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Protein

Allantoate deiminase

Gene

AAH

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the catabolism of purine nucleotides. Can use allantoate as substrate, but not Nalpha-carbamoyl-L-Asp, Nalpha-carbamoyl-L-Ala or Nalpha-carbamoyl-Gly. The sequential activity of AAH, UGLYAH and UAH allows a complete purine breakdown without the intermediate generation of urea.4 Publications

Catalytic activityi

Allantoate + H2O = (S)-ureidoglycine + NH3 + CO2.2 Publications

Cofactori

Mn2+1 Publication

Enzyme regulationi

Inhibited by borate, fluoride, L-Asn and L-Asp, but not by phenylphosphorodiamidate.1 Publication

Kineticsi

kcat is 27.2 sec(-1) for allantoate.1 Publication

Manual assertion based on experiment ini

      Sites

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Metal bindingi169Manganese 1By similarity1
      Metal bindingi178Manganese 1By similarity1
      Metal bindingi178Manganese 2By similarity1
      Metal bindingi215Manganese 2By similarity1
      Metal bindingi281Manganese 1By similarity1
      Metal bindingi499Manganese 2By similarity1

      GO - Molecular functioni

      GO - Biological processi

      • purine nucleobase catabolic process Source: TAIR
      • ureide catabolic process Source: TAIR
      Complete GO annotation...

      Keywords - Molecular functioni

      Hydrolase

      Keywords - Biological processi

      Purine metabolism

      Keywords - Ligandi

      Manganese, Metal-binding

      Enzyme and pathway databases

      BioCyciARA:AT4G20070-MONOMER.
      MetaCyc:AT4G20070-MONOMER.
      BRENDAi3.5.3.9. 399.

      Protein family/group databases

      MEROPSiM20.A07.

      Names & Taxonomyi

      Protein namesi
      Recommended name:
      Allantoate deiminase (EC:3.5.3.9)
      Alternative name(s):
      Allantoate amidohydrolase
      Short name:
      AtAAH
      Gene namesi
      Name:AAH
      Ordered Locus Names:At4g20070
      ORF Names:F18F4.170
      OrganismiArabidopsis thaliana (Mouse-ear cress)
      Taxonomic identifieri3702 [NCBI]
      Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
      Proteomesi
      • UP000006548 Componenti: Chromosome 4

      Organism-specific databases

      TAIRiAT4G20070.

      Subcellular locationi

      • Endoplasmic reticulum 1 Publication

      GO - Cellular componenti

      • endoplasmic reticulum Source: TAIR
      Complete GO annotation...

      Keywords - Cellular componenti

      Endoplasmic reticulum

      Pathology & Biotechi

      Disruption phenotypei

      No visible phenotype under normal growth conditions, but mutant plants are unable to grow on a medium containing allantoin as the sole nitrogen source and accumulate allantoate.3 Publications

      PTM / Processingi

      Molecule processing

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Signal peptidei1 – 53Sequence analysisAdd BLAST53
      ChainiPRO_000031583854 – 525Allantoate deiminaseAdd BLAST472

      Proteomic databases

      PaxDbiO49434.

      Expressioni

      Tissue specificityi

      Expressed in seedlings, roots, stems, leaves, flowers, siliques and seeds.1 Publication

      Gene expression databases

      GenevisibleiO49434. AT.

      Interactioni

      Subunit structurei

      Homodimer.1 Publication

      Protein-protein interaction databases

      STRINGi3702.AT4G20070.1.

      Structurei

      3D structure databases

      ProteinModelPortaliO49434.
      SMRiO49434.
      ModBaseiSearch...
      MobiDBiSearch...

      Family & Domainsi

      Compositional bias

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Compositional biasi7 – 74Ser-richAdd BLAST68
      Compositional biasi14 – 33His-richAdd BLAST20

      Sequence similaritiesi

      Belongs to the peptidase M20A family.Curated

      Keywords - Domaini

      Signal

      Phylogenomic databases

      eggNOGiENOG410II0M. Eukaryota.
      COG0624. LUCA.
      HOGENOMiHOG000241291.
      InParanoidiO49434.
      KOiK02083.
      OMAiIVNFGDE.
      OrthoDBiEOG093608AX.
      PhylomeDBiO49434.

      Family and domain databases

      CDDicd03884. M20_bAS. 1 hit.
      Gene3Di3.30.70.360. 1 hit.
      InterProiIPR010158. Amidase_Cbmase.
      IPR001261. ArgE/DapE_CS.
      IPR002933. Peptidase_M20.
      IPR011650. Peptidase_M20_dimer.
      [Graphical view]
      PfamiPF07687. M20_dimer. 1 hit.
      PF01546. Peptidase_M20. 1 hit.
      [Graphical view]
      SUPFAMiSSF55031. SSF55031. 1 hit.
      TIGRFAMsiTIGR01879. hydantase. 1 hit.
      PROSITEiPS00758. ARGE_DAPE_CPG2_1. 1 hit.
      [Graphical view]

      Sequencei

      Sequence statusi: Complete.

      Sequence processingi: The displayed sequence is further processed into a mature form.

      O49434-1 [UniParc]FASTAAdd to basket

      « Hide

              10         20         30         40         50
      MAVPHPSSSS SRSHPFLSHV YHTSFHHHHH HNHPSLVLFW CLVFSLLSPL
      60 70 80 90 100
      ALSSSSSSSS SSSDSSSSSS SHISLGIGET EGTKHDLHQA ILRDEAVARL
      110 120 130 140 150
      HELGQVSDAA THLERTFMSP ASIRAIPLIR GWMEDAGLST WVDYMGNVHG
      160 170 180 190 200
      RVEPKNGSSQ ALLIGSHMDT VIDAGKYDGS LGIISAISAL KVLKIDGRLG
      210 220 230 240 250
      ELKRPVEVIA FSDEEGVRFQ STFLGSAALA GIMPVSRLEV TDKSGISVQD
      260 270 280 290 300
      ALKENSIDIT DENLMQLKYD PASVWGYVEV HIEQGPVLEW VGYPLGVVKG
      310 320 330 340 350
      IAGQTRLKVT VKGSQGHAGT VPMSMRQDPM TGAAELIVLL ESVCKNPKDY
      360 370 380 390 400
      LSCNVQCNED TVESLANSLV CTVGEISTWP SASNVIPGQV TFTVDLRTID
      410 420 430 440 450
      DVGRKAILHD LSTRMYQICD KRSLLCSIER KHDADAVMSD PQLSLQLKSA
      460 470 480 490 500
      AQSALKKMTG EVQDEVPVLM SGAGHDAMAM AHLTKVGMLF VRCRGGISHS
      510 520
      PAEHVLDDDV GAAGLAILEF LESQM
      Length:525
      Mass (Da):56,524
      Last modified:May 1, 2000 - v2
      Checksum:i8744B53F4C28ADE0
      GO

      Sequence databases

      Select the link destinations:
      EMBLi
      GenBanki
      DDBJi
      Links Updated
      AL021637 Genomic DNA. Translation: CAA16615.2.
      AL161552 Genomic DNA. Translation: CAB79007.1.
      CP002687 Genomic DNA. Translation: AEE84269.1.
      BT025334 mRNA. Translation: ABF57290.1.
      PIRiF85227.
      RefSeqiNP_193740.1. NM_118126.4.
      UniGeneiAt.32752.

      Genome annotation databases

      EnsemblPlantsiAT4G20070.1; AT4G20070.1; AT4G20070.
      GeneIDi827752.
      GrameneiAT4G20070.1; AT4G20070.1; AT4G20070.
      KEGGiath:AT4G20070.

      Cross-referencesi

      Sequence databases

      Select the link destinations:
      EMBLi
      GenBanki
      DDBJi
      Links Updated
      AL021637 Genomic DNA. Translation: CAA16615.2.
      AL161552 Genomic DNA. Translation: CAB79007.1.
      CP002687 Genomic DNA. Translation: AEE84269.1.
      BT025334 mRNA. Translation: ABF57290.1.
      PIRiF85227.
      RefSeqiNP_193740.1. NM_118126.4.
      UniGeneiAt.32752.

      3D structure databases

      ProteinModelPortaliO49434.
      SMRiO49434.
      ModBaseiSearch...
      MobiDBiSearch...

      Protein-protein interaction databases

      STRINGi3702.AT4G20070.1.

      Protein family/group databases

      MEROPSiM20.A07.

      Proteomic databases

      PaxDbiO49434.

      Protocols and materials databases

      Structural Biology KnowledgebaseSearch...

      Genome annotation databases

      EnsemblPlantsiAT4G20070.1; AT4G20070.1; AT4G20070.
      GeneIDi827752.
      GrameneiAT4G20070.1; AT4G20070.1; AT4G20070.
      KEGGiath:AT4G20070.

      Organism-specific databases

      TAIRiAT4G20070.

      Phylogenomic databases

      eggNOGiENOG410II0M. Eukaryota.
      COG0624. LUCA.
      HOGENOMiHOG000241291.
      InParanoidiO49434.
      KOiK02083.
      OMAiIVNFGDE.
      OrthoDBiEOG093608AX.
      PhylomeDBiO49434.

      Enzyme and pathway databases

      BioCyciARA:AT4G20070-MONOMER.
      MetaCyc:AT4G20070-MONOMER.
      BRENDAi3.5.3.9. 399.

      Miscellaneous databases

      PROiO49434.

      Gene expression databases

      GenevisibleiO49434. AT.

      Family and domain databases

      CDDicd03884. M20_bAS. 1 hit.
      Gene3Di3.30.70.360. 1 hit.
      InterProiIPR010158. Amidase_Cbmase.
      IPR001261. ArgE/DapE_CS.
      IPR002933. Peptidase_M20.
      IPR011650. Peptidase_M20_dimer.
      [Graphical view]
      PfamiPF07687. M20_dimer. 1 hit.
      PF01546. Peptidase_M20. 1 hit.
      [Graphical view]
      SUPFAMiSSF55031. SSF55031. 1 hit.
      TIGRFAMsiTIGR01879. hydantase. 1 hit.
      PROSITEiPS00758. ARGE_DAPE_CPG2_1. 1 hit.
      [Graphical view]
      ProtoNetiSearch...

      Entry informationi

      Entry nameiAAH_ARATH
      AccessioniPrimary (citable) accession number: O49434
      Entry historyi
      Integrated into UniProtKB/Swiss-Prot: January 15, 2008
      Last sequence update: May 1, 2000
      Last modified: November 30, 2016
      This is version 112 of the entry and version 2 of the sequence. [Complete history]
      Entry statusiReviewed (UniProtKB/Swiss-Prot)
      Annotation programPlant Protein Annotation Program

      Miscellaneousi

      Keywords - Technical termi

      Complete proteome, Reference proteome

      Documents

      1. Arabidopsis thaliana
        Arabidopsis thaliana: entries and gene names
      2. Peptidase families
        Classification of peptidase families and list of entries
      3. SIMILARITY comments
        Index of protein domains and families

      Similar proteinsi

      Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
      100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
      90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
      50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.