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Protein

Peroxisomal (S)-2-hydroxy-acid oxidase GLO5

Gene

GLO5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2.

Cofactori

FMNPROSITE-ProRule annotation

Pathwayi: photorespiration

This protein is involved in step 2 of the subpathway that synthesizes glycine from 2-phosphoglycolate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Peroxisomal (S)-2-hydroxy-acid oxidase GLO5 (GLO5), Peroxisomal (S)-2-hydroxy-acid oxidase GLO2 (GLO2), Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 (GLO3), Peroxisomal (S)-2-hydroxy-acid oxidase GLO1 (GLO1), Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 (GLO4)
  3. no protein annotated in this organism
This subpathway is part of the pathway photorespiration, which is itself part of Photosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycine from 2-phosphoglycolate, the pathway photorespiration and in Photosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei24SubstratePROSITE-ProRule annotation1
Binding sitei106FMNPROSITE-ProRule annotation1
Binding sitei127FMNPROSITE-ProRule annotation1
Binding sitei129SubstratePROSITE-ProRule annotation1
Binding sitei155FMNPROSITE-ProRule annotation1
Binding sitei164SubstratePROSITE-ProRule annotation1
Binding sitei230FMNPROSITE-ProRule annotation1
Active sitei254Proton acceptorPROSITE-ProRule annotation1
Binding sitei257SubstratePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi285 – 309FMNPROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

  • defense response signaling pathway, resistance gene-independent Source: TAIR
  • defense response to bacterium Source: TAIR
  • hydrogen peroxide biosynthetic process Source: TAIR
  • oxidative photosynthetic carbon pathway Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolate pathway, Photorespiration

Keywords - Ligandi

Flavoprotein, FMN

Enzyme and pathway databases

BioCyciARA:AT4G18360-MONOMER.
ReactomeiR-ATH-389661. Glyoxylate metabolism and glycine degradation.
R-ATH-390918. Peroxisomal lipid metabolism.
UniPathwayiUPA00951; UER00912.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal (S)-2-hydroxy-acid oxidase GLO5 (EC:1.1.3.15)
Alternative name(s):
Glycolate oxidase 3
Short name:
AtGLO5
Short name:
GOX 3
Short chain alpha-hydroxy acid oxidase GLO5
Gene namesi
Name:GLO5
Synonyms:GOX3
Ordered Locus Names:At4g18360
ORF Names:F28J12.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G18360.

Subcellular locationi

GO - Cellular componenti

  • peroxisome Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004034081 – 368Peroxisomal (S)-2-hydroxy-acid oxidase GLO5Add BLAST368

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO49506.
PRIDEiO49506.

Expressioni

Gene expression databases

GenevisibleiO49506. AT.

Interactioni

Subunit structurei

Homotetramer or homooctamer.By similarity

Protein-protein interaction databases

STRINGi3702.AT4G18360.1.

Structurei

3D structure databases

ProteinModelPortaliO49506.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 359FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd BLAST359

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi366 – 368Microbody targeting signalSequence analysis3

Sequence similaritiesi

Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.PROSITE-ProRule annotation
Contains 1 FMN hydroxy acid dehydrogenase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0538. Eukaryota.
COG1304. LUCA.
HOGENOMiHOG000217463.
InParanoidiO49506.
KOiK11517.
OMAiETMRANR.
OrthoDBiEOG09360EDE.
PhylomeDBiO49506.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR012133. Alpha-hydoxy_acid_DH_FMN.
IPR000262. FMN-dep_DH.
IPR008259. FMN_hydac_DH_AS.
[Graphical view]
PfamiPF01070. FMN_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITEiPS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O49506-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEITNVMEYE KIAKEKLPKM VYDYYASGAE DQWTLQENRN AFSRILFRPR
60 70 80 90 100
ILIDVSKIDV STTVLGFNIS MPIMIAPTAM QKMAHPDGEL ATARATSAAG
110 120 130 140 150
TIMTLSSWAT CSVEEVASTG PGIRFFQLYV YKDRNVVIQL VKRAEEAGFK
160 170 180 190 200
AIALTVDTPR LGRRESDIKN RFALPRGLTL KNFEGLDLGK IDKTNDSGLA
210 220 230 240 250
SYVAGQVDQS LSWKDIKWLQ SITSLPILVK GVITAEDARI AVEYGAAGII
260 270 280 290 300
VSNHGARQLD YVPATIVALE EVVKAVEGRI PVFLDGGVRR GTDVFKALAL
310 320 330 340 350
GASGVFVGRP SLFSLAADGE AGVRKMLQML RDEFELTMAL SGCRSLREIS
360
RTHIKTDWDT PHYLSAKL
Length:368
Mass (Da):40,482
Last modified:June 1, 1998 - v1
Checksum:i3B3B33BB0562F5AB
GO
Isoform 2 (identifier: O49506-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     308-314: GRPSLFS → SSFIIYT
     315-368: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:314
Mass (Da):34,404
Checksum:i94F9E8721DFCC680
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_040388308 – 314GRPSLFS → SSFIIYT in isoform 2. Curated7
Alternative sequenceiVSP_040389315 – 368Missing in isoform 2. CuratedAdd BLAST54

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL021710 Genomic DNA. Translation: CAA16716.1.
AL161548 Genomic DNA. Translation: CAB78838.1.
CP002687 Genomic DNA. Translation: AEE84031.1.
CP002687 Genomic DNA. Translation: AEE84032.1.
BT001945 mRNA. Translation: AAN71944.1.
PIRiG85206.
T04532.
RefSeqiNP_001078406.1. NM_001084937.1. [O49506-2]
NP_193570.1. NM_117946.3. [O49506-1]
UniGeneiAt.24604.

Genome annotation databases

EnsemblPlantsiAT4G18360.1; AT4G18360.1; AT4G18360. [O49506-1]
GeneIDi827563.
GrameneiAT4G18360.1; AT4G18360.1; AT4G18360.
KEGGiath:AT4G18360.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL021710 Genomic DNA. Translation: CAA16716.1.
AL161548 Genomic DNA. Translation: CAB78838.1.
CP002687 Genomic DNA. Translation: AEE84031.1.
CP002687 Genomic DNA. Translation: AEE84032.1.
BT001945 mRNA. Translation: AAN71944.1.
PIRiG85206.
T04532.
RefSeqiNP_001078406.1. NM_001084937.1. [O49506-2]
NP_193570.1. NM_117946.3. [O49506-1]
UniGeneiAt.24604.

3D structure databases

ProteinModelPortaliO49506.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G18360.1.

Proteomic databases

PaxDbiO49506.
PRIDEiO49506.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G18360.1; AT4G18360.1; AT4G18360. [O49506-1]
GeneIDi827563.
GrameneiAT4G18360.1; AT4G18360.1; AT4G18360.
KEGGiath:AT4G18360.

Organism-specific databases

TAIRiAT4G18360.

Phylogenomic databases

eggNOGiKOG0538. Eukaryota.
COG1304. LUCA.
HOGENOMiHOG000217463.
InParanoidiO49506.
KOiK11517.
OMAiETMRANR.
OrthoDBiEOG09360EDE.
PhylomeDBiO49506.

Enzyme and pathway databases

UniPathwayiUPA00951; UER00912.
BioCyciARA:AT4G18360-MONOMER.
ReactomeiR-ATH-389661. Glyoxylate metabolism and glycine degradation.
R-ATH-390918. Peroxisomal lipid metabolism.

Miscellaneous databases

PROiO49506.

Gene expression databases

GenevisibleiO49506. AT.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR012133. Alpha-hydoxy_acid_DH_FMN.
IPR000262. FMN-dep_DH.
IPR008259. FMN_hydac_DH_AS.
[Graphical view]
PfamiPF01070. FMN_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITEiPS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLO5_ARATH
AccessioniPrimary (citable) accession number: O49506
Secondary accession number(s): A8MRC3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: June 1, 1998
Last modified: November 30, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.