ID   NCED2_ARATH             Reviewed;         583 AA.
AC   O49505;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   16-JUN-2009, entry version 46.
DE   RecName: Full=9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic;
DE            Short=AtNCED2;
DE            EC=1.13.11.51;
DE   Flags: Precursor;
GN   Name=NCED2; OrderedLocusNames=At4g18350; ORFNames=F28J12.10;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=20083488; PubMed=10617198; DOI=10.1038/47134;
RA   Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G.,
RA   Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N.,
RA   Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M.,
RA   Weichselgartner M., de Simone V., Obermaier B., Mache R., Mueller M.,
RA   Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T.,
RA   Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I.,
RA   Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P.,
RA   Langham S.-A., McCullagh B., Bilham L., Robben J.,
RA   van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F.,
RA   Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E.,
RA   Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA   Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W.,
RA   Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P.,
RA   Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H.,
RA   De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R.,
RA   van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S.,
RA   Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R.,
RA   Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S.,
RA   Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H.,
RA   Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S.,
RA   Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A.,
RA   Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA   Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R.,
RA   Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S.,
RA   Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E.,
RA   Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A.,
RA   Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T.,
RA   Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C.,
RA   Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S.,
RA   Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K.,
RA   Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L.,
RA   Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J.,
RA   Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J.,
RA   Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D.,
RA   Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA   Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA   Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D.,
RA   Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C.,
RA   Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C.,
RA   Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R.,
RA   Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S.,
RA   Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A.,
RA   Chen E., Marra M.A., Martienssen R., McCombie W.R.;
RT   "Sequence and analysis of chromosome 4 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 402:769-777(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C.,
RA   Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION.
RX   PubMed=11532178; DOI=10.1046/j.1365-313x.2001.01096.x;
RA   Iuchi S., Kobayashi M., Taji T., Naramoto M., Seki M., Kato T.,
RA   Tabata S., Kakubari Y., Yamaguchi-Shinozaki K., Shinozaki K.;
RT   "Regulation of drought tolerance by gene manipulation of 9-cis-
RT   epoxycarotenoid dioxygenase, a key enzyme in abscisic acid
RT   biosynthesis in Arabidopsis.";
RL   Plant J. 27:325-333(2001).
RN   [4]
RP   SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION BY DROUGHT
RP   STRESS.
RX   PubMed=12834401; DOI=10.1046/j.1365-313X.2003.01786.x;
RA   Tan B.-C., Joseph L.M., Deng W.-T., Liu L., Li Q.-B., Cline K.,
RA   McCarty D.R.;
RT   "Molecular characterization of the Arabidopsis 9-cis epoxycarotenoid
RT   dioxygenase gene family.";
RL   Plant J. 35:44-56(2003).
RN   [5]
RP   TISSUE SPECIFICITY.
RX   PubMed=16412079; DOI=10.1111/j.1365-313X.2005.02622.x;
RA   Lefebvre V., North H., Frey A., Sotta B., Seo M., Okamoto M.,
RA   Nambara E., Marion-Poll A.;
RT   "Functional analysis of Arabidopsis NCED6 and NCED9 genes indicates
RT   that ABA synthesized in the endosperm is involved in the induction of
RT   seed dormancy.";
RL   Plant J. 45:309-319(2006).
CC   -!- FUNCTION: Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage
CC       activity. Catalyzes the first step of abscisic-acid biosynthesis
CC       from carotenoids.
CC   -!- CATALYTIC ACTIVITY: A 9-cis-epoxycarotenoid + O(2) = 2-cis,4-
CC       trans-xanthoxin + a 12'-apo-carotenal.
CC   -!- CATALYTIC ACTIVITY: 9-cis-violaxanthin + O(2) = 2-cis,4-trans-
CC       xanthoxin + (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-dihydro-12'-apo-
CC       beta-caroten-12'-al.
CC   -!- CATALYTIC ACTIVITY: 9'-cis-neoxanthin + O(2) = 2-cis,4-trans-
CC       xanthoxin + (3S,5R,6R)-5,6-dihydroxy-6,7-didehydro-5,6-dihydro-
CC       12'-apo-beta-caroten-12'-al.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma. Note=Partially
CC       bound to the thylakoid.
CC   -!- TISSUE SPECIFICITY: Very low in all tissues. Expressed at the
CC       point of organ attachment and the abscission zones in the plant.
CC   -!- INDUCTION: Low induction by drought stress.
CC   -!- SIMILARITY: Belongs to the carotenoid oxygenase family.
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DR   EMBL; AL021710; CAA16715.1; -; Genomic_DNA.
DR   EMBL; AL161548; CAB78837.1; -; Genomic_DNA.
DR   EMBL; BT030365; ABO38778.1; -; mRNA.
DR   IPI; IPI00523545; -.
DR   PIR; T04531; T04531.
DR   RefSeq; NP_193569.1; -.
DR   UniGene; At.32916; -.
DR   GeneID; 827562; -.
DR   GenomeReviews; CT486007_GR; AT4G18350.
DR   KEGG; ath:AT4G18350; -.
DR   NMPDR; fig|3702.1.peg.19634; -.
DR   TAIR; At4g18350; -.
DR   BRENDA; 1.13.11.51; 302.
DR   ArrayExpress; O49505; -.
DR   GO; GO:0009570; C:chloroplast stroma; IDA:TAIR.
DR   GO; GO:0009535; C:chloroplast thylakoid membrane; IDA:TAIR.
DR   GO; GO:0045549; F:9-cis-epoxycarotenoid dioxygenase activity; IDA:TAIR.
DR   GO; GO:0009688; P:abscisic acid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR004294; Carotenoid_Oase.
DR   PANTHER; PTHR10543; Carotenoid_Oase; 1.
DR   Pfam; PF03055; RPE65; 1.
PE   2: Evidence at transcript level;
KW   Abscisic acid biosynthesis; Chloroplast; Complete proteome;
KW   Dioxygenase; Oxidoreductase; Plastid; Transit peptide.
FT   TRANSIT       1      ?       Chloroplast (Potential).
FT   CHAIN         ?    583       9-cis-epoxycarotenoid dioxygenase NCED2,
FT                                chloroplastic.
FT                                /FTId=PRO_0000285990.
SQ   SEQUENCE   583 AA;  65067 MW;  A138F93542E50852 CRC64;
     MVSLLTMPMS GGIKTWPQAQ IDLGFRPIKR QPKVIKCTVQ IDVTELTKKR QLFTPRTTAT
     PPQHNPLRLN IFQKAAAIAI DAAERALISH EQDSPLPKTA DPRVQIAGNY SPVPESSVRR
     NLTVEGTIPD CIDGVYIRNG ANPMFEPTAG HHLFDGDGMV HAVKITNGSA SYACRFTKTE
     RLVQEKRLGR PVFPKAIGEL HGHSGIARLM LFYARGLCGL INNQNGVGVA NAGLVYFNNR
     LLAMSEDDLP YQLKITQTGD LQTVGRYDFD GQLKSAMIAH PKLDPVTKEL HALSYDVVKK
     PYLKYFRFSP DGVKSPELEI PLETPTMIHD FAITENFVVI PDQQVVFKLG EMISGKSPVV
     FDGEKVSRLG IMPKDATEAS QIIWVNSPET FCFHLWNAWE SPETEEIVVI GSCMSPADSI
     FNERDESLRS VLSEIRINLR TRKTTRRSLL VNEDVNLEIG MVNRNRLGRK TRFAFLAIAY
     PWPKVSGFAK VDLCTGEMKK YIYGGEKYGG EPFFLPGNSG NGEENEDDGY IFCHVHDEET
     KTSELQIINA VNLKLEATIK LPSRVPYGFH GTFVDSNELV DQL
//