Reviewed, UniProtKB/Swiss-Prot O49484 (ASK11_ARATH)

Names and origin

Protein names

Recommended name:
    SKP1-like protein 11
      Short name=AtSK11

Gene names

Name:	ASK11
Ordered Locus Names:	At4g34210
ORF Names:	F10M10.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Together with CUL1, RBX1 and a F-box protein, it forms a SCF E3 ubiquitin ligase complex. The functional specificity of this complex depends of the type of F-box protein. In the SCF complex, it serves as an adapter that links the F-box protein to CUL1 By similarity. Plays a role during early flowers reproductive development.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Part of a SCF (SKP1-cullin-F-box) protein ligase complex By similarity. Interacts with ADO3/FKF1, COI1/FBL2, EBF1/FBL6, PP2A13, PP2B10, UFO, SKIP2, SKIP15, SKIP16, SKIP32, At1g55000, At1g67340, At1g78100, At3g04660, At3g16740, At3g61590, At4g38940 and At5g49610.
Subcellular location	Nucleus By similarity.
Tissue specificity	Expressed in young seedlings, cotyledons, roots, leaves, floral stems, inflorescences, pollen, and siliques, with a slightly higher level in inflorescence than in other tissues.
Developmental stage	Expressed in the inflorescence meristem (IM) and young buds, particularly in stamen. Also detected in pollen grains.
Sequence similarities	Belongs to the SKP1 family.
Sequence caution	The sequence ABK28664.1 differs from that shown. Reason: Erroneous termination at position 153. Translated as stop.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ligase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.3 Ref.5 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ADO1	Q94BT6	2	EBI-401185,EBI-300691
ADO3	Q9C9W9	2	EBI-401185,EBI-401228
EBF1	Q9SKK0	2	EBI-401185,EBI-401198
FBX3	O49279	2	EBI-401185,EBI-591174
PP2A13	Q9LEX0	2	EBI-401185,EBI-604261
UFO	Q39090	2	EBI-401185,EBI-590758

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 152	152	SKP1-like protein 11		PRO_0000375252
Regions
	Region	94 – 152	59	Interaction with the F-box domain of F-box proteins By similarity

Sequences

	Sequence		Length	Mass (Da)
	O49484-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 95D23CC17C1523B0	FASTA	152	17,288
	10 20 30 40 50 60 MSSKMIVLMS SDGQSFEVEE AVAIQSQTIA HMVEDDCVAD GIPLANVESK ILVKVIEYCK 70 80 90 100 110 120 KHHVDEANPI SEEDLNNWDE KFMDLEQSTI FELILAANYL NIKSLLDLTC QTVADMIKGK 130 140 150 TPEEIRSTFN IENDFTPEEE EAVRKENQWA FE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations." Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P. Plant Biotechnol. J. 4:317-324(2006) [PubMed: 17147637] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"The F-box subunit of the SCF E3 complex is encoded by a diverse superfamily of genes in Arabidopsis." Gagne J.M., Downes B.P., Shiu S.-H., Durski A.M., Vierstra R.D. Proc. Natl. Acad. Sci. U.S.A. 99:11519-11524(2002) [PubMed: 12169662] [Abstract] Cited for: INTERACTION WITH UFO; PP2A13; AT1G67340; AT4G38940; SKIP15; AT3G16740; AT3G04660; AT1G78100; AT1G55000; SKIP16; SKIP2; SKIP32 AND EBF1.
[4]	"Members of the Arabidopsis-SKP1-like gene family exhibit a variety of expression patterns and may play diverse roles in Arabidopsis." Zhao D., Ni W., Feng B., Han T., Petrasek M.G., Ma H. Plant Physiol. 133:203-217(2003) [PubMed: 12970487] [Abstract] Cited for: FUNCTION, GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[5]	"Expression and interaction analysis of Arabidopsis Skp1-related genes." Takahashi N., Kuroda H., Kuromori T., Hirayama T., Seki M., Shinozaki K., Shimada H., Matsui M. Plant Cell Physiol. 45:83-91(2004) [PubMed: 14749489] [Abstract] Cited for: TISSUE SPECIFICITY, INTERACTION WITH EBF1/FBL6; COI1/FBL2; ADO3/FKF1; PP2B10; AT3G61590 AND AT5G49610.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL021961 Genomic DNA. Translation: CAA17551.1. AL161585 Genomic DNA. Translation: CAB80138.1. DQ446893 mRNA. Translation: ABE66110.1. DQ653243 mRNA. Translation: ABK28664.1.
IPI	IPI00541308.
PIR	T05415.
RefSeq	NP_567959.1.
UniGene	At.54598
3D structure databases
HSSP	HSSP built from PDB template 1FQV based on UniProtKB P34991.
SMR	O49484. Positions 6-152.
ModBase	Search...
Protein-protein interaction databases
IntAct	O49484. 34 interactions.
Genome annotation databases
GeneID	829569.
KEGG	ath:AT4G34210.
NMPDR	fig|3702.1.peg.21481.
Organism-specific databases
TAIR	At4g34210.
Phylogenomic databases
OMA	O49484. IRRAFNI.
Gene expression databases
ArrayExpress	O49484.
Family and domain databases
InterPro	IPR011333. BTB/POZ_fold. IPR016897. E3_ubiquit_lig_SCF_Skp. IPR001232. Skp1_comp. IPR016072. Skp1_comp_dimer. IPR016073. Skp1_comp_POZ. [Graphical view]
Gene3D	G3DSA:3.30.710.10. BTB/POZ_fold. 1 hit.
PANTHER	PTHR11165. Skp1. 1 hit.
Pfam	PF01466. Skp1. 1 hit. PF03931. Skp1_POZ. 1 hit. [Graphical view]
PIRSF	PIRSF028729. E3_ubiquit_lig_SCF_Skp. 1 hit.
SMART	SM00512. Skp1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASK11_ARATH

Accession

Primary (citable) accession number: O49484
Secondary accession number(s): A0MFB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	June 1, 1998
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)