acid oxidase GLO5

Gene
GLO5, GOX3, At4g18360, F28J12.20
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2.

Cofactori

FMN By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei24 – 241Substrate Reviewed prediction
Binding sitei106 – 1061FMN By similarity
Binding sitei127 – 1271FMN By similarity
Binding sitei129 – 1291Substrate By similarity
Binding sitei155 – 1551FMN By similarity
Binding sitei164 – 1641Substrate By similarity
Binding sitei230 – 2301FMN By similarity
Active sitei254 – 2541Proton acceptor By similarity
Binding sitei257 – 2571Substrate Reviewed prediction

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi285 – 30925FMN By similarityAdd
BLAST

GO - Molecular functioni

  1. FMN binding Source: InterPro
  2. long-chain-(S)-2-hydroxy-long-chain-acid oxidase activity Source: UniProtKB-EC
  3. medium-chain-(S)-2-hydroxy-acid oxidase activity Source: UniProtKB-EC
  4. very-long-chain-(S)-2-hydroxy-acid oxidase activity Source: UniProtKB-EC

GO - Biological processi

  1. defense response signaling pathway, resistance gene-independent Source: TAIR
  2. defense response to bacterium Source: TAIR
  3. hydrogen peroxide biosynthetic process Source: TAIR
  4. oxidative photosynthetic carbon pathway Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolate pathway, Photorespiration

Keywords - Ligandi

Flavoprotein, FMN

Enzyme and pathway databases

BioCyciARA:AT4G18360-MONOMER.
RETL1328306-WGS:GSTH-433-MONOMER.
UniPathwayiUPA00951; UER00912.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal (S)-2-hydroxy-acid oxidase GLO5 (EC:1.1.3.15)
Alternative name(s):
Glycolate oxidase 3
Short name:
AtGLO5
Short name:
GOX 3
Short chain alpha-hydroxy acid oxidase GLO5
Gene namesi
Name:GLO5
Synonyms:GOX3
Ordered Locus Names:At4g18360
ORF Names:F28J12.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G18360.

Subcellular locationi

Peroxisome 1 Publication

GO - Cellular componenti

  1. peroxisome Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 368368Peroxisomal (S)-2-hydroxy-acid oxidase GLO5PRO_0000403408Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO49506.
PRIDEiO49506.

Expressioni

Gene expression databases

GenevestigatoriO49506.

Interactioni

Subunit structurei

Homotetramer or homooctamer By similarity.

Protein-protein interaction databases

STRINGi3702.AT4G18360.1-P.

Structurei

3D structure databases

ProteinModelPortaliO49506.
SMRiO49506. Positions 1-359.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 359359FMN hydroxy acid dehydrogenaseAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi366 – 3683Microbody targeting signal Reviewed prediction

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1304.
HOGENOMiHOG000217463.
InParanoidiO49506.
KOiK11517.
OMAiARIAVEY.
PhylomeDBiO49506.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR012133. Alpha-hydoxy_acid_DH_FMN.
IPR000262. FMN-dep_DH.
IPR008259. FMN_hydac_DH_AS.
[Graphical view]
PfamiPF01070. FMN_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITEiPS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O49506-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEITNVMEYE KIAKEKLPKM VYDYYASGAE DQWTLQENRN AFSRILFRPR    50
ILIDVSKIDV STTVLGFNIS MPIMIAPTAM QKMAHPDGEL ATARATSAAG 100
TIMTLSSWAT CSVEEVASTG PGIRFFQLYV YKDRNVVIQL VKRAEEAGFK 150
AIALTVDTPR LGRRESDIKN RFALPRGLTL KNFEGLDLGK IDKTNDSGLA 200
SYVAGQVDQS LSWKDIKWLQ SITSLPILVK GVITAEDARI AVEYGAAGII 250
VSNHGARQLD YVPATIVALE EVVKAVEGRI PVFLDGGVRR GTDVFKALAL 300
GASGVFVGRP SLFSLAADGE AGVRKMLQML RDEFELTMAL SGCRSLREIS 350
RTHIKTDWDT PHYLSAKL 368
Length:368
Mass (Da):40,482
Last modified:June 1, 1998 - v1
Checksum:i3B3B33BB0562F5AB
GO
Isoform 2 (identifier: O49506-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     308-314: GRPSLFS → SSFIIYT
     315-368: Missing.

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:314
Mass (Da):34,404
Checksum:i94F9E8721DFCC680
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei308 – 3147GRPSLFS → SSFIIYT in isoform 2. VSP_040388
Alternative sequencei315 – 36854Missing in isoform 2. VSP_040389Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL021710 Genomic DNA. Translation: CAA16716.1.
AL161548 Genomic DNA. Translation: CAB78838.1.
CP002687 Genomic DNA. Translation: AEE84031.1.
CP002687 Genomic DNA. Translation: AEE84032.1.
BT001945 mRNA. Translation: AAN71944.1.
PIRiG85206.
T04532.
RefSeqiNP_001078406.1. NM_001084937.1. [O49506-2]
NP_193570.1. NM_117946.2. [O49506-1]
UniGeneiAt.24604.

Genome annotation databases

EnsemblPlantsiAT4G18360.1; AT4G18360.1; AT4G18360. [O49506-1]
GeneIDi827563.
KEGGiath:AT4G18360.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL021710 Genomic DNA. Translation: CAA16716.1 .
AL161548 Genomic DNA. Translation: CAB78838.1 .
CP002687 Genomic DNA. Translation: AEE84031.1 .
CP002687 Genomic DNA. Translation: AEE84032.1 .
BT001945 mRNA. Translation: AAN71944.1 .
PIRi G85206.
T04532.
RefSeqi NP_001078406.1. NM_001084937.1. [O49506-2 ]
NP_193570.1. NM_117946.2. [O49506-1 ]
UniGenei At.24604.

3D structure databases

ProteinModelPortali O49506.
SMRi O49506. Positions 1-359.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT4G18360.1-P.

Proteomic databases

PaxDbi O49506.
PRIDEi O49506.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G18360.1 ; AT4G18360.1 ; AT4G18360 . [O49506-1 ]
GeneIDi 827563.
KEGGi ath:AT4G18360.

Organism-specific databases

TAIRi AT4G18360.

Phylogenomic databases

eggNOGi COG1304.
HOGENOMi HOG000217463.
InParanoidi O49506.
KOi K11517.
OMAi ARIAVEY.
PhylomeDBi O49506.

Enzyme and pathway databases

UniPathwayi UPA00951 ; UER00912 .
BioCyci ARA:AT4G18360-MONOMER.
RETL1328306-WGS:GSTH-433-MONOMER.

Gene expression databases

Genevestigatori O49506.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR012133. Alpha-hydoxy_acid_DH_FMN.
IPR000262. FMN-dep_DH.
IPR008259. FMN_hydac_DH_AS.
[Graphical view ]
Pfami PF01070. FMN_dh. 1 hit.
[Graphical view ]
PIRSFi PIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITEi PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Proteome analysis of Arabidopsis leaf peroxisomes reveals novel targeting peptides, metabolic pathways, and defense mechanisms."
    Reumann S., Babujee L., Ma C., Wienkoop S., Siemsen T., Antonicelli G.E., Rasche N., Lueder F., Weckwerth W., Jahn O.
    Plant Cell 19:3170-3193(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. "Inducible antisense suppression of glycolate oxidase reveals its strong regulation over photosynthesis in rice."
    Xu H.-W., Zhang J., Zeng J., Jiang L., Liu E., Peng C., He Z.-H., Peng X.-X.
    J. Exp. Bot. 60:1799-1809(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGLO5_ARATH
AccessioniPrimary (citable) accession number: O49506
Secondary accession number(s): A8MRC3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: June 1, 1998
Last modified: July 9, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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